Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae)

Abir M. Mohamed Ibrahim; Karen A. Martinez-Swatson; Farid Benkaci-Ali; Federico Cozzi; Ferhat Zoulikha; Henrik T. Simonsen

doi:10.1016/j.jarmap.2017.09.002

Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae)

Abir M. Mohamed Ibrahim, Karen A. Martinez-Swatson, Farid Benkaci-Ali, Federico Cozzi, Ferhat Zoulikha, Henrik T. Simonsen^*

^*Corresponding author for this work

Section for Molecular Plant Biology

4 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Thapsia garganica L. roots are used in Algerian traditional medicine for a number of ailments. It is used in a poultice as an antitussive treatment of acute bronchitis and pneumonia, in preparations with milk or oil taken orally to treat common lung diseases, and with the direct application of root sections for the soothing of dental pains. Aim of the study: The objective of this study was to evaluate the combined effect of microwave assisted extraction and gamma irradiation on sesquiterpene lactones in T. garganica extracts Materials and methods: To evaluate the combined effect of microwave assisted extraction and gamma irradiation on the highly bioactive compounds found in extracts of Algerian T. garganica, samples from different locations in Algeria were prepared by extraction from dried leaf and root samples of dried plant material, using different extraction methods Quantification of the compounds of interest was done using an HPLC. The antioxidant activity extracts was determined using the two free radical scavenging assays: the 2,2-diphenyl-picryl-hydrazyl (DPPH) and the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS). Results: It was found that location and extraction method had significant impact on the phytochemical composition of extracts. Gamma irradiation was found to have no effect on the phytochemical composition of the plant extracts or on their antioxidant properties. Conclusion: The study has shown that microwave assisted extraction is an effective method for investigating chemical compounds in T. garganica and the results support the notion that gamma irradiation for sterilisation do not alter the chemical composition. The authors wish to clarify that we cannot recommend the usage of any parts of T. garganica, in any form, for any remedy due to its very high toxicity.

Original language	English
Journal	Journal of Applied Research on Medicinal and Aromatic Plants
Volume	8
Pages (from-to)	26-32
Number of pages	7
ISSN	2214-7861
DOIs	https://doi.org/10.1016/j.jarmap.2017.09.002
Publication status	Published - 2018

Keywords

Antioxidant
Gamma irradiation
Microwave assisted extraction
Thapsia garganica
Thapsigargin

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10.1016/j.jarmap.2017.09.002

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Mohamed Ibrahim, A. M., Martinez-Swatson, K. A., Benkaci-Ali, F., Cozzi, F., Zoulikha, F., & Simonsen, H. T. (2018). Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae). Journal of Applied Research on Medicinal and Aromatic Plants, 8, 26-32. https://doi.org/10.1016/j.jarmap.2017.09.002

Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae). / Mohamed Ibrahim, Abir M.; Martinez-Swatson, Karen A.; Benkaci-Ali, Farid et al.
In: Journal of Applied Research on Medicinal and Aromatic Plants, Vol. 8, 2018, p. 26-32.

Research output: Contribution to journal › Journal article › Research › peer-review

Mohamed Ibrahim, AM, Martinez-Swatson, KA, Benkaci-Ali, F, Cozzi, F, Zoulikha, F & Simonsen, HT 2018, 'Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae)', Journal of Applied Research on Medicinal and Aromatic Plants, vol. 8, pp. 26-32. https://doi.org/10.1016/j.jarmap.2017.09.002

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title = "Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae)",

abstract = "Ethnopharmacological relevance: Thapsia garganica L. roots are used in Algerian traditional medicine for a number of ailments. It is used in a poultice as an antitussive treatment of acute bronchitis and pneumonia, in preparations with milk or oil taken orally to treat common lung diseases, and with the direct application of root sections for the soothing of dental pains. Aim of the study: The objective of this study was to evaluate the combined effect of microwave assisted extraction and gamma irradiation on sesquiterpene lactones in T. garganica extracts Materials and methods: To evaluate the combined effect of microwave assisted extraction and gamma irradiation on the highly bioactive compounds found in extracts of Algerian T. garganica, samples from different locations in Algeria were prepared by extraction from dried leaf and root samples of dried plant material, using different extraction methods Quantification of the compounds of interest was done using an HPLC. The antioxidant activity extracts was determined using the two free radical scavenging assays: the 2,2-diphenyl-picryl-hydrazyl (DPPH) and the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS). Results: It was found that location and extraction method had significant impact on the phytochemical composition of extracts. Gamma irradiation was found to have no effect on the phytochemical composition of the plant extracts or on their antioxidant properties. Conclusion: The study has shown that microwave assisted extraction is an effective method for investigating chemical compounds in T. garganica and the results support the notion that gamma irradiation for sterilisation do not alter the chemical composition. The authors wish to clarify that we cannot recommend the usage of any parts of T. garganica, in any form, for any remedy due to its very high toxicity.",

