Abstract
Glycosyltransferases are important biological catalysts in cellular systems generating complex cell surface glycans involved in adhesion and signaling processes. Recent advances in glycoscience have increased the demands to access significant amount of glycans representing the glycome. Glycosyltransferases are now playing a key role for in vitro synthesis of oligosaccharides and the bacterial genome are increasingly utilized for cloning and over expression of active transferases in glycosylation reactions. This chapter highlights the recent progress towards preparative synthesis of oligosaccharides representing terminal sequences of glycoproteins and glycolipids using recombinant transferases. Transferases are also being explored in the context of solid-phase synthesis, immobilized on resins and over expression in vivo by engineered bacteria.
| Original language | English |
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| Title of host publication | Glycoscience : Chemistry and Chemical Biology |
| Number of pages | 25 |
| Volume | 5 |
| Place of Publication | Springer Berlin Heidelberg |
| Publisher | Springer |
| Publication date | 2008 |
| Edition | 2nd ed |
| Pages | 1361-1385 |
| ISBN (Print) | 978-3-540-36154-1 |
| ISBN (Electronic) | 978-3-540-30429-6 |
| Publication status | Published - 2008 |