Engineering of plastids to optimize the production of high-value metabolites and proteins

Poul Erik Jensen; Lars B Scharff

doi:10.1016/j.copbio.2019.01.009

Engineering of plastids to optimize the production of high-value metabolites and proteins

Poul Erik Jensen, Lars B Scharff

Section for Molecular Plant Biology

8 Citations (Scopus)

Abstract

Plastids are interesting targets for metabolic engineering using the tools of synthetic biology. Plastids carry their own genome, which can be manipulated genetically in many algae and plants. Incorporating foreign genes into the plastid genome offers valuable benefits, such as high-level foreign protein expression and the absence of gene silencing. Here, we review progress in bioengineering of chloroplasts to produce valuable metabolites and proteins. Various strategies for enhancing yields of desired products, including design of operons, fusion proteins for improved translational efficiency, protein scaffolding, metabolic channeling and storage, are described. Efforts to control plastid differentiation also offer promising ways of turning plastids into controllable bio-factories, and the construction of synthetic plastids optimized for specific functions would be a major advance.

Original language	English
Journal	Current Opinion in Biotechnology
Volume	59
Pages (from-to)	8-15
Number of pages	8
ISSN	0958-1669
DOIs	https://doi.org/10.1016/j.copbio.2019.01.009
Publication status	Published - Oct 2019

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title = "Engineering of plastids to optimize the production of high-value metabolites and proteins",

abstract = "Plastids are interesting targets for metabolic engineering using the tools of synthetic biology. Plastids carry their own genome, which can be manipulated genetically in many algae and plants. Incorporating foreign genes into the plastid genome offers valuable benefits, such as high-level foreign protein expression and the absence of gene silencing. Here, we review progress in bioengineering of chloroplasts to produce valuable metabolites and proteins. Various strategies for enhancing yields of desired products, including design of operons, fusion proteins for improved translational efficiency, protein scaffolding, metabolic channeling and storage, are described. Efforts to control plastid differentiation also offer promising ways of turning plastids into controllable bio-factories, and the construction of synthetic plastids optimized for specific functions would be a major advance.",

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AU - Jensen, Poul Erik

AU - Scharff, Lars B

PY - 2019/10

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N2 - Plastids are interesting targets for metabolic engineering using the tools of synthetic biology. Plastids carry their own genome, which can be manipulated genetically in many algae and plants. Incorporating foreign genes into the plastid genome offers valuable benefits, such as high-level foreign protein expression and the absence of gene silencing. Here, we review progress in bioengineering of chloroplasts to produce valuable metabolites and proteins. Various strategies for enhancing yields of desired products, including design of operons, fusion proteins for improved translational efficiency, protein scaffolding, metabolic channeling and storage, are described. Efforts to control plastid differentiation also offer promising ways of turning plastids into controllable bio-factories, and the construction of synthetic plastids optimized for specific functions would be a major advance.

AB - Plastids are interesting targets for metabolic engineering using the tools of synthetic biology. Plastids carry their own genome, which can be manipulated genetically in many algae and plants. Incorporating foreign genes into the plastid genome offers valuable benefits, such as high-level foreign protein expression and the absence of gene silencing. Here, we review progress in bioengineering of chloroplasts to produce valuable metabolites and proteins. Various strategies for enhancing yields of desired products, including design of operons, fusion proteins for improved translational efficiency, protein scaffolding, metabolic channeling and storage, are described. Efforts to control plastid differentiation also offer promising ways of turning plastids into controllable bio-factories, and the construction of synthetic plastids optimized for specific functions would be a major advance.

U2 - 10.1016/j.copbio.2019.01.009

DO - 10.1016/j.copbio.2019.01.009

M3 - Review

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SP - 8

EP - 15

JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

ER -

Engineering of plastids to optimize the production of high-value metabolites and proteins

Abstract

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