Enterococcus faecalis clones in poultry and in humans with urinary tract infections, Vietnam

Louise Ladefoged Poulsen; Magne Bisgaard; Nguyen Thai Son; Nguyen Vu Trung; Hoang Manh An; Anders Dalsgaard

doi:10.3201/eid1807.111754

Enterococcus faecalis clones in poultry and in humans with urinary tract infections, Vietnam

Louise Ladefoged Poulsen, Magne Bisgaard, Nguyen Thai Son, Nguyen Vu Trung, Hoang Manh An, Anders Dalsgaard

28 Citations (Scopus)

Abstract

Enterococcus spp. as pathogens have increased, but the sources of infection often remain unclear. To investigate whether poultry might be a reservoir for E. faecalis-associated urinary tract infections (UTIs) in humans, we characterized E. faecalis isolates from patients in Vietnam with UTIs during January 2008-January 2010 and poultry living in close contact with them by multilocus sequence typing (MLST), pulsed-field gel electrophoresis, analysis of antimicrobial drug susceptibility patterns, and sequencing of virulence genes. In 7 (23%) of 31 UTI cases, we detected identical MLST, indistinguishable or closely related pulsed-field gel electrophoresis patterns, and similar antimicrobial drug susceptibility patterns. Isolates from urine and poultry showed identical virulence gene profiles, except for 1 variation, and individual genes showed identical sequences. The homology of isolates from urine and poultry further indicates the zoonotic potential and global spread of E. faecalis sequence type 16, which recently was reported in humans with endocarditis and in pigs in Denmark.

Original language	English
Journal	Emerging Infectious Diseases (Online)
Volume	18
Issue number	7
Pages (from-to)	1096-1100
Number of pages	5
ISSN	1080-6059
DOIs	https://doi.org/10.3201/eid1807.111754
Publication status	Published - Jul 2012

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title = "Enterococcus faecalis clones in poultry and in humans with urinary tract infections, Vietnam",

abstract = "Enterococcus spp. as pathogens have increased, but the sources of infection often remain unclear. To investigate whether poultry might be a reservoir for E. faecalis-associated urinary tract infections (UTIs) in humans, we characterized E. faecalis isolates from patients in Vietnam with UTIs during January 2008-January 2010 and poultry living in close contact with them by multilocus sequence typing (MLST), pulsed-field gel electrophoresis, analysis of antimicrobial drug susceptibility patterns, and sequencing of virulence genes. In 7 (23%) of 31 UTI cases, we detected identical MLST, indistinguishable or closely related pulsed-field gel electrophoresis patterns, and similar antimicrobial drug susceptibility patterns. Isolates from urine and poultry showed identical virulence gene profiles, except for 1 variation, and individual genes showed identical sequences. The homology of isolates from urine and poultry further indicates the zoonotic potential and global spread of E. faecalis sequence type 16, which recently was reported in humans with endocarditis and in pigs in Denmark.",

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T1 - Enterococcus faecalis clones in poultry and in humans with urinary tract infections, Vietnam

AU - Poulsen, Louise Ladefoged

AU - Bisgaard, Magne

AU - Son, Nguyen Thai

AU - Trung, Nguyen Vu

AU - An, Hoang Manh

AU - Dalsgaard, Anders

PY - 2012/7

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AB - Enterococcus spp. as pathogens have increased, but the sources of infection often remain unclear. To investigate whether poultry might be a reservoir for E. faecalis-associated urinary tract infections (UTIs) in humans, we characterized E. faecalis isolates from patients in Vietnam with UTIs during January 2008-January 2010 and poultry living in close contact with them by multilocus sequence typing (MLST), pulsed-field gel electrophoresis, analysis of antimicrobial drug susceptibility patterns, and sequencing of virulence genes. In 7 (23%) of 31 UTI cases, we detected identical MLST, indistinguishable or closely related pulsed-field gel electrophoresis patterns, and similar antimicrobial drug susceptibility patterns. Isolates from urine and poultry showed identical virulence gene profiles, except for 1 variation, and individual genes showed identical sequences. The homology of isolates from urine and poultry further indicates the zoonotic potential and global spread of E. faecalis sequence type 16, which recently was reported in humans with endocarditis and in pigs in Denmark.

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Enterococcus faecalis clones in poultry and in humans with urinary tract infections, Vietnam

Abstract

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