TY - JOUR
T1 - Toxocara cati larvae persist and retain high infectivity in muscles of experimentally infected chickens
AU - Taira, Kensuke
AU - Saitoh, Yasuhide
AU - Kapel, Christian Moliin Outzen
PY - 2011/8/25
Y1 - 2011/8/25
N2 - The distribution of Toxocara cati larvae in the organs of chickens (n= 31), experimentally inoculated with 3000 embryonated eggs, was examined 1, 2, 3, 7, 29, 86 and 175-176. days post-infection (dpi), and the infectivity of recovered larvae was evaluated by bioassay in mice. The duodenum, liver, lungs, heart, brain, pectoral muscles (white meat), and hindlimb muscles (red meat) of the chickens were HCl-pepsin digested for larval recovery. Larvae were recovered from all chickens [mean = 220.4 ± 114.9 (SD)], and although no decrease of total larval recovery was observed over time, predilection sites changed: liver (92.6% of larval recovery) at 1 dpi; lungs (77.3%) and liver (20.9%) at 2 dpi; lungs (80.8%), muscles (9.4%), and liver (8.5%) at 3 dpi; muscles (52.0%) and lungs (45.6%) at 7 dpi, whereafter most larvae were recovered from muscles: 29 dpi (99.5%), 86 dpi (99.3%) and 175-176 dpi (99.6%). In the bioassay, 52.9% of larvae from 175 to 176. days old infections in chickens established in mice, which demonstrates that T. cati larvae retain infective in the muscles of chickens for half a year. These results highlight the zoonotic potential of poultry meat as a causative agent of human toxocarosis.
AB - The distribution of Toxocara cati larvae in the organs of chickens (n= 31), experimentally inoculated with 3000 embryonated eggs, was examined 1, 2, 3, 7, 29, 86 and 175-176. days post-infection (dpi), and the infectivity of recovered larvae was evaluated by bioassay in mice. The duodenum, liver, lungs, heart, brain, pectoral muscles (white meat), and hindlimb muscles (red meat) of the chickens were HCl-pepsin digested for larval recovery. Larvae were recovered from all chickens [mean = 220.4 ± 114.9 (SD)], and although no decrease of total larval recovery was observed over time, predilection sites changed: liver (92.6% of larval recovery) at 1 dpi; lungs (77.3%) and liver (20.9%) at 2 dpi; lungs (80.8%), muscles (9.4%), and liver (8.5%) at 3 dpi; muscles (52.0%) and lungs (45.6%) at 7 dpi, whereafter most larvae were recovered from muscles: 29 dpi (99.5%), 86 dpi (99.3%) and 175-176 dpi (99.6%). In the bioassay, 52.9% of larvae from 175 to 176. days old infections in chickens established in mice, which demonstrates that T. cati larvae retain infective in the muscles of chickens for half a year. These results highlight the zoonotic potential of poultry meat as a causative agent of human toxocarosis.
U2 - 10.1016/j.vetpar.2011.03.020
DO - 10.1016/j.vetpar.2011.03.020
M3 - Journal article
C2 - 21482027
SN - 0304-4017
VL - 180
SP - 287
EP - 291
JO - Veterinary Parasitology
JF - Veterinary Parasitology
IS - 3-4
ER -