Effects of long-term immobilization and recovery on human triceps surae and collagen turnover in the Achilles tendon in patients with healing ankle fracture

Britt Christensen; Eva Dyrberg; Per Aagaard; Susanne Enehjelm; Michael Krogsgaard; Michael Kjaer; Henning Langberg

doi:10.1152/japplphysiol.00201.2008

Effects of long-term immobilization and recovery on human triceps surae and collagen turnover in the Achilles tendon in patients with healing ankle fracture

Britt Christensen, Eva Dyrberg, Per Aagaard, Susanne Enehjelm, Michael Krogsgaard, Michael Kjaer, Henning Langberg

27 Citations (Scopus)

Abstract

The aim of the present study was to analyze how human tendon connective tissue responds to an approximately 7-wk period of immobilization and a remobilization period of a similar length, in patients with unilateral ankle fracture, which is currently unknown. Calf muscle cross-sectional area (CSA) decreased by 15% (5,316 to 4,517 mm2) and strength by 54% (239 to 110 N.m) in the immobilized leg after 7 wk. During the 7-wk remobilization, the CSA increased by 9% (to 4,943 mm2) and strength by 37% (to 176 Nm). Achilles tendon CSA did not change significantly during either immobilization or remobilization. Local collagen turnover was measured as the peritendinous concentrations of NH2-terminal propeptide of type I collagen (PINP) and COOH-terminal telopeptide region of type I collagen (ICTP), markers thought to be indexes of type I collagen synthesis and degradation, respectively. Both markers were increased (PINP: 257 vs. 56 ng/ml; ICTP: 9.8 vs. 2.1 microg/l) in the immobilized leg compared with the control leg after the 7 wk of immobilization, and levels decreased again in the immobilized leg during the recovery period (PINP: 103 vs. 44 ng/ml; ICTP: 4.2 vs. 1.9 microg/l). A significant reduction in calf muscle CSA and strength was found in relation to 7 wk of immobilization. Immobilization increased both collagen synthesis and degradation in tendon near tissue. However, it cannot be excluded that the facture of the ankle in close proximity could have affected these data. Remobilization increased muscle size and strength and tendon synthesis and degradation decreased to baseline levels. These dynamic changes in tendon connective tissue turnover were not associated with macroscopic changes in tendon size.

Original language	English
Journal	Journal of Applied Physiology
Volume	105
Issue number	2
Pages (from-to)	420-426
Number of pages	7
ISSN	8750-7587
DOIs	https://doi.org/10.1152/japplphysiol.00201.2008
Publication status	Published - 2008

Keywords

Achilles Tendon
Adult
Ankle Injuries
Biological Markers
Collagen Type I
Female
Fractures, Bone
Humans
Immobilization
Male
Microdialysis
Middle Aged
Muscle Fibers, Skeletal
Muscle Strength
Muscle, Skeletal
Peptide Fragments
Procollagen
Tomography, X-Ray Computed

Access to Document

10.1152/japplphysiol.00201.2008

Cite this

Effects of long-term immobilization and recovery on human triceps surae and collagen turnover in the Achilles tendon in patients with healing ankle fracture. / Christensen, Britt; Dyrberg, Eva; Aagaard, Per et al.

In: Journal of Applied Physiology, Vol. 105, No. 2, 2008, p. 420-426.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{0f797be0f84311ddb219000ea68e967b,

title = "Effects of long-term immobilization and recovery on human triceps surae and collagen turnover in the Achilles tendon in patients with healing ankle fracture",

abstract = "The aim of the present study was to analyze how human tendon connective tissue responds to an approximately 7-wk period of immobilization and a remobilization period of a similar length, in patients with unilateral ankle fracture, which is currently unknown. Calf muscle cross-sectional area (CSA) decreased by 15% (5,316 to 4,517 mm2) and strength by 54% (239 to 110 N.m) in the immobilized leg after 7 wk. During the 7-wk remobilization, the CSA increased by 9% (to 4,943 mm2) and strength by 37% (to 176 Nm). Achilles tendon CSA did not change significantly during either immobilization or remobilization. Local collagen turnover was measured as the peritendinous concentrations of NH2-terminal propeptide of type I collagen (PINP) and COOH-terminal telopeptide region of type I collagen (ICTP), markers thought to be indexes of type I collagen synthesis and degradation, respectively. Both markers were increased (PINP: 257 vs. 56 ng/ml; ICTP: 9.8 vs. 2.1 microg/l) in the immobilized leg compared with the control leg after the 7 wk of immobilization, and levels decreased again in the immobilized leg during the recovery period (PINP: 103 vs. 44 ng/ml; ICTP: 4.2 vs. 1.9 microg/l). A significant reduction in calf muscle CSA and strength was found in relation to 7 wk of immobilization. Immobilization increased both collagen synthesis and degradation in tendon near tissue. However, it cannot be excluded that the facture of the ankle in close proximity could have affected these data. Remobilization increased muscle size and strength and tendon synthesis and degradation decreased to baseline levels. These dynamic changes in tendon connective tissue turnover were not associated with macroscopic changes in tendon size.",

keywords = "Achilles Tendon, Adult, Ankle Injuries, Biological Markers, Collagen Type I, Female, Fractures, Bone, Humans, Immobilization, Male, Microdialysis, Middle Aged, Muscle Fibers, Skeletal, Muscle Strength, Muscle, Skeletal, Peptide Fragments, Procollagen, Tomography, X-Ray Computed",

author = "Britt Christensen and Eva Dyrberg and Per Aagaard and Susanne Enehjelm and Michael Krogsgaard and Michael Kjaer and Henning Langberg",

note = "IS - 8750-7587 (Print)LA - engPT - Journal ArticleRN - 0 (Biological Markers)RN - 0 (Collagen Type I)RN - 0 (Peptide Fragments)RN - 0 (Procollagen)RN - 0 (procollagen Type I N-terminal peptide)SB - IM",

year = "2008",

doi = "10.1152/japplphysiol.00201.2008",

language = "English",

volume = "105",

pages = "420--426",

journal = "Journal of Applied Physiology",

issn = "8750-7587",

publisher = "American Physiological Society",

number = "2",

}

TY - JOUR

T1 - Effects of long-term immobilization and recovery on human triceps surae and collagen turnover in the Achilles tendon in patients with healing ankle fracture

