In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Evandro F. Fang; Konstantinos Palikaras; Nuo Sun; Elayne M. Fivenson; Ryan D. Spangler; Jesse S. Kerr; Stephanie A. Cordonnier; Yujun Hou; Eszter Dombi; Henok Kassahun; Nektarios Tavernarakis; Joanna Poulton; Hilde Nilsen; Vilhelm A. Bohr

doi:10.3791/56301

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Evandro F. Fang, Konstantinos Palikaras, Nuo Sun, Elayne M. Fivenson, Ryan D. Spangler, Jesse S. Kerr, Stephanie A. Cordonnier, Yujun Hou, Eszter Dombi, Henok Kassahun, Nektarios Tavernarakis, Joanna Poulton, Hilde Nilsen, Vilhelm A. Bohr

15 Citations (Scopus)

Abstract

Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.

Original language	English
Article number	e56301
Journal	Journal of Visualized Experiments
Issue number	129
ISSN	1940-087X
DOIs	https://doi.org/10.3791/56301
Publication status	Published - 22 Nov 2017

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Fang, E. F., Palikaras, K., Sun, N., Fivenson, E. M., Spangler, R. D., Kerr, J. S., Cordonnier, S. A., Hou, Y., Dombi, E., Kassahun, H., Tavernarakis, N., Poulton, J., Nilsen, H., & Bohr, V. A. (2017). In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice. Journal of Visualized Experiments, (129), Article e56301. https://doi.org/10.3791/56301

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title = "In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice",

abstract = "Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.",

author = "Fang, {Evandro F.} and Konstantinos Palikaras and Nuo Sun and Fivenson, {Elayne M.} and Spangler, {Ryan D.} and Kerr, {Jesse S.} and Cordonnier, {Stephanie A.} and Yujun Hou and Eszter Dombi and Henok Kassahun and Nektarios Tavernarakis and Joanna Poulton and Hilde Nilsen and Bohr, {Vilhelm A.}",

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AU - Fang, Evandro F.

AU - Palikaras, Konstantinos

AU - Sun, Nuo

AU - Fivenson, Elayne M.

AU - Spangler, Ryan D.

AU - Kerr, Jesse S.

AU - Cordonnier, Stephanie A.

AU - Hou, Yujun

AU - Dombi, Eszter

AU - Kassahun, Henok

AU - Tavernarakis, Nektarios

AU - Poulton, Joanna

AU - Nilsen, Hilde

AU - Bohr, Vilhelm A.

PY - 2017/11/22

Y1 - 2017/11/22

N2 - Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.

AB - Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.

U2 - 10.3791/56301

DO - 10.3791/56301

M3 - Journal article

C2 - 29286376

SN - 1940-087X

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

IS - 129

M1 - e56301

ER -

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Abstract

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