Effects of oxidation on the physicochemical properties of polyunsaturated lipid membranes

Elisa Parra; Kathryn Browning; Liv Sofia Elinor Damgaard; Randi Nordström; Samantha Micciulla; Saskia Bucciarelli; Martin Malmsten

doi:10.1016/j.jcis.2018.12.007

Effects of oxidation on the physicochemical properties of polyunsaturated lipid membranes

Elisa Parra, Kathryn Browning, Liv Sofia Elinor Damgaard, Randi Nordström, Samantha Micciulla, Saskia Bucciarelli, Martin Malmsten

10 Citations (Scopus)

Abstract

The exposure of biological membranes to reactive oxygen species (ROS) plays an important role in many pathological conditions such as inflammation, infection, or sepsis. ROS also modulate signaling processes and produce markers for damaged tissue. Lipid peroxidation, mainly affecting polyunsaturated phospholipids, results in a complex mixture of oxidized products, which may dramatically alter membrane properties. Here, we have employed a set of biophysical and surface-chemical techniques, including neutron and X-ray scattering, to study the structural, compositional, and stability changes due to oxidative stress on phospholipid bilayers composed of lipids with different degrees of polyunsaturation. In doing so, we obtained real-time information about bilayer degradation under in situ UV exposure using neutron reflectometry. We present a set of interrelated physicochemical effects, including gradual increases in area per molecule, head group and acyl chain hydration, as well as bilayer thinning, lateral phase separation, and defect formation leading to content loss upon membrane oxidation. Such effects were observed to depend on the presence of polyunsaturated phospholipids in the lipid membrane, suggesting that these may also play a role in the complex oxidation processes occurring in cells.

Original language	English
Article number	538
Journal	Journal of Colloid and Interface Science
Pages (from-to)	404-419
Number of pages	15
ISSN	0021-9797
DOIs	https://doi.org/10.1016/j.jcis.2018.12.007
Publication status	Published - 7 Mar 2019

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@article{b84144f6ae254d9faac85a50908c3268,

title = "Effects of oxidation on the physicochemical properties of polyunsaturated lipid membranes",

abstract = "The exposure of biological membranes to reactive oxygen species (ROS) plays an important role in many pathological conditions such as inflammation, infection, or sepsis. ROS also modulate signaling processes and produce markers for damaged tissue. Lipid peroxidation, mainly affecting polyunsaturated phospholipids, results in a complex mixture of oxidized products, which may dramatically alter membrane properties. Here, we have employed a set of biophysical and surface-chemical techniques, including neutron and X-ray scattering, to study the structural, compositional, and stability changes due to oxidative stress on phospholipid bilayers composed of lipids with different degrees of polyunsaturation. In doing so, we obtained real-time information about bilayer degradation under in situ UV exposure using neutron reflectometry. We present a set of interrelated physicochemical effects, including gradual increases in area per molecule, head group and acyl chain hydration, as well as bilayer thinning, lateral phase separation, and defect formation leading to content loss upon membrane oxidation. Such effects were observed to depend on the presence of polyunsaturated phospholipids in the lipid membrane, suggesting that these may also play a role in the complex oxidation processes occurring in cells.",

author = "Elisa Parra and Kathryn Browning and Damgaard, {Liv Sofia Elinor} and Randi Nordstr{\"o}m and Samantha Micciulla and Saskia Bucciarelli and Martin Malmsten",

year = "2019",

month = mar,

day = "7",

doi = "10.1016/j.jcis.2018.12.007",

language = "English",

pages = "404--419",

journal = "Journal of Colloid and Interface Science",

issn = "0021-9797",

publisher = "Academic Press",

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TY - JOUR

T1 - Effects of oxidation on the physicochemical properties of polyunsaturated lipid membranes

AU - Parra, Elisa

AU - Browning, Kathryn

AU - Damgaard, Liv Sofia Elinor

AU - Nordström, Randi

AU - Micciulla, Samantha

AU - Bucciarelli, Saskia

AU - Malmsten, Martin

PY - 2019/3/7

Y1 - 2019/3/7

N2 - The exposure of biological membranes to reactive oxygen species (ROS) plays an important role in many pathological conditions such as inflammation, infection, or sepsis. ROS also modulate signaling processes and produce markers for damaged tissue. Lipid peroxidation, mainly affecting polyunsaturated phospholipids, results in a complex mixture of oxidized products, which may dramatically alter membrane properties. Here, we have employed a set of biophysical and surface-chemical techniques, including neutron and X-ray scattering, to study the structural, compositional, and stability changes due to oxidative stress on phospholipid bilayers composed of lipids with different degrees of polyunsaturation. In doing so, we obtained real-time information about bilayer degradation under in situ UV exposure using neutron reflectometry. We present a set of interrelated physicochemical effects, including gradual increases in area per molecule, head group and acyl chain hydration, as well as bilayer thinning, lateral phase separation, and defect formation leading to content loss upon membrane oxidation. Such effects were observed to depend on the presence of polyunsaturated phospholipids in the lipid membrane, suggesting that these may also play a role in the complex oxidation processes occurring in cells.

AB - The exposure of biological membranes to reactive oxygen species (ROS) plays an important role in many pathological conditions such as inflammation, infection, or sepsis. ROS also modulate signaling processes and produce markers for damaged tissue. Lipid peroxidation, mainly affecting polyunsaturated phospholipids, results in a complex mixture of oxidized products, which may dramatically alter membrane properties. Here, we have employed a set of biophysical and surface-chemical techniques, including neutron and X-ray scattering, to study the structural, compositional, and stability changes due to oxidative stress on phospholipid bilayers composed of lipids with different degrees of polyunsaturation. In doing so, we obtained real-time information about bilayer degradation under in situ UV exposure using neutron reflectometry. We present a set of interrelated physicochemical effects, including gradual increases in area per molecule, head group and acyl chain hydration, as well as bilayer thinning, lateral phase separation, and defect formation leading to content loss upon membrane oxidation. Such effects were observed to depend on the presence of polyunsaturated phospholipids in the lipid membrane, suggesting that these may also play a role in the complex oxidation processes occurring in cells.

U2 - 10.1016/j.jcis.2018.12.007

DO - 10.1016/j.jcis.2018.12.007

M3 - Journal article

SN - 0021-9797

SP - 404

EP - 419

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

M1 - 538

ER -

Effects of oxidation on the physicochemical properties of polyunsaturated lipid membranes

Abstract

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