EMG-normalised kinase activation during exercise is higher in human gastrocnemius compared to soleus muscle

Thomas Elbenhardt Jensen; Robin Leutert; Søren T Rasmussen; Joshua Roger Mouatt; Mette Landau Brabæk Christiansen; Bente Rona Jensen; Erik Richter

doi:10.1371/journal.pone.0031054

EMG-normalised kinase activation during exercise is higher in human gastrocnemius compared to soleus muscle

Thomas Elbenhardt Jensen, Robin Leutert, Søren T Rasmussen, Joshua Roger Mouatt, Mette Landau Brabæk Christiansen, Bente Rona Jensen, Erik Richter

18 Citations (Scopus)

Abstract

In mice, certain proteins show a highly confined expression in specific muscle groups. Also, resting and exercise/contraction-induced phosphorylation responses are higher in rat skeletal muscle with low mitochondrial content compared to muscles with high mitochondrial content, possibly related to differential reactive oxygen species (ROS)-scavenging ability or resting glycogen content. To evaluate these parameters in humans, biopsies from soleus, gastrocnemius and vastus lateralis muscles were taken before and after a 45 min inclined (15%) walking exercise bout at 69% VO2(max) aimed at simultaneously activating soleus and gastrocnemius in a comparable dynamic work-pattern. Hexokinase II and GLUT4 were 46-59% and 26-38% higher (p

Original language	English
Journal	P L o S One
Volume	7
Issue number	2
Pages (from-to)	e31054
Number of pages	8
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0031054
Publication status	Published - 8 Feb 2012

Keywords

Electromyography
Enzyme Activation
Exercise
Glucose Transporter Type 4
Hexokinase
Humans
Muscle, Skeletal
Phosphorylation
Protein Kinases
Signal Transduction

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title = "EMG-normalised kinase activation during exercise is higher in human gastrocnemius compared to soleus muscle",

abstract = "In mice, certain proteins show a highly confined expression in specific muscle groups. Also, resting and exercise/contraction-induced phosphorylation responses are higher in rat skeletal muscle with low mitochondrial content compared to muscles with high mitochondrial content, possibly related to differential reactive oxygen species (ROS)-scavenging ability or resting glycogen content. To evaluate these parameters in humans, biopsies from soleus, gastrocnemius and vastus lateralis muscles were taken before and after a 45 min inclined (15%) walking exercise bout at 69% VO2(max) aimed at simultaneously activating soleus and gastrocnemius in a comparable dynamic work-pattern. Hexokinase II and GLUT4 were 46-59% and 26-38% higher (p",

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AU - Jensen, Thomas Elbenhardt

AU - Leutert, Robin

AU - Rasmussen, Søren T

AU - Mouatt, Joshua Roger

AU - Christiansen, Mette Landau Brabæk

AU - Jensen, Bente Rona

AU - Richter, Erik

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AB - In mice, certain proteins show a highly confined expression in specific muscle groups. Also, resting and exercise/contraction-induced phosphorylation responses are higher in rat skeletal muscle with low mitochondrial content compared to muscles with high mitochondrial content, possibly related to differential reactive oxygen species (ROS)-scavenging ability or resting glycogen content. To evaluate these parameters in humans, biopsies from soleus, gastrocnemius and vastus lateralis muscles were taken before and after a 45 min inclined (15%) walking exercise bout at 69% VO2(max) aimed at simultaneously activating soleus and gastrocnemius in a comparable dynamic work-pattern. Hexokinase II and GLUT4 were 46-59% and 26-38% higher (p

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KW - Enzyme Activation

KW - Exercise

KW - Glucose Transporter Type 4

KW - Hexokinase

KW - Humans

KW - Muscle, Skeletal

KW - Phosphorylation

KW - Protein Kinases

KW - Signal Transduction

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EMG-normalised kinase activation during exercise is higher in human gastrocnemius compared to soleus muscle

Abstract

Keywords

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