Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri

Jakob Skov; Jiwan K. Chettri; Rzgar M. Jaafar; Per W. Kania; Inger Dalsgaard; Kurt Buchmann

doi:10.1016/j.aquaculture.2018.04.011

Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri

Jakob Skov, Jiwan K. Chettri, Rzgar M. Jaafar, Per W. Kania, Inger Dalsgaard, Kurt Buchmann^*

^*Corresponding author for this work

14 Citations (Scopus)

Abstract

Immersion vaccination of rainbow trout against Yersinia ruckeri infection is an established method to prevent enteric red mouth disease (ERM) but the effect is inferior to injection vaccination and the duration of protection is limited to less than six months. Adjuvants in vaccines may in general elevate the immune response and the present work elucidates how ERM immersion vaccination of trout in combination with exposure to soluble adjuvants, Montanide™ IMS 1312 VG PR and β-glucan, affects immune reactions. The former adjuvant, when used alone, induced a slightly increased protection (not statistically significant) whereas β-glucan did not increase protection. Adjuvant-treated and non-exposed groups showed elevated plasma lysozyme activity after challenge with Y. ruckeri. Specific antibody production was not positively affected by combining adjuvant and vaccine. Overall expression of immune genes tested was generally manifold higher in gills compared to skin. Only genes encoding SAA and IL-17C1 were expressed at a higher level in skin. Dynamic differences between the gill and skin compartments were also recorded for genes encoding cytokines (TNF-α, IL-1β, IL-6, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22), immunoglobulins (IgM, IgD, IgT), cell markers (CD8α, TCR-β) and acute phase reactants (SAA, lysozyme). These genes were upregulated 24 h post-vaccination in fish gills exposed to both vaccine-adjuvant combinations when compared to fish exposed to vaccine alone. After a few weeks no vaccine induced reaction was seen and after challenge with bacteria mainly unvaccinated fish responded. Adjuvants used in combination with immersion vaccine clearly influences immune reactions and may improve duration and protection but further potency tests should be performed.

Original language	English
Journal	Aquaculture
Volume	492
Pages (from-to)	237-246
Number of pages	10
ISSN	0044-8486
DOIs	https://doi.org/10.1016/j.aquaculture.2018.04.011
Publication status	Published - 1 Jul 2018

Keywords

Adjuvant
Immersion vaccination
Montanide™
Yersinia ruckeri
β-glucan

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.aquaculture.2018.04.011

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title = "Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri",

abstract = "Immersion vaccination of rainbow trout against Yersinia ruckeri infection is an established method to prevent enteric red mouth disease (ERM) but the effect is inferior to injection vaccination and the duration of protection is limited to less than six months. Adjuvants in vaccines may in general elevate the immune response and the present work elucidates how ERM immersion vaccination of trout in combination with exposure to soluble adjuvants, Montanide{\texttrademark} IMS 1312 VG PR and β-glucan, affects immune reactions. The former adjuvant, when used alone, induced a slightly increased protection (not statistically significant) whereas β-glucan did not increase protection. Adjuvant-treated and non-exposed groups showed elevated plasma lysozyme activity after challenge with Y. ruckeri. Specific antibody production was not positively affected by combining adjuvant and vaccine. Overall expression of immune genes tested was generally manifold higher in gills compared to skin. Only genes encoding SAA and IL-17C1 were expressed at a higher level in skin. Dynamic differences between the gill and skin compartments were also recorded for genes encoding cytokines (TNF-α, IL-1β, IL-6, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22), immunoglobulins (IgM, IgD, IgT), cell markers (CD8α, TCR-β) and acute phase reactants (SAA, lysozyme). These genes were upregulated 24 h post-vaccination in fish gills exposed to both vaccine-adjuvant combinations when compared to fish exposed to vaccine alone. After a few weeks no vaccine induced reaction was seen and after challenge with bacteria mainly unvaccinated fish responded. Adjuvants used in combination with immersion vaccine clearly influences immune reactions and may improve duration and protection but further potency tests should be performed.",

keywords = "Adjuvant, Immersion vaccination, Montanide{\texttrademark}, Yersinia ruckeri, β-glucan",

author = "Jakob Skov and Chettri, {Jiwan K.} and Jaafar, {Rzgar M.} and Kania, {Per W.} and Inger Dalsgaard and Kurt Buchmann",

year = "2018",

month = jul,

day = "1",

doi = "10.1016/j.aquaculture.2018.04.011",

language = "English",

volume = "492",

pages = "237--246",

journal = "Aquaculture",

issn = "0044-8486",

publisher = "Elsevier",

}

TY - JOUR

T1 - Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri

AU - Skov, Jakob

AU - Chettri, Jiwan K.

