Eosinophil granule proteins ECP and EPX as markers for a potential early-stage inflammatory lesion in female genital schistosomiasis (FGS)

Charles Emile Ramarokoto; Anna M. O. Kildemoes; Bodo Sahondra Randrianasolo; Pascaline Ravoniarimbinina; Vololomboahangy Elisabeth Ravaoalimalala; Peter Leutscher; Eyrun Floerecke Kjetland; Birgitte J Vennervald

doi:10.1371/journal.pntd.0002974

Eosinophil granule proteins ECP and EPX as markers for a potential early-stage inflammatory lesion in female genital schistosomiasis (FGS)

Charles Emile Ramarokoto, Anna M. O. Kildemoes, Bodo Sahondra Randrianasolo, Pascaline Ravoniarimbinina, Vololomboahangy Elisabeth Ravaoalimalala, Peter Leutscher, Eyrun Floerecke Kjetland, Birgitte J Vennervald

7 Citations (Scopus)

695 Downloads (Pure)

Abstract

BACKGROUND: Genital granulomas induced by Schistosoma haematobium eggs can manifest as different lesion types visible by colposcopy; rubbery papules (RP), homogenous sandy patches (HSP) and grainy sandy patches (GSP). Pronounced tissue eosinophilia is a candidate marker for active S. haematobium pathology, as viable schistosome egg granulomas often are eosinophil rich. Here it was investigated whether eosinophil granule proteins ECP (eosinophil cationic protein) and EPX (eosinophil protein-X) in urine and genital lavage can be used as markers for active FGS lesions.

METHODS: Uro-genital samples from 118 Malagasy women were analysed for ECP and EPX by standard sandwich avidin/biotin amplified ELISA.

PRINCIPAL FINDINGS: The women with RP lesions had significantly higher levels of ECP and EPX in both lavage and urine. Furthermore, women with RP lesions were significantly younger than those with GSP. This could indicate that RP lesions might be more recently established and thus represent an earlier inflammatory lesion stage.

CONCLUSION: ECP in genital lavage might be a future tool aiding the identification of FGS pathology at a stage where reversibility remains a possibility following praziquantel treatment.

Original language	English
Article number	e2974
Journal	P L o S Neglected Tropical Diseases (Online)
Volume	8
Issue number	7
Number of pages	7
ISSN	1935-2735
DOIs	https://doi.org/10.1371/journal.pntd.0002974
Publication status	Published - Jul 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1371/journal.pntd.0002974

Ramarakoto2014Final published version, 436 KB

Cite this

Ramarokoto, C. E., Kildemoes, A. M. O., Randrianasolo, B. S., Ravoniarimbinina, P., Ravaoalimalala, V. E., Leutscher, P., Kjetland, E. F., & Vennervald, B. J. (2014). Eosinophil granule proteins ECP and EPX as markers for a potential early-stage inflammatory lesion in female genital schistosomiasis (FGS). P L o S Neglected Tropical Diseases (Online), 8(7), Article e2974. https://doi.org/10.1371/journal.pntd.0002974

Eosinophil granule proteins ECP and EPX as markers for a potential early-stage inflammatory lesion in female genital schistosomiasis (FGS). / Ramarokoto, Charles Emile; Kildemoes, Anna M. O.; Randrianasolo, Bodo Sahondra et al.
In: P L o S Neglected Tropical Diseases (Online), Vol. 8, No. 7, e2974, 07.2014.

Research output: Contribution to journal › Journal article › Research › peer-review

Ramarokoto, CE, Kildemoes, AMO, Randrianasolo, BS, Ravoniarimbinina, P, Ravaoalimalala, VE, Leutscher, P, Kjetland, EF & Vennervald, BJ 2014, 'Eosinophil granule proteins ECP and EPX as markers for a potential early-stage inflammatory lesion in female genital schistosomiasis (FGS)', P L o S Neglected Tropical Diseases (Online), vol. 8, no. 7, e2974. https://doi.org/10.1371/journal.pntd.0002974

