Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots

Lars R. Ingerslev; Ida Donkin; Odile Fabre; Soetkin Versteyhe; Mie Mechta; Pattarawan Pattamaprapanont; Brynjulf Mortensen; Nikolaj Thure Krarup; Romain Barrès

doi:10.1186/s13148-018-0446-7

Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots

Lars R. Ingerslev, Ida Donkin, Odile Fabre, Soetkin Versteyhe, Mie Mechta, Pattarawan Pattamaprapanont, Brynjulf Mortensen, Nikolaj Thure Krarup, Romain Barrès

33 Citations (Scopus)

101 Downloads (Pure)

Abstract

Remodeling of the sperm epigenome by lifestyle factors before conception could account for altered metabolism in the next generation offspring. Here, we hypothesized that endurance training changes the epigenome of human spermatozoa. Using small RNA (sRNA) sequencing and reduced representation bisulfite sequencing (RRBS), we, respectively, investigated sRNA expression and DNA methylation in pure fractions of motile spermatozoa collected from young healthy individuals before, after 6 weeks of endurance training and after 3 months without exercise. Expression of 8 PIWI interacting RNA were changed by exercise training. RRBS analysis revealed 330 differentially methylated regions (DMRs) after training and 303 DMRs after the detraining period, which were, in both conditions, enriched at close vicinity of transcription start sites. Ontology analysis of genes located at proximity of DMRs returned terms related to neurological function at the trained state and, to a much lesser extent, at the detrained state. Our study reveal that short-term endurance training induces marked remodeling of the sperm epigenome, and identify genes related to the development of the central nervous system as potential hot spots for epigenetic variation upon environmental stress.

Original language	English
Article number	12
Journal	Clin Epigenetics
Volume	10
Issue number	1
Pages (from-to)	1-11
ISSN	1868-7075
DOIs	https://doi.org/10.1186/s13148-018-0446-7
Publication status	Published - 2018

Keywords

Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1186/s13148-018-0446-7

Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspotsFinal published version, 2.41 MB

Cite this

@article{c17b25c1b70c47f89afff2a97b0acb77,

title = "Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots",

abstract = "Remodeling of the sperm epigenome by lifestyle factors before conception could account for altered metabolism in the next generation offspring. Here, we hypothesized that endurance training changes the epigenome of human spermatozoa. Using small RNA (sRNA) sequencing and reduced representation bisulfite sequencing (RRBS), we, respectively, investigated sRNA expression and DNA methylation in pure fractions of motile spermatozoa collected from young healthy individuals before, after 6 weeks of endurance training and after 3 months without exercise. Expression of 8 PIWI interacting RNA were changed by exercise training. RRBS analysis revealed 330 differentially methylated regions (DMRs) after training and 303 DMRs after the detraining period, which were, in both conditions, enriched at close vicinity of transcription start sites. Ontology analysis of genes located at proximity of DMRs returned terms related to neurological function at the trained state and, to a much lesser extent, at the detrained state. Our study reveal that short-term endurance training induces marked remodeling of the sperm epigenome, and identify genes related to the development of the central nervous system as potential hot spots for epigenetic variation upon environmental stress.",

keywords = "Journal Article",

author = "Ingerslev, {Lars R.} and Ida Donkin and Odile Fabre and Soetkin Versteyhe and Mie Mechta and Pattarawan Pattamaprapanont and Brynjulf Mortensen and Krarup, {Nikolaj Thure} and Romain Barr{\`e}s",

year = "2018",

doi = "10.1186/s13148-018-0446-7",

language = "English",

volume = "10",

pages = "1--11",

journal = "Clin Epigenetics",

issn = "1868-7075",

publisher = "BioMed Central Ltd.",

number = "1",

}

TY - JOUR

T1 - Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots

AU - Ingerslev, Lars R.

AU - Donkin, Ida

AU - Fabre, Odile

AU - Versteyhe, Soetkin

AU - Mechta, Mie

AU - Pattamaprapanont, Pattarawan

AU - Mortensen, Brynjulf

AU - Krarup, Nikolaj Thure

AU - Barrès, Romain

PY - 2018

Y1 - 2018

N2 - Remodeling of the sperm epigenome by lifestyle factors before conception could account for altered metabolism in the next generation offspring. Here, we hypothesized that endurance training changes the epigenome of human spermatozoa. Using small RNA (sRNA) sequencing and reduced representation bisulfite sequencing (RRBS), we, respectively, investigated sRNA expression and DNA methylation in pure fractions of motile spermatozoa collected from young healthy individuals before, after 6 weeks of endurance training and after 3 months without exercise. Expression of 8 PIWI interacting RNA were changed by exercise training. RRBS analysis revealed 330 differentially methylated regions (DMRs) after training and 303 DMRs after the detraining period, which were, in both conditions, enriched at close vicinity of transcription start sites. Ontology analysis of genes located at proximity of DMRs returned terms related to neurological function at the trained state and, to a much lesser extent, at the detrained state. Our study reveal that short-term endurance training induces marked remodeling of the sperm epigenome, and identify genes related to the development of the central nervous system as potential hot spots for epigenetic variation upon environmental stress.

AB - Remodeling of the sperm epigenome by lifestyle factors before conception could account for altered metabolism in the next generation offspring. Here, we hypothesized that endurance training changes the epigenome of human spermatozoa. Using small RNA (sRNA) sequencing and reduced representation bisulfite sequencing (RRBS), we, respectively, investigated sRNA expression and DNA methylation in pure fractions of motile spermatozoa collected from young healthy individuals before, after 6 weeks of endurance training and after 3 months without exercise. Expression of 8 PIWI interacting RNA were changed by exercise training. RRBS analysis revealed 330 differentially methylated regions (DMRs) after training and 303 DMRs after the detraining period, which were, in both conditions, enriched at close vicinity of transcription start sites. Ontology analysis of genes located at proximity of DMRs returned terms related to neurological function at the trained state and, to a much lesser extent, at the detrained state. Our study reveal that short-term endurance training induces marked remodeling of the sperm epigenome, and identify genes related to the development of the central nervous system as potential hot spots for epigenetic variation upon environmental stress.

KW - Journal Article

U2 - 10.1186/s13148-018-0446-7

DO - 10.1186/s13148-018-0446-7

M3 - Journal article

C2 - 29416570

SN - 1868-7075

VL - 10

SP - 1

EP - 11

JO - Clin Epigenetics

JF - Clin Epigenetics

IS - 1

M1 - 12

ER -

Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this