Effects of whole-body heat acclimation on cell injury and cytokine responses in peripheral blood mononuclear cells

TY - JOUR

T1 - Effects of whole-body heat acclimation on cell injury and cytokine responses in peripheral blood mononuclear cells

AU - Amorim, Fabiano

AU - Yamada, Paulette

AU - Robergs, Robert

AU - Schneider, Suzanne

AU - Moseley, Pope

PY - 2011/8

Y1 - 2011/8

N2 - To test the hypothesis that whole-body heat acclimation (HA) would increase peripheral blood mononuclear cells' (PBMC) tolerance to heat shock (HS) and/or alter the release of cytokines (IL-1β, IL-6, IL-10 and TNFa) to bacterial lipopolysaccharide (LPS), we heat acclimated nine subjects by exercising them for 100 min in a hot environment for 10 days. The subjects' PBMC were separated and cultured on days 1 and 10 of HA pre- and post-exercise. Pre-exercise PBMC were allocated to three treatments: control (PRE, 37°C), HS (42.5°C for 2 h), or LPS (1 ng mL -1 for 24 h). Post-exercise samples were incubated at 37°C. PBMC lactate dehydrogenase release increased (p<0.05) after HS but it was not different (p>0.05) between days 1 and 10 (0.100 ± 0.012 and 0.102 ± 0.16 abs., respectively). LPS treatment induced an increased (p<0.05) release of cytokines but HA did not alter this response (p[0.05). Pre-exercise intracellular heat shock protein 72 (Hsp72) was higher (p<0.05) on day 10 compared to day 1 of HA (13 ± 5 and 8 ± 5 ng mL -1, respectively). HS treatment caused a greater increase (p\0.05) in Hsp72 than the exercise sessions on HA days 1 and 10. In addition, after HA, the Hsp72 response to HS was reduced (day 1, 129 ± 46; day 10, 80 ± 32 ng mL -1, p<0.05). In conclusion, HA increases PBMC Hsp72 but it does not reduce cellular damage to HS or alter cytokine response to LPS. We speculate that the stress applied during HA is not sufficient to modify the PBMC response.

AB - To test the hypothesis that whole-body heat acclimation (HA) would increase peripheral blood mononuclear cells' (PBMC) tolerance to heat shock (HS) and/or alter the release of cytokines (IL-1β, IL-6, IL-10 and TNFa) to bacterial lipopolysaccharide (LPS), we heat acclimated nine subjects by exercising them for 100 min in a hot environment for 10 days. The subjects' PBMC were separated and cultured on days 1 and 10 of HA pre- and post-exercise. Pre-exercise PBMC were allocated to three treatments: control (PRE, 37°C), HS (42.5°C for 2 h), or LPS (1 ng mL -1 for 24 h). Post-exercise samples were incubated at 37°C. PBMC lactate dehydrogenase release increased (p<0.05) after HS but it was not different (p>0.05) between days 1 and 10 (0.100 ± 0.012 and 0.102 ± 0.16 abs., respectively). LPS treatment induced an increased (p<0.05) release of cytokines but HA did not alter this response (p[0.05). Pre-exercise intracellular heat shock protein 72 (Hsp72) was higher (p<0.05) on day 10 compared to day 1 of HA (13 ± 5 and 8 ± 5 ng mL -1, respectively). HS treatment caused a greater increase (p\0.05) in Hsp72 than the exercise sessions on HA days 1 and 10. In addition, after HA, the Hsp72 response to HS was reduced (day 1, 129 ± 46; day 10, 80 ± 32 ng mL -1, p<0.05). In conclusion, HA increases PBMC Hsp72 but it does not reduce cellular damage to HS or alter cytokine response to LPS. We speculate that the stress applied during HA is not sufficient to modify the PBMC response.

KW - Acclimatization/physiology

KW - Adult

KW - Body Temperature Regulation/physiology

KW - Cells, Cultured

KW - Cytokines/metabolism

KW - Environment, Controlled

KW - Exercise/physiology

KW - Exercise Test

KW - Female

KW - Heat-Shock Response/physiology

KW - Hot Temperature

KW - Humans

KW - Leukocytes, Mononuclear/metabolism

KW - Male

KW - Young Adult

U2 - 10.1007/s00421-010-1780-4

DO - 10.1007/s00421-010-1780-4

M3 - Journal article

C2 - 21191798

SN - 1439-6319

VL - 111

SP - 1609

EP - 1618

JO - European Journal of Applied Physiology

JF - European Journal of Applied Physiology

IS - 8

ER -