Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples

Linda Vårdal; Astrid Gjelstad; Chuixiu Huang; Elisabeth Leere Øiestad; Stig Pedersen-Bjergaard

doi:10.4155/bio-2017-0027

Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples

Linda Vårdal, Astrid Gjelstad, Chuixiu Huang, Elisabeth Leere Øiestad, Stig Pedersen-Bjergaard

14 Citations (Scopus)

Abstract

Aim: For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems. Results & discussion: No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts. Conclusion: Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.

Original language	English
Journal	Bioanalysis
Volume	9
Issue number	8
Pages (from-to)	631-641
Number of pages	11
ISSN	1757-6180
DOIs	https://doi.org/10.4155/bio-2017-0027
Publication status	Published - Apr 2017

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title = "Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples",

abstract = "Aim: For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems. Results & discussion: No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts. Conclusion: Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.",

author = "Linda V{\aa}rdal and Astrid Gjelstad and Chuixiu Huang and {\O}iestad, {Elisabeth Leere} and Stig Pedersen-Bjergaard",

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doi = "10.4155/bio-2017-0027",

language = "English",

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T1 - Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples

AU - Vårdal, Linda

AU - Gjelstad, Astrid

AU - Huang, Chuixiu

AU - Øiestad, Elisabeth Leere

AU - Pedersen-Bjergaard, Stig

PY - 2017/4

Y1 - 2017/4

N2 - Aim: For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems. Results & discussion: No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts. Conclusion: Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.

AB - Aim: For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems. Results & discussion: No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts. Conclusion: Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.

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DO - 10.4155/bio-2017-0027

M3 - Journal article

C2 - 28508669

SN - 1757-6180

VL - 9

SP - 631

EP - 641

JO - Bioanalysis

JF - Bioanalysis

IS - 8

ER -

Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples

Abstract

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