AU - Christensen, Britt

AU - Dyrberg, Eva

AU - Aagaard, Per

AU - Enehjelm, Susanne

AU - Krogsgaard, Michael

AU - Kjaer, Michael

AU - Langberg, Henning

N1 - IS - 8750-7587 (Print)LA - engPT - Journal ArticleRN - 0 (Biological Markers)RN - 0 (Collagen Type I)RN - 0 (Peptide Fragments)RN - 0 (Procollagen)RN - 0 (procollagen Type I N-terminal peptide)SB - IM

PY - 2008

Y1 - 2008

N2 - The aim of the present study was to analyze how human tendon connective tissue responds to an approximately 7-wk period of immobilization and a remobilization period of a similar length, in patients with unilateral ankle fracture, which is currently unknown. Calf muscle cross-sectional area (CSA) decreased by 15% (5,316 to 4,517 mm2) and strength by 54% (239 to 110 N.m) in the immobilized leg after 7 wk. During the 7-wk remobilization, the CSA increased by 9% (to 4,943 mm2) and strength by 37% (to 176 Nm). Achilles tendon CSA did not change significantly during either immobilization or remobilization. Local collagen turnover was measured as the peritendinous concentrations of NH2-terminal propeptide of type I collagen (PINP) and COOH-terminal telopeptide region of type I collagen (ICTP), markers thought to be indexes of type I collagen synthesis and degradation, respectively. Both markers were increased (PINP: 257 vs. 56 ng/ml; ICTP: 9.8 vs. 2.1 microg/l) in the immobilized leg compared with the control leg after the 7 wk of immobilization, and levels decreased again in the immobilized leg during the recovery period (PINP: 103 vs. 44 ng/ml; ICTP: 4.2 vs. 1.9 microg/l). A significant reduction in calf muscle CSA and strength was found in relation to 7 wk of immobilization. Immobilization increased both collagen synthesis and degradation in tendon near tissue. However, it cannot be excluded that the facture of the ankle in close proximity could have affected these data. Remobilization increased muscle size and strength and tendon synthesis and degradation decreased to baseline levels. These dynamic changes in tendon connective tissue turnover were not associated with macroscopic changes in tendon size.

AB - The aim of the present study was to analyze how human tendon connective tissue responds to an approximately 7-wk period of immobilization and a remobilization period of a similar length, in patients with unilateral ankle fracture, which is currently unknown. Calf muscle cross-sectional area (CSA) decreased by 15% (5,316 to 4,517 mm2) and strength by 54% (239 to 110 N.m) in the immobilized leg after 7 wk. During the 7-wk remobilization, the CSA increased by 9% (to 4,943 mm2) and strength by 37% (to 176 Nm). Achilles tendon CSA did not change significantly during either immobilization or remobilization. Local collagen turnover was measured as the peritendinous concentrations of NH2-terminal propeptide of type I collagen (PINP) and COOH-terminal telopeptide region of type I collagen (ICTP), markers thought to be indexes of type I collagen synthesis and degradation, respectively. Both markers were increased (PINP: 257 vs. 56 ng/ml; ICTP: 9.8 vs. 2.1 microg/l) in the immobilized leg compared with the control leg after the 7 wk of immobilization, and levels decreased again in the immobilized leg during the recovery period (PINP: 103 vs. 44 ng/ml; ICTP: 4.2 vs. 1.9 microg/l). A significant reduction in calf muscle CSA and strength was found in relation to 7 wk of immobilization. Immobilization increased both collagen synthesis and degradation in tendon near tissue. However, it cannot be excluded that the facture of the ankle in close proximity could have affected these data. Remobilization increased muscle size and strength and tendon synthesis and degradation decreased to baseline levels. These dynamic changes in tendon connective tissue turnover were not associated with macroscopic changes in tendon size.

KW - Achilles Tendon

KW - Adult

KW - Ankle Injuries

KW - Biological Markers

KW - Collagen Type I

KW - Female

KW - Fractures, Bone

KW - Humans

KW - Immobilization

KW - Male

KW - Microdialysis

KW - Middle Aged

KW - Muscle Fibers, Skeletal

KW - Muscle Strength

KW - Muscle, Skeletal

KW - Peptide Fragments

KW - Procollagen

KW - Tomography, X-Ray Computed

U2 - 10.1152/japplphysiol.00201.2008

DO - 10.1152/japplphysiol.00201.2008

M3 - Journal article

C2 - 18403455

SN - 8750-7587

VL - 105

SP - 420

EP - 426

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 2

ER -

Effects of long-term immobilization and recovery on human triceps surae and collagen turnover in the Achilles tendon in patients with healing ankle fracture

Abstract

Keywords

Access to Document

Fingerprint

Cite this