TY - JOUR
T1 - Electrokinetic migration across artificial liquid membranes. Tuning the membrane chemistry to different types of drug substances
AU - Gjelstad, Astrid
AU - Rasmussen, Knut Einar
AU - Pedersen-Bjergaard, Stig
PY - 2006/8/18
Y1 - 2006/8/18
N2 - Twenty different basic drugs were electrokinetically extracted across a thin artificial organic liquid membrane with a 300 V d.c. electrical potential difference as the driving force. From a 300 μl aqueous sample (acidified corresponding to 10 mM HCl), the drugs were extracted for 5 min through a 200 μm artificial liquid membrane of a water immiscible organic solvent immobilized in the pores of a polypropylene hollow fiber, and into a 30 μl aqueous acceptor solution of 10 mM HCl inside the lumen of the hollow fiber. Hydrophobic basic drugs (log P > 1.7) were effectively isolated utilizing 2-nitrophenyl octyl ether (NPOE) as the artificial liquid membrane, with recoveries up to 83%. For more hydrophilic basic drugs (log P < 1.0), a mixture of NPOE and 25% (w/w) di-(2-ethylhexyl) phosphate (DEHP) was required to ensure efficient extraction, resulting in recoveries up to 75%. DEHP was expected to act as an ion-pair reagent ion-pairing the protonated hydrophilic drugs at the interface between the sample and the membrane, resulting in permeation of the interface.
AB - Twenty different basic drugs were electrokinetically extracted across a thin artificial organic liquid membrane with a 300 V d.c. electrical potential difference as the driving force. From a 300 μl aqueous sample (acidified corresponding to 10 mM HCl), the drugs were extracted for 5 min through a 200 μm artificial liquid membrane of a water immiscible organic solvent immobilized in the pores of a polypropylene hollow fiber, and into a 30 μl aqueous acceptor solution of 10 mM HCl inside the lumen of the hollow fiber. Hydrophobic basic drugs (log P > 1.7) were effectively isolated utilizing 2-nitrophenyl octyl ether (NPOE) as the artificial liquid membrane, with recoveries up to 83%. For more hydrophilic basic drugs (log P < 1.0), a mixture of NPOE and 25% (w/w) di-(2-ethylhexyl) phosphate (DEHP) was required to ensure efficient extraction, resulting in recoveries up to 75%. DEHP was expected to act as an ion-pair reagent ion-pairing the protonated hydrophilic drugs at the interface between the sample and the membrane, resulting in permeation of the interface.
KW - Artificial liquid membranes
KW - Drugs
KW - Electrokinetic migration
KW - Selectivity
UR - http://www.scopus.com/inward/record.url?scp=33746504559&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2006.04.039
DO - 10.1016/j.chroma.2006.04.039
M3 - Journal article
C2 - 16696986
AN - SCOPUS:33746504559
SN - 0301-4770
VL - 1124
SP - 29
EP - 34
JO - Journal of Chromatography
JF - Journal of Chromatography
IS - 1-2
ER -