AU - Jaafar, Rzgar M.

AU - Kania, Per W.

AU - Dalsgaard, Inger

AU - Buchmann, Kurt

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Immersion vaccination of rainbow trout against Yersinia ruckeri infection is an established method to prevent enteric red mouth disease (ERM) but the effect is inferior to injection vaccination and the duration of protection is limited to less than six months. Adjuvants in vaccines may in general elevate the immune response and the present work elucidates how ERM immersion vaccination of trout in combination with exposure to soluble adjuvants, Montanide™ IMS 1312 VG PR and β-glucan, affects immune reactions. The former adjuvant, when used alone, induced a slightly increased protection (not statistically significant) whereas β-glucan did not increase protection. Adjuvant-treated and non-exposed groups showed elevated plasma lysozyme activity after challenge with Y. ruckeri. Specific antibody production was not positively affected by combining adjuvant and vaccine. Overall expression of immune genes tested was generally manifold higher in gills compared to skin. Only genes encoding SAA and IL-17C1 were expressed at a higher level in skin. Dynamic differences between the gill and skin compartments were also recorded for genes encoding cytokines (TNF-α, IL-1β, IL-6, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22), immunoglobulins (IgM, IgD, IgT), cell markers (CD8α, TCR-β) and acute phase reactants (SAA, lysozyme). These genes were upregulated 24 h post-vaccination in fish gills exposed to both vaccine-adjuvant combinations when compared to fish exposed to vaccine alone. After a few weeks no vaccine induced reaction was seen and after challenge with bacteria mainly unvaccinated fish responded. Adjuvants used in combination with immersion vaccine clearly influences immune reactions and may improve duration and protection but further potency tests should be performed.

AB - Immersion vaccination of rainbow trout against Yersinia ruckeri infection is an established method to prevent enteric red mouth disease (ERM) but the effect is inferior to injection vaccination and the duration of protection is limited to less than six months. Adjuvants in vaccines may in general elevate the immune response and the present work elucidates how ERM immersion vaccination of trout in combination with exposure to soluble adjuvants, Montanide™ IMS 1312 VG PR and β-glucan, affects immune reactions. The former adjuvant, when used alone, induced a slightly increased protection (not statistically significant) whereas β-glucan did not increase protection. Adjuvant-treated and non-exposed groups showed elevated plasma lysozyme activity after challenge with Y. ruckeri. Specific antibody production was not positively affected by combining adjuvant and vaccine. Overall expression of immune genes tested was generally manifold higher in gills compared to skin. Only genes encoding SAA and IL-17C1 were expressed at a higher level in skin. Dynamic differences between the gill and skin compartments were also recorded for genes encoding cytokines (TNF-α, IL-1β, IL-6, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22), immunoglobulins (IgM, IgD, IgT), cell markers (CD8α, TCR-β) and acute phase reactants (SAA, lysozyme). These genes were upregulated 24 h post-vaccination in fish gills exposed to both vaccine-adjuvant combinations when compared to fish exposed to vaccine alone. After a few weeks no vaccine induced reaction was seen and after challenge with bacteria mainly unvaccinated fish responded. Adjuvants used in combination with immersion vaccine clearly influences immune reactions and may improve duration and protection but further potency tests should be performed.

KW - Adjuvant

KW - Immersion vaccination

KW - Montanide™

KW - Yersinia ruckeri

KW - β-glucan

U2 - 10.1016/j.aquaculture.2018.04.011

DO - 10.1016/j.aquaculture.2018.04.011

M3 - Journal article

AN - SCOPUS:85045551526

SN - 0044-8486

VL - 492

SP - 237

EP - 246

JO - Aquaculture

JF - Aquaculture

ER -

Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeri

Abstract

Keywords

UN SDGs

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