Effects of long-term storage at -14°C on the survival of Neozygites fresenii (Entomophthorales: Neozygitaceae) in cotton aphids (Homoptera: Aphididae)

Mette G. Vingaard; Donald C. Steinkraus; Gabriele O. Boys; Jørgen Eilenberg

doi:10.1016/S0022-2011(02)00205-7

Effects of long-term storage at -14°C on the survival of Neozygites fresenii (Entomophthorales: Neozygitaceae) in cotton aphids (Homoptera: Aphididae)

Mette G. Vingaard, Donald C. Steinkraus^*, Gabriele O. Boys, Jørgen Eilenberg

^*Corresponding author for this work

Section for Organismal Biology

11 Citations (Scopus)

Abstract

Neozygites fresenii-infected Aphis gossypii cadavers, containing dormant hyphal bodies of N. fresenii, were stored in 4 ml glass vials at -14°C in a standard consumer-type refrigerator/freezer for 1, 21, 30, 43, 51, and 68 months to determine the effect of storage on fungal survival. When the cadavers were removed from the freezer and placed in 25 ± 1°C, 100% relative humidity, and 12:12 (L:D) conditions, N. fresenii survival, as shown by fungal sporulation from the cadavers, was high at all storage periods. The average percentage of cadavers from which the fungus sporulated were 93, 47, 100, 100, 80, and 60% from 1, 21, 30, 43, 51, and 68 months storage periods, respectively. The number of primary conidia discharged from each sporulating cadaver was estimated using a scale of 1 (low, ca. 1000 primary conidia), 2 (medium, ca. 2000 primary conidia) and 3 (high, ca. 3000 primary conidia). The median scores for the number of primary conidia produced per sporulating cadaver were 3, 2, 3, 3, 2.5, and 1 for 1, 21, 30, 43, 51, and 68 months, respectively. Therefore, except for the longest storage period, most cadavers produced medium to high numbers of primary conidia. Mean germination of primary conidia produced from N. fresenii-infected-aphid cadavers from each time period varied significantly from 66.3 to 86.1% in the 21 and 43 months categories, respectively. Infectivity of capilliconidia, produced from frozen N. fresenii, to live healthy cotton aphids varied significantly from 16.7 to 68.7% from cadavers stored 68 months and 1 month, respectively. Overall N. fresenii survived well in dried frozen cotton aphid cadavers for up to 6 years with little reduction in sporulation, numbers of spores produced, germination of primary conidia, or infectivity.

Original language	English
Journal	Journal of Invertebrate Pathology
Volume	82
Issue number	2
Pages (from-to)	97-102
Number of pages	6
ISSN	0022-2011
DOIs	https://doi.org/10.1016/S0022-2011(02)00205-7
Publication status	Published - 1 Feb 2003

Keywords

Aphis gossypii
Biological control
In vivo storage
Infectivity
Neozygites fresenii
Sporulation

Access to Document

10.1016/S0022-2011(02)00205-7

Cite this

Effects of long-term storage at -14°C on the survival of Neozygites fresenii (Entomophthorales : Neozygitaceae) in cotton aphids (Homoptera: Aphididae). / Vingaard, Mette G.; Steinkraus, Donald C.; Boys, Gabriele O. et al.

In: Journal of Invertebrate Pathology, Vol. 82, No. 2, 01.02.2003, p. 97-102.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{17eddf965e904413b740a97e8abfb533,

title = "Effects of long-term storage at -14°C on the survival of Neozygites fresenii (Entomophthorales: Neozygitaceae) in cotton aphids (Homoptera: Aphididae)",

abstract = "Neozygites fresenii-infected Aphis gossypii cadavers, containing dormant hyphal bodies of N. fresenii, were stored in 4 ml glass vials at -14°C in a standard consumer-type refrigerator/freezer for 1, 21, 30, 43, 51, and 68 months to determine the effect of storage on fungal survival. When the cadavers were removed from the freezer and placed in 25 ± 1°C, 100% relative humidity, and 12:12 (L:D) conditions, N. fresenii survival, as shown by fungal sporulation from the cadavers, was high at all storage periods. The average percentage of cadavers from which the fungus sporulated were 93, 47, 100, 100, 80, and 60% from 1, 21, 30, 43, 51, and 68 months storage periods, respectively. The number of primary conidia discharged from each sporulating cadaver was estimated using a scale of 1 (low, ca. 1000 primary conidia), 2 (medium, ca. 2000 primary conidia) and 3 (high, ca. 3000 primary conidia). The median scores for the number of primary conidia produced per sporulating cadaver were 3, 2, 3, 3, 2.5, and 1 for 1, 21, 30, 43, 51, and 68 months, respectively. Therefore, except for the longest storage period, most cadavers produced medium to high numbers of primary conidia. Mean germination of primary conidia produced from N. fresenii-infected-aphid cadavers from each time period varied significantly from 66.3 to 86.1% in the 21 and 43 months categories, respectively. Infectivity of capilliconidia, produced from frozen N. fresenii, to live healthy cotton aphids varied significantly from 16.7 to 68.7% from cadavers stored 68 months and 1 month, respectively. Overall N. fresenii survived well in dried frozen cotton aphid cadavers for up to 6 years with little reduction in sporulation, numbers of spores produced, germination of primary conidia, or infectivity.",

keywords = "Aphis gossypii, Biological control, In vivo storage, Infectivity, Neozygites fresenii, Sporulation",

author = "Vingaard, {Mette G.} and Steinkraus, {Donald C.} and Boys, {Gabriele O.} and J{\o}rgen Eilenberg",

year = "2003",

month = feb,

day = "1",

doi = "10.1016/S0022-2011(02)00205-7",

language = "English",

volume = "82",

pages = "97--102",

journal = "Journal of Invertebrate Pathology",

issn = "0022-2011",

publisher = "Academic Press",

number = "2",

}

TY - JOUR

T1 - Effects of long-term storage at -14°C on the survival of Neozygites fresenii (Entomophthorales

