Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial

P. Danielsen; B. Jorgensen; L.N. Jorgensen; M.S. Agren; Tonny Karlsmark

Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial

P. Danielsen, B. Jorgensen, L.N. Jorgensen, M.S. Agren, Tonny Karlsmark

51 Citations (Scopus)

Abstract

BACKGROUND: Autologous platelet-rich fibrin contains multiple growth factors. The aim of this randomized clinical trial was to study the effect of topical platelet-rich fibrin on epithelialization of donor sites and meshed split-thickness skin autografts. METHODS: Twenty consecutive leg ulcer patients (median age, 72.5 years) were enrolled between April 1, 2006, and January 31, 2007. Two adjacent donor-site wounds of similar size (57.3 cm versus 62.5 cm) and depth (286 mum versus 298 mum) were made by an air-driven dermatome on the same thigh. One donor wound and one-half of the autografted surgically revised leg ulcer were randomized by concealed allocation to platelet-rich fibrin, and the other donor wound and autografted half were not (control). Biopsy specimens (4 mm) from donor wounds were evaluated for percentage neoepidermal coverage in tissue sections immunostained for keratin on days 5 and 8. Epithelial barrier function, macroscopic healing, microbiology, and pain at dressing removal were assessed. Epithelialization of meshed autografts was assessed macroscopically. RESULTS: Epithelial coverage of donor wounds did not differ significantly between platelet-rich fibrin and control on day 5 (43.5 percent versus 34.4 percent, p = 0.65) or day 8 (76.6 percent versus 94.8 percent, p = 0.17). Transepidermal water loss was 75.6 g/m/hr in donor wounds treated with platelet-rich fibrin and 71.9 g/m/hr on day 8 in those without (p = 0.26). No statistically significant differences in macroscopic epithelialization between platelet-rich fibrin and control were found for donor wounds or autografts. Neither bacterial flora nor pain differed significantly between platelet-rich fibrin and control donor wounds. CONCLUSION: Epithelialization of donor wounds or the interstices of autografts was not significantly influenced by platelet-rich fibrin treatment
Udgivelsesdato: 2008/11

Original language	English
Journal	Plastic and Reconstructive Surgery
Volume	122
Issue number	5
Pages (from-to)	1431-1440
Number of pages	9
ISSN	0032-1052
Publication status	Published - 2008

Cite this

Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial. / Danielsen, P.; Jorgensen, B.; Jorgensen, L.N. et al.

In: Plastic and Reconstructive Surgery, Vol. 122, No. 5, 2008, p. 1431-1440.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial",

abstract = "BACKGROUND: Autologous platelet-rich fibrin contains multiple growth factors. The aim of this randomized clinical trial was to study the effect of topical platelet-rich fibrin on epithelialization of donor sites and meshed split-thickness skin autografts. METHODS: Twenty consecutive leg ulcer patients (median age, 72.5 years) were enrolled between April 1, 2006, and January 31, 2007. Two adjacent donor-site wounds of similar size (57.3 cm versus 62.5 cm) and depth (286 mum versus 298 mum) were made by an air-driven dermatome on the same thigh. One donor wound and one-half of the autografted surgically revised leg ulcer were randomized by concealed allocation to platelet-rich fibrin, and the other donor wound and autografted half were not (control). Biopsy specimens (4 mm) from donor wounds were evaluated for percentage neoepidermal coverage in tissue sections immunostained for keratin on days 5 and 8. Epithelial barrier function, macroscopic healing, microbiology, and pain at dressing removal were assessed. Epithelialization of meshed autografts was assessed macroscopically. RESULTS: Epithelial coverage of donor wounds did not differ significantly between platelet-rich fibrin and control on day 5 (43.5 percent versus 34.4 percent, p = 0.65) or day 8 (76.6 percent versus 94.8 percent, p = 0.17). Transepidermal water loss was 75.6 g/m/hr in donor wounds treated with platelet-rich fibrin and 71.9 g/m/hr on day 8 in those without (p = 0.26). No statistically significant differences in macroscopic epithelialization between platelet-rich fibrin and control were found for donor wounds or autografts. Neither bacterial flora nor pain differed significantly between platelet-rich fibrin and control donor wounds. CONCLUSION: Epithelialization of donor wounds or the interstices of autografts was not significantly influenced by platelet-rich fibrin treatment Udgivelsesdato: 2008/11",

author = "P. Danielsen and B. Jorgensen and L.N. Jorgensen and M.S. Agren and Tonny Karlsmark",

year = "2008",

language = "English",

volume = "122",

pages = "1431--1440",

journal = "Plastic and Reconstructive Surgery",

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TY - JOUR

T1 - Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial

AU - Danielsen, P.

