TY - JOUR
T1 - β2-agonist induces net leg glucose uptake and free fatty acid release at rest but not during exercise in young men
AU - Onslev, Johan
AU - Jensen, Jørgen
AU - Bangsbo, Jens
AU - Wojtaszewski, Jørgen
AU - Hostrup, Morten
N1 - CURIS 2019 NEXS 029
PY - 2018/12/28
Y1 - 2018/12/28
N2 - Objective The role of selective β 2-Adrenergic stimulation in regulation of leg glucose uptake and free fatty acid (FFA) balance is inadequately explored in humans. The objective of this study was to investigate β 2-Adrenergic effects on net leg glucose uptake and clearance, as well as FFA balance at rest and during exercise. Design The study was a randomized, placebo-controlled crossover trial where 10 healthy men received either infusion of β 2-Agonist terbutaline (0.2 to 0.4 mg) or placebo. Net leg glucose uptake and clearance and FFA balance were determined at rest and during 8 minutes of knee extensor exercise using Fick's principle. Vastus lateralis muscle biopsies were collected at rest and at cessation of exercise. The primary outcome measure was net leg glucose uptake. Results At rest, net leg glucose uptake and clearance were 0.35 (±0.16) mmol/min and 41 (±17) mL/min (mean ± 95% CI) higher (P < 0.001) for terbutaline than placebo, corresponding to increases of 84% and 70%. During exercise, no treatment differences were observed in net leg glucose uptake, whereas clearance was 101 (±86) mL/min lower (P < 0.05) for terbutaline than placebo. At rest, terbutaline induced a net leg FFA release of 21 (±14) μmol/min, being different from placebo (P = 0.04). During exercise, net leg FFA uptake was not different between the treatments. Conclusions These observations indicate that β 2-Agonist alters net leg glucose uptake and clearance, as well as FFA balance in humans, which is associated with myocellular β 2-Adrenergic and insulin-dependent signaling. Furthermore, the study shows that exercise confounds the β 2-Adrenergic effect on net leg glucose uptake and FFA balance.
AB - Objective The role of selective β 2-Adrenergic stimulation in regulation of leg glucose uptake and free fatty acid (FFA) balance is inadequately explored in humans. The objective of this study was to investigate β 2-Adrenergic effects on net leg glucose uptake and clearance, as well as FFA balance at rest and during exercise. Design The study was a randomized, placebo-controlled crossover trial where 10 healthy men received either infusion of β 2-Agonist terbutaline (0.2 to 0.4 mg) or placebo. Net leg glucose uptake and clearance and FFA balance were determined at rest and during 8 minutes of knee extensor exercise using Fick's principle. Vastus lateralis muscle biopsies were collected at rest and at cessation of exercise. The primary outcome measure was net leg glucose uptake. Results At rest, net leg glucose uptake and clearance were 0.35 (±0.16) mmol/min and 41 (±17) mL/min (mean ± 95% CI) higher (P < 0.001) for terbutaline than placebo, corresponding to increases of 84% and 70%. During exercise, no treatment differences were observed in net leg glucose uptake, whereas clearance was 101 (±86) mL/min lower (P < 0.05) for terbutaline than placebo. At rest, terbutaline induced a net leg FFA release of 21 (±14) μmol/min, being different from placebo (P = 0.04). During exercise, net leg FFA uptake was not different between the treatments. Conclusions These observations indicate that β 2-Agonist alters net leg glucose uptake and clearance, as well as FFA balance in humans, which is associated with myocellular β 2-Adrenergic and insulin-dependent signaling. Furthermore, the study shows that exercise confounds the β 2-Adrenergic effect on net leg glucose uptake and FFA balance.
U2 - 10.1210/jc.2018-01349
DO - 10.1210/jc.2018-01349
M3 - Journal article
C2 - 30285125
SN - 0021-972X
VL - 104
SP - 647
EP - 657
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 3
ER -