@article{e75a90e9ecd94b3f8cfb64eda08a4586,

title = "Eosinophil granule proteins ECP and EPX as markers for a potential early-stage inflammatory lesion in female genital schistosomiasis (FGS)",

abstract = "BACKGROUND: Genital granulomas induced by Schistosoma haematobium eggs can manifest as different lesion types visible by colposcopy; rubbery papules (RP), homogenous sandy patches (HSP) and grainy sandy patches (GSP). Pronounced tissue eosinophilia is a candidate marker for active S. haematobium pathology, as viable schistosome egg granulomas often are eosinophil rich. Here it was investigated whether eosinophil granule proteins ECP (eosinophil cationic protein) and EPX (eosinophil protein-X) in urine and genital lavage can be used as markers for active FGS lesions.METHODS: Uro-genital samples from 118 Malagasy women were analysed for ECP and EPX by standard sandwich avidin/biotin amplified ELISA.PRINCIPAL FINDINGS: The women with RP lesions had significantly higher levels of ECP and EPX in both lavage and urine. Furthermore, women with RP lesions were significantly younger than those with GSP. This could indicate that RP lesions might be more recently established and thus represent an earlier inflammatory lesion stage.CONCLUSION: ECP in genital lavage might be a future tool aiding the identification of FGS pathology at a stage where reversibility remains a possibility following praziquantel treatment.",

author = "Ramarokoto, {Charles Emile} and Kildemoes, {Anna M. O.} and Randrianasolo, {Bodo Sahondra} and Pascaline Ravoniarimbinina and Ravaoalimalala, {Vololomboahangy Elisabeth} and Peter Leutscher and Kjetland, {Eyrun Floerecke} and Vennervald, {Birgitte J}",

year = "2014",

month = jul,

doi = "10.1371/journal.pntd.0002974",

language = "English",

volume = "8",

journal = "P L o S Neglected Tropical Diseases (Online)",

issn = "1935-2735",

publisher = "Public Library of Science",

number = "7",

}

TY - JOUR

T1 - Eosinophil granule proteins ECP and EPX as markers for a potential early-stage inflammatory lesion in female genital schistosomiasis (FGS)

AU - Ramarokoto, Charles Emile

AU - Kildemoes, Anna M. O.

AU - Randrianasolo, Bodo Sahondra

AU - Ravoniarimbinina, Pascaline

AU - Ravaoalimalala, Vololomboahangy Elisabeth

AU - Leutscher, Peter

AU - Kjetland, Eyrun Floerecke

AU - Vennervald, Birgitte J

PY - 2014/7

Y1 - 2014/7

N2 - BACKGROUND: Genital granulomas induced by Schistosoma haematobium eggs can manifest as different lesion types visible by colposcopy; rubbery papules (RP), homogenous sandy patches (HSP) and grainy sandy patches (GSP). Pronounced tissue eosinophilia is a candidate marker for active S. haematobium pathology, as viable schistosome egg granulomas often are eosinophil rich. Here it was investigated whether eosinophil granule proteins ECP (eosinophil cationic protein) and EPX (eosinophil protein-X) in urine and genital lavage can be used as markers for active FGS lesions.METHODS: Uro-genital samples from 118 Malagasy women were analysed for ECP and EPX by standard sandwich avidin/biotin amplified ELISA.PRINCIPAL FINDINGS: The women with RP lesions had significantly higher levels of ECP and EPX in both lavage and urine. Furthermore, women with RP lesions were significantly younger than those with GSP. This could indicate that RP lesions might be more recently established and thus represent an earlier inflammatory lesion stage.CONCLUSION: ECP in genital lavage might be a future tool aiding the identification of FGS pathology at a stage where reversibility remains a possibility following praziquantel treatment.

AB - BACKGROUND: Genital granulomas induced by Schistosoma haematobium eggs can manifest as different lesion types visible by colposcopy; rubbery papules (RP), homogenous sandy patches (HSP) and grainy sandy patches (GSP). Pronounced tissue eosinophilia is a candidate marker for active S. haematobium pathology, as viable schistosome egg granulomas often are eosinophil rich. Here it was investigated whether eosinophil granule proteins ECP (eosinophil cationic protein) and EPX (eosinophil protein-X) in urine and genital lavage can be used as markers for active FGS lesions.METHODS: Uro-genital samples from 118 Malagasy women were analysed for ECP and EPX by standard sandwich avidin/biotin amplified ELISA.PRINCIPAL FINDINGS: The women with RP lesions had significantly higher levels of ECP and EPX in both lavage and urine. Furthermore, women with RP lesions were significantly younger than those with GSP. This could indicate that RP lesions might be more recently established and thus represent an earlier inflammatory lesion stage.CONCLUSION: ECP in genital lavage might be a future tool aiding the identification of FGS pathology at a stage where reversibility remains a possibility following praziquantel treatment.

U2 - 10.1371/journal.pntd.0002974

DO - 10.1371/journal.pntd.0002974

M3 - Journal article

C2 - 25033206

SN - 1935-2735

VL - 8

JO - P L o S Neglected Tropical Diseases (Online)

JF - P L o S Neglected Tropical Diseases (Online)

IS - 7

M1 - e2974

ER -

Eosinophil granule proteins ECP and EPX as markers for a potential early-stage inflammatory lesion in female genital schistosomiasis (FGS)

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this