Effects of buffer composition and dilution on nanowire field-effect biosensors

Noemie Denise Carmen Lloret; Rune Schøneberg Frederiksen; Thor Christian Møller; Nathalie Ines Rieben; Shivendra Upadhyay; Luca De Vico; Jan Halborg Jensen; Jesper Nygård; Karen Laurence Martinez

doi:10.1088/0957-4484/24/3/035501

Effects of buffer composition and dilution on nanowire field-effect biosensors

Noemie Denise Carmen Lloret, Rune Schøneberg Frederiksen, Thor Christian Møller, Nathalie Ines Rieben, Shivendra Upadhyay, Luca De Vico, Jan Halborg Jensen, Jesper Nygård, Karen Laurence Martinez

33 Citations (Scopus)

Abstract

Nanowire-based field-effect transistors (FETs) can be used as ultra-sensitive and label-free biosensors for detecting protein-protein interactions. A way to increase the performance of such sensors is to dilute the sensing buffer drastically. However, we show here that this can have an important effect on the function of the proteins. Moreover, it is demonstrated that this dilution significantly affects the pH stability of the sensing buffer, which consequently impacts the charge of the protein and thus the response and signal-to-noise ratio in the sensing experiments. Three model systems are investigated experimentally to illustrate the impact on ligand-protein and protein-protein interactions. Simulations are performed to illustrate the effect on the performance of the sensors. Combining various parameters, the current study provides a means for evaluating and selecting the most appropriate buffer composition for bioFET measurements.

Original language	English
Article number	035501
Journal	Nanotechnology
Volume	24
Issue number	3
ISSN	0957-4484
DOIs	https://doi.org/10.1088/0957-4484/24/3/035501
Publication status	Published - 25 Jan 2013

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title = "Effects of buffer composition and dilution on nanowire field-effect biosensors",

abstract = "Nanowire-based field-effect transistors (FETs) can be used as ultra-sensitive and label-free biosensors for detecting protein-protein interactions. A way to increase the performance of such sensors is to dilute the sensing buffer drastically. However, we show here that this can have an important effect on the function of the proteins. Moreover, it is demonstrated that this dilution significantly affects the pH stability of the sensing buffer, which consequently impacts the charge of the protein and thus the response and signal-to-noise ratio in the sensing experiments. Three model systems are investigated experimentally to illustrate the impact on ligand-protein and protein-protein interactions. Simulations are performed to illustrate the effect on the performance of the sensors. Combining various parameters, the current study provides a means for evaluating and selecting the most appropriate buffer composition for bioFET measurements.",

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AU - Lloret, Noemie Denise Carmen

AU - Frederiksen, Rune Schøneberg

AU - Møller, Thor Christian

AU - Rieben, Nathalie Ines

AU - Upadhyay, Shivendra

AU - De Vico, Luca

AU - Jensen, Jan Halborg

AU - Nygård, Jesper

AU - Martinez, Karen Laurence

PY - 2013/1/25

Y1 - 2013/1/25

N2 - Nanowire-based field-effect transistors (FETs) can be used as ultra-sensitive and label-free biosensors for detecting protein-protein interactions. A way to increase the performance of such sensors is to dilute the sensing buffer drastically. However, we show here that this can have an important effect on the function of the proteins. Moreover, it is demonstrated that this dilution significantly affects the pH stability of the sensing buffer, which consequently impacts the charge of the protein and thus the response and signal-to-noise ratio in the sensing experiments. Three model systems are investigated experimentally to illustrate the impact on ligand-protein and protein-protein interactions. Simulations are performed to illustrate the effect on the performance of the sensors. Combining various parameters, the current study provides a means for evaluating and selecting the most appropriate buffer composition for bioFET measurements.

AB - Nanowire-based field-effect transistors (FETs) can be used as ultra-sensitive and label-free biosensors for detecting protein-protein interactions. A way to increase the performance of such sensors is to dilute the sensing buffer drastically. However, we show here that this can have an important effect on the function of the proteins. Moreover, it is demonstrated that this dilution significantly affects the pH stability of the sensing buffer, which consequently impacts the charge of the protein and thus the response and signal-to-noise ratio in the sensing experiments. Three model systems are investigated experimentally to illustrate the impact on ligand-protein and protein-protein interactions. Simulations are performed to illustrate the effect on the performance of the sensors. Combining various parameters, the current study provides a means for evaluating and selecting the most appropriate buffer composition for bioFET measurements.

U2 - 10.1088/0957-4484/24/3/035501

DO - 10.1088/0957-4484/24/3/035501

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JO - Nanotechnology

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Effects of buffer composition and dilution on nanowire field-effect biosensors

Abstract

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