Embryonic expression of Drosophila IMP in the developing CNS and PNS

Sidsel Kramshøj Adolph; Robert Delotto; Finn Cilius Nielsen; Jan Christiansen

doi:10.1016/j.gep.2008.12.001

Embryonic expression of Drosophila IMP in the developing CNS and PNS

Sidsel Kramshøj Adolph, Robert Delotto, Finn Cilius Nielsen, Jan Christiansen

Computational and RNA Biology

10 Citations (Scopus)

Abstract

Drosophila IMP (dIMP) is related to the vertebrate RNA-binding proteins IMP1-3, ZBP1, Vg1RBP and CRD-BP, which are involved in RNA regulatory processes such as translational repression, localization and stabilization. The proteins are expressed in many fetal tissues, including the developing nervous system, and IMP up-regulation in solid tumors correlates with a high metastatic potential and poor prognosis. In this study, we used immunohistochemistry and live-imaging of an endogenous promoter-driven GFP-dIMP fusion protein to reveal the expression pattern of dIMP protein throughout embryogenesis. In the cellular blastoderm, immunoreactivity was seen in the entire cell-layer, where it was localized apically to the nucleus, and in the pole cells. Later, the GFP-dIMP fusion protein appeared in the developing central nervous system, both in the brain and in the ventral nerve cord. In the peripheral nervous system, immunoreactivity was detected in both neurons and accessory cells of chordotonal and external sensory organs
Udgivelsesdato: 2009/3

Original language	English
Journal	Gene Expression Patterns
Volume	9
Issue number	3
Pages (from-to)	138-143
Number of pages	5
ISSN	1567-133X
DOIs	https://doi.org/10.1016/j.gep.2008.12.001
Publication status	Published - 2008

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10.1016/j.gep.2008.12.001

Cite this

@article{e5d82f10e62d11ddbf70000ea68e967b,

title = "Embryonic expression of Drosophila IMP in the developing CNS and PNS",

abstract = "Drosophila IMP (dIMP) is related to the vertebrate RNA-binding proteins IMP1-3, ZBP1, Vg1RBP and CRD-BP, which are involved in RNA regulatory processes such as translational repression, localization and stabilization. The proteins are expressed in many fetal tissues, including the developing nervous system, and IMP up-regulation in solid tumors correlates with a high metastatic potential and poor prognosis. In this study, we used immunohistochemistry and live-imaging of an endogenous promoter-driven GFP-dIMP fusion protein to reveal the expression pattern of dIMP protein throughout embryogenesis. In the cellular blastoderm, immunoreactivity was seen in the entire cell-layer, where it was localized apically to the nucleus, and in the pole cells. Later, the GFP-dIMP fusion protein appeared in the developing central nervous system, both in the brain and in the ventral nerve cord. In the peripheral nervous system, immunoreactivity was detected in both neurons and accessory cells of chordotonal and external sensory organs Udgivelsesdato: 2009/3",

author = "Adolph, {Sidsel Kramsh{\o}j} and Robert Delotto and Nielsen, {Finn Cilius} and Jan Christiansen",

note = "Keywords: IMP; dIMP; ZBP1; Vg1RBP; IGF-II; {\ss}-Actin; CNS; PNS; Sensory organs; Chordotonal organ; External sensory organ; Scolopale cell; Sheath cell; Drosophila; RNA-binding protein",

year = "2008",

doi = "10.1016/j.gep.2008.12.001",

language = "English",

volume = "9",

pages = "138--143",

journal = "Gene Expression Patterns",

issn = "1567-133X",

publisher = "Elsevier",

number = "3",

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TY - JOUR

T1 - Embryonic expression of Drosophila IMP in the developing CNS and PNS

AU - Adolph, Sidsel Kramshøj

AU - Delotto, Robert

AU - Nielsen, Finn Cilius

AU - Christiansen, Jan

N1 - Keywords: IMP; dIMP; ZBP1; Vg1RBP; IGF-II; ß-Actin; CNS; PNS; Sensory organs; Chordotonal organ; External sensory organ; Scolopale cell; Sheath cell; Drosophila; RNA-binding protein

PY - 2008

Y1 - 2008

N2 - Drosophila IMP (dIMP) is related to the vertebrate RNA-binding proteins IMP1-3, ZBP1, Vg1RBP and CRD-BP, which are involved in RNA regulatory processes such as translational repression, localization and stabilization. The proteins are expressed in many fetal tissues, including the developing nervous system, and IMP up-regulation in solid tumors correlates with a high metastatic potential and poor prognosis. In this study, we used immunohistochemistry and live-imaging of an endogenous promoter-driven GFP-dIMP fusion protein to reveal the expression pattern of dIMP protein throughout embryogenesis. In the cellular blastoderm, immunoreactivity was seen in the entire cell-layer, where it was localized apically to the nucleus, and in the pole cells. Later, the GFP-dIMP fusion protein appeared in the developing central nervous system, both in the brain and in the ventral nerve cord. In the peripheral nervous system, immunoreactivity was detected in both neurons and accessory cells of chordotonal and external sensory organs Udgivelsesdato: 2009/3

AB - Drosophila IMP (dIMP) is related to the vertebrate RNA-binding proteins IMP1-3, ZBP1, Vg1RBP and CRD-BP, which are involved in RNA regulatory processes such as translational repression, localization and stabilization. The proteins are expressed in many fetal tissues, including the developing nervous system, and IMP up-regulation in solid tumors correlates with a high metastatic potential and poor prognosis. In this study, we used immunohistochemistry and live-imaging of an endogenous promoter-driven GFP-dIMP fusion protein to reveal the expression pattern of dIMP protein throughout embryogenesis. In the cellular blastoderm, immunoreactivity was seen in the entire cell-layer, where it was localized apically to the nucleus, and in the pole cells. Later, the GFP-dIMP fusion protein appeared in the developing central nervous system, both in the brain and in the ventral nerve cord. In the peripheral nervous system, immunoreactivity was detected in both neurons and accessory cells of chordotonal and external sensory organs Udgivelsesdato: 2009/3

U2 - 10.1016/j.gep.2008.12.001

DO - 10.1016/j.gep.2008.12.001

M3 - Journal article

C2 - 19111951

SN - 1567-133X

VL - 9

SP - 138

EP - 143

JO - Gene Expression Patterns

JF - Gene Expression Patterns

IS - 3

ER -