AU - Jorgensen, B.

AU - Jorgensen, L.N.

AU - Agren, M.S.

AU - Karlsmark, Tonny

PY - 2008

Y1 - 2008

N2 - BACKGROUND: Autologous platelet-rich fibrin contains multiple growth factors. The aim of this randomized clinical trial was to study the effect of topical platelet-rich fibrin on epithelialization of donor sites and meshed split-thickness skin autografts. METHODS: Twenty consecutive leg ulcer patients (median age, 72.5 years) were enrolled between April 1, 2006, and January 31, 2007. Two adjacent donor-site wounds of similar size (57.3 cm versus 62.5 cm) and depth (286 mum versus 298 mum) were made by an air-driven dermatome on the same thigh. One donor wound and one-half of the autografted surgically revised leg ulcer were randomized by concealed allocation to platelet-rich fibrin, and the other donor wound and autografted half were not (control). Biopsy specimens (4 mm) from donor wounds were evaluated for percentage neoepidermal coverage in tissue sections immunostained for keratin on days 5 and 8. Epithelial barrier function, macroscopic healing, microbiology, and pain at dressing removal were assessed. Epithelialization of meshed autografts was assessed macroscopically. RESULTS: Epithelial coverage of donor wounds did not differ significantly between platelet-rich fibrin and control on day 5 (43.5 percent versus 34.4 percent, p = 0.65) or day 8 (76.6 percent versus 94.8 percent, p = 0.17). Transepidermal water loss was 75.6 g/m/hr in donor wounds treated with platelet-rich fibrin and 71.9 g/m/hr on day 8 in those without (p = 0.26). No statistically significant differences in macroscopic epithelialization between platelet-rich fibrin and control were found for donor wounds or autografts. Neither bacterial flora nor pain differed significantly between platelet-rich fibrin and control donor wounds. CONCLUSION: Epithelialization of donor wounds or the interstices of autografts was not significantly influenced by platelet-rich fibrin treatment Udgivelsesdato: 2008/11

AB - BACKGROUND: Autologous platelet-rich fibrin contains multiple growth factors. The aim of this randomized clinical trial was to study the effect of topical platelet-rich fibrin on epithelialization of donor sites and meshed split-thickness skin autografts. METHODS: Twenty consecutive leg ulcer patients (median age, 72.5 years) were enrolled between April 1, 2006, and January 31, 2007. Two adjacent donor-site wounds of similar size (57.3 cm versus 62.5 cm) and depth (286 mum versus 298 mum) were made by an air-driven dermatome on the same thigh. One donor wound and one-half of the autografted surgically revised leg ulcer were randomized by concealed allocation to platelet-rich fibrin, and the other donor wound and autografted half were not (control). Biopsy specimens (4 mm) from donor wounds were evaluated for percentage neoepidermal coverage in tissue sections immunostained for keratin on days 5 and 8. Epithelial barrier function, macroscopic healing, microbiology, and pain at dressing removal were assessed. Epithelialization of meshed autografts was assessed macroscopically. RESULTS: Epithelial coverage of donor wounds did not differ significantly between platelet-rich fibrin and control on day 5 (43.5 percent versus 34.4 percent, p = 0.65) or day 8 (76.6 percent versus 94.8 percent, p = 0.17). Transepidermal water loss was 75.6 g/m/hr in donor wounds treated with platelet-rich fibrin and 71.9 g/m/hr on day 8 in those without (p = 0.26). No statistically significant differences in macroscopic epithelialization between platelet-rich fibrin and control were found for donor wounds or autografts. Neither bacterial flora nor pain differed significantly between platelet-rich fibrin and control donor wounds. CONCLUSION: Epithelialization of donor wounds or the interstices of autografts was not significantly influenced by platelet-rich fibrin treatment Udgivelsesdato: 2008/11

M3 - Journal article

SN - 0032-1052

VL - 122

SP - 1431

EP - 1440

JO - Plastic and Reconstructive Surgery

JF - Plastic and Reconstructive Surgery

IS - 5

ER -