keywords = "Antioxidant, Gamma irradiation, Microwave assisted extraction, Thapsia garganica, Thapsigargin",

author = "{Mohamed Ibrahim}, {Abir M.} and Martinez-Swatson, {Karen A.} and Farid Benkaci-Ali and Federico Cozzi and Ferhat Zoulikha and Simonsen, {Henrik T.}",

year = "2018",

doi = "10.1016/j.jarmap.2017.09.002",

language = "English",

volume = "8",

pages = "26--32",

journal = "Journal of Applied Research on Medicinal and Aromatic Plants",

issn = "2214-7861",

publisher = "Elsevier GmbH",

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TY - JOUR

T1 - Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae)

AU - Mohamed Ibrahim, Abir M.

AU - Martinez-Swatson, Karen A.

AU - Benkaci-Ali, Farid

AU - Cozzi, Federico

AU - Zoulikha, Ferhat

AU - Simonsen, Henrik T.

PY - 2018

Y1 - 2018

N2 - Ethnopharmacological relevance: Thapsia garganica L. roots are used in Algerian traditional medicine for a number of ailments. It is used in a poultice as an antitussive treatment of acute bronchitis and pneumonia, in preparations with milk or oil taken orally to treat common lung diseases, and with the direct application of root sections for the soothing of dental pains. Aim of the study: The objective of this study was to evaluate the combined effect of microwave assisted extraction and gamma irradiation on sesquiterpene lactones in T. garganica extracts Materials and methods: To evaluate the combined effect of microwave assisted extraction and gamma irradiation on the highly bioactive compounds found in extracts of Algerian T. garganica, samples from different locations in Algeria were prepared by extraction from dried leaf and root samples of dried plant material, using different extraction methods Quantification of the compounds of interest was done using an HPLC. The antioxidant activity extracts was determined using the two free radical scavenging assays: the 2,2-diphenyl-picryl-hydrazyl (DPPH) and the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS). Results: It was found that location and extraction method had significant impact on the phytochemical composition of extracts. Gamma irradiation was found to have no effect on the phytochemical composition of the plant extracts or on their antioxidant properties. Conclusion: The study has shown that microwave assisted extraction is an effective method for investigating chemical compounds in T. garganica and the results support the notion that gamma irradiation for sterilisation do not alter the chemical composition. The authors wish to clarify that we cannot recommend the usage of any parts of T. garganica, in any form, for any remedy due to its very high toxicity.

AB - Ethnopharmacological relevance: Thapsia garganica L. roots are used in Algerian traditional medicine for a number of ailments. It is used in a poultice as an antitussive treatment of acute bronchitis and pneumonia, in preparations with milk or oil taken orally to treat common lung diseases, and with the direct application of root sections for the soothing of dental pains. Aim of the study: The objective of this study was to evaluate the combined effect of microwave assisted extraction and gamma irradiation on sesquiterpene lactones in T. garganica extracts Materials and methods: To evaluate the combined effect of microwave assisted extraction and gamma irradiation on the highly bioactive compounds found in extracts of Algerian T. garganica, samples from different locations in Algeria were prepared by extraction from dried leaf and root samples of dried plant material, using different extraction methods Quantification of the compounds of interest was done using an HPLC. The antioxidant activity extracts was determined using the two free radical scavenging assays: the 2,2-diphenyl-picryl-hydrazyl (DPPH) and the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS). Results: It was found that location and extraction method had significant impact on the phytochemical composition of extracts. Gamma irradiation was found to have no effect on the phytochemical composition of the plant extracts or on their antioxidant properties. Conclusion: The study has shown that microwave assisted extraction is an effective method for investigating chemical compounds in T. garganica and the results support the notion that gamma irradiation for sterilisation do not alter the chemical composition. The authors wish to clarify that we cannot recommend the usage of any parts of T. garganica, in any form, for any remedy due to its very high toxicity.

KW - Antioxidant

KW - Gamma irradiation

KW - Microwave assisted extraction

KW - Thapsia garganica

KW - Thapsigargin

U2 - 10.1016/j.jarmap.2017.09.002

DO - 10.1016/j.jarmap.2017.09.002

M3 - Journal article

AN - SCOPUS:85030697972

SN - 2214-7861

VL - 8

SP - 26

EP - 32

JO - Journal of Applied Research on Medicinal and Aromatic Plants

JF - Journal of Applied Research on Medicinal and Aromatic Plants

ER -

Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae)

Abstract

Keywords

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