TY - JOUR
T1 - Enrichment and characterization of an environmental microbial consortium displaying efficient keratinolytic activity
AU - Kang, Dingrong
AU - Herschend, Jakob
AU - Abu Al-Soud, Waleed
AU - Mortensen, Martin Steen
AU - Gonzalo, Milena
AU - Jacquiod, Samuel Jehan Auguste
AU - Sørensen, Søren Johannes
PY - 2018/12
Y1 - 2018/12
N2 - Keratin refers to a group of insoluble and recalcitrant protein materials. Slaughterhouses produce large amount of keratinous byproducts, which are either disposed or poorly valorized through costly thermochemical processes for animal feed formulation. Learning from nature, keratinolytic microbial consortia stand as a cost-efficient and environmental friendly way to valorize this recalcitrant resource. Directed selection was applied to enrich soil-born microbial consortia, using sequential batch cultivations in keratin medium, while measuring enzymes activity and monitoring consortia compositions via 16S rRNA gene amplicon sequencing. A promising microbial consortium KMCG6, featuring mainly members of Bacteroidetes and Proteobacteria, was obtained. It possessed keratinolytic activity with <25% residual substrate remaining, which also displayed a high degradation reproducibility level after long-term cryopreservation. This work represents an advance in the field of α-keratin degradation with potential for practical applications.
AB - Keratin refers to a group of insoluble and recalcitrant protein materials. Slaughterhouses produce large amount of keratinous byproducts, which are either disposed or poorly valorized through costly thermochemical processes for animal feed formulation. Learning from nature, keratinolytic microbial consortia stand as a cost-efficient and environmental friendly way to valorize this recalcitrant resource. Directed selection was applied to enrich soil-born microbial consortia, using sequential batch cultivations in keratin medium, while measuring enzymes activity and monitoring consortia compositions via 16S rRNA gene amplicon sequencing. A promising microbial consortium KMCG6, featuring mainly members of Bacteroidetes and Proteobacteria, was obtained. It possessed keratinolytic activity with <25% residual substrate remaining, which also displayed a high degradation reproducibility level after long-term cryopreservation. This work represents an advance in the field of α-keratin degradation with potential for practical applications.
U2 - 10.1016/j.biortech.2018.09.006
DO - 10.1016/j.biortech.2018.09.006
M3 - Journal article
C2 - 30236907
SN - 0960-8524
VL - 270
SP - 303
EP - 310
JO - Bioresource Technology
JF - Bioresource Technology
ER -