Alkalilactibacillus ikkensis, gen. nov., sp. nov., a novel enzyme-producing bacterium from a cold and alkaline environment in Greenland

TY - JOUR

T1 - Alkalilactibacillus ikkensis, gen. nov., sp. nov., a novel enzyme-producing bacterium from a cold and alkaline environment in Greenland

AU - Schmidt, Mariane

AU - Priemé, Anders

AU - Johansen, Anders

AU - Stougaard, Peter

PY - 2012/3

Y1 - 2012/3

N2 - Three novel Gram-positive, endospore-forming bacteria were isolated from a cold and alkaline environment. Phylogenetic analysis showed that the strains were almost identical, and that they were related to Natronobacillus azotifigens 24KS-1 T (95. 8% identity), Paraliobacillus quinghaiensis YIM-C158 T (95. 1%), Paraliobacillus ryukyuensis O15-7 T (94. 5%), and Halolactibacillus miurensis M23-1 T (93. 9%). The isolates produced amylase, α-galactosidase, β-galactosidase, and β-glucuronidase, and showed optimal growth at pH 10, at 20°C, and at 2-8% (w/v) NaCl. Major fatty acids were C 14:0 (10. 6-11. 6%), anteiso-C 15:0 (25. 7-32. 7%), C 16:1ω11c (12. 2-16. 0%), and C 16:0 (14. 0-20. 4%). The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and meso-diaminopimelic acid was found in the cell-wall peptidoglycan. The G+C content was 38. 4%. DNA-DNA hybridization between strain GCM68 T and H. miurensis M23-1 T was 32. 4%, while hybridization to N. azotifigens 24KS-1 T, Amphibacillus tropicus Z-7792 T, and Paraliobacillus ryukyuensis O15-7 T was below 30%. The phylogenetic analysis and G+C content place strain GCM68 T in relation to species belonging to Bacillus rRNA group 1, but phylogenetic and physiologic data combined with chemotaxonomic analyses support our proposal for a new genus, Alkalilactibacillus, gen. nov., with the novel species Alkalilactibacillus ikkensis, sp. nov. (type strain is GCM68 T = DSM 19937 = LMG 24405).

AB - Three novel Gram-positive, endospore-forming bacteria were isolated from a cold and alkaline environment. Phylogenetic analysis showed that the strains were almost identical, and that they were related to Natronobacillus azotifigens 24KS-1 T (95. 8% identity), Paraliobacillus quinghaiensis YIM-C158 T (95. 1%), Paraliobacillus ryukyuensis O15-7 T (94. 5%), and Halolactibacillus miurensis M23-1 T (93. 9%). The isolates produced amylase, α-galactosidase, β-galactosidase, and β-glucuronidase, and showed optimal growth at pH 10, at 20°C, and at 2-8% (w/v) NaCl. Major fatty acids were C 14:0 (10. 6-11. 6%), anteiso-C 15:0 (25. 7-32. 7%), C 16:1ω11c (12. 2-16. 0%), and C 16:0 (14. 0-20. 4%). The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and meso-diaminopimelic acid was found in the cell-wall peptidoglycan. The G+C content was 38. 4%. DNA-DNA hybridization between strain GCM68 T and H. miurensis M23-1 T was 32. 4%, while hybridization to N. azotifigens 24KS-1 T, Amphibacillus tropicus Z-7792 T, and Paraliobacillus ryukyuensis O15-7 T was below 30%. The phylogenetic analysis and G+C content place strain GCM68 T in relation to species belonging to Bacillus rRNA group 1, but phylogenetic and physiologic data combined with chemotaxonomic analyses support our proposal for a new genus, Alkalilactibacillus, gen. nov., with the novel species Alkalilactibacillus ikkensis, sp. nov. (type strain is GCM68 T = DSM 19937 = LMG 24405).

U2 - 10.1007/s00792-012-0430-7

DO - 10.1007/s00792-012-0430-7

M3 - Journal article

C2 - 22297696

SN - 1431-0651

VL - 16

SP - 297

EP - 305

JO - Extremophiles

JF - Extremophiles

IS - 2

ER -