T2 - Neozygitaceae) in cotton aphids (Homoptera: Aphididae)

AU - Vingaard, Mette G.

AU - Steinkraus, Donald C.

AU - Boys, Gabriele O.

AU - Eilenberg, Jørgen

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Neozygites fresenii-infected Aphis gossypii cadavers, containing dormant hyphal bodies of N. fresenii, were stored in 4 ml glass vials at -14°C in a standard consumer-type refrigerator/freezer for 1, 21, 30, 43, 51, and 68 months to determine the effect of storage on fungal survival. When the cadavers were removed from the freezer and placed in 25 ± 1°C, 100% relative humidity, and 12:12 (L:D) conditions, N. fresenii survival, as shown by fungal sporulation from the cadavers, was high at all storage periods. The average percentage of cadavers from which the fungus sporulated were 93, 47, 100, 100, 80, and 60% from 1, 21, 30, 43, 51, and 68 months storage periods, respectively. The number of primary conidia discharged from each sporulating cadaver was estimated using a scale of 1 (low, ca. 1000 primary conidia), 2 (medium, ca. 2000 primary conidia) and 3 (high, ca. 3000 primary conidia). The median scores for the number of primary conidia produced per sporulating cadaver were 3, 2, 3, 3, 2.5, and 1 for 1, 21, 30, 43, 51, and 68 months, respectively. Therefore, except for the longest storage period, most cadavers produced medium to high numbers of primary conidia. Mean germination of primary conidia produced from N. fresenii-infected-aphid cadavers from each time period varied significantly from 66.3 to 86.1% in the 21 and 43 months categories, respectively. Infectivity of capilliconidia, produced from frozen N. fresenii, to live healthy cotton aphids varied significantly from 16.7 to 68.7% from cadavers stored 68 months and 1 month, respectively. Overall N. fresenii survived well in dried frozen cotton aphid cadavers for up to 6 years with little reduction in sporulation, numbers of spores produced, germination of primary conidia, or infectivity.

AB - Neozygites fresenii-infected Aphis gossypii cadavers, containing dormant hyphal bodies of N. fresenii, were stored in 4 ml glass vials at -14°C in a standard consumer-type refrigerator/freezer for 1, 21, 30, 43, 51, and 68 months to determine the effect of storage on fungal survival. When the cadavers were removed from the freezer and placed in 25 ± 1°C, 100% relative humidity, and 12:12 (L:D) conditions, N. fresenii survival, as shown by fungal sporulation from the cadavers, was high at all storage periods. The average percentage of cadavers from which the fungus sporulated were 93, 47, 100, 100, 80, and 60% from 1, 21, 30, 43, 51, and 68 months storage periods, respectively. The number of primary conidia discharged from each sporulating cadaver was estimated using a scale of 1 (low, ca. 1000 primary conidia), 2 (medium, ca. 2000 primary conidia) and 3 (high, ca. 3000 primary conidia). The median scores for the number of primary conidia produced per sporulating cadaver were 3, 2, 3, 3, 2.5, and 1 for 1, 21, 30, 43, 51, and 68 months, respectively. Therefore, except for the longest storage period, most cadavers produced medium to high numbers of primary conidia. Mean germination of primary conidia produced from N. fresenii-infected-aphid cadavers from each time period varied significantly from 66.3 to 86.1% in the 21 and 43 months categories, respectively. Infectivity of capilliconidia, produced from frozen N. fresenii, to live healthy cotton aphids varied significantly from 16.7 to 68.7% from cadavers stored 68 months and 1 month, respectively. Overall N. fresenii survived well in dried frozen cotton aphid cadavers for up to 6 years with little reduction in sporulation, numbers of spores produced, germination of primary conidia, or infectivity.

KW - Aphis gossypii

KW - Biological control

KW - In vivo storage

KW - Infectivity

KW - Neozygites fresenii

KW - Sporulation

UR - http://www.scopus.com/inward/record.url?scp=0037314520&partnerID=8YFLogxK

U2 - 10.1016/S0022-2011(02)00205-7

DO - 10.1016/S0022-2011(02)00205-7

M3 - Journal article

C2 - 12623309

AN - SCOPUS:0037314520

SN - 0022-2011

VL - 82

SP - 97

EP - 102

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

IS - 2

ER -

Effects of long-term storage at -14°C on the survival of Neozygites fresenii (Entomophthorales: Neozygitaceae) in cotton aphids (Homoptera: Aphididae)

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this