Effect of red light on development and quality of mammalian embryos

Rong Li; Kamilla Sofie Pedersen; Ying Liu; Hanne Skovsgaard Pedersen; Mette Lægdsmand; Lars F Rickelt; Michael Kühl; Henrik Callesen

doi:10.1007/s10815-014-0247-7

Effect of red light on development and quality of mammalian embryos

Rong Li, Kamilla Sofie Pedersen, Ying Liu, Hanne Skovsgaard Pedersen, Mette Lægdsmand, Lars F Rickelt, Michael Kühl, Henrik Callesen

Marine Biology

18 Citations (Scopus)

Abstract

Purpose: To assess irradiance and total energy dose from different microscopes during the in-vitro embryonic developmental cycle in mouse and pig and to evaluate its effect on embryonic development and quality in pig. Method: Spectral scalar irradiance (380-1050 nm) was measured by a fiber-optic microsensor in the focal plane of a dissection microscope, an inverted microscope and a time-lapse incubation system. Furthermore, the effect of three different red light levels was tested in the time-lapse system on mouse zygotes for 5 days, and on porcine zona-intact and zona-free parthenogenetically activated (PA) embryos for 6 days. Results: The time-lapse system used red light centered at 625 nm and with a lower irradiance level as compared to the white light irradiance levels on the dissection and inverted microscopes, which included more energetic radiation <550 nm. Even after 1000 times higher total energy dose of red light exposure in the time-lapse system, no significant difference was found neither in blastocyst development of mouse zygotes nor in blastocyst rates and total cell number of blastocysts of porcine PA embryos. Conclusions: Our results indicate that red light (625 nm, 0.34 W/m²) used in the time-lapse incubation system does not decrease the development and quality of blastocysts in both mouse zygotes and porcine PA embryos (both zona-intact and zona-free).

Original language	English
Journal	Journal of Assisted Reproduction and Genetics
Volume	31
Issue number	7
Pages (from-to)	795-801
Number of pages	7
ISSN	1058-0468
DOIs	https://doi.org/10.1007/s10815-014-0247-7
Publication status	Published - Jul 2014

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10.1007/s10815-014-0247-7

http://link.springer.com/article/10.1007/s10815-014-0247-7

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title = "Effect of red light on development and quality of mammalian embryos",

abstract = "Purpose: To assess irradiance and total energy dose from different microscopes during the in-vitro embryonic developmental cycle in mouse and pig and to evaluate its effect on embryonic development and quality in pig. Method: Spectral scalar irradiance (380-1050 nm) was measured by a fiber-optic microsensor in the focal plane of a dissection microscope, an inverted microscope and a time-lapse incubation system. Furthermore, the effect of three different red light levels was tested in the time-lapse system on mouse zygotes for 5 days, and on porcine zona-intact and zona-free parthenogenetically activated (PA) embryos for 6 days. Results: The time-lapse system used red light centered at 625 nm and with a lower irradiance level as compared to the white light irradiance levels on the dissection and inverted microscopes, which included more energetic radiation <550 nm. Even after 1000 times higher total energy dose of red light exposure in the time-lapse system, no significant difference was found neither in blastocyst development of mouse zygotes nor in blastocyst rates and total cell number of blastocysts of porcine PA embryos. Conclusions: Our results indicate that red light (625 nm, 0.34 W/m2) used in the time-lapse incubation system does not decrease the development and quality of blastocysts in both mouse zygotes and porcine PA embryos (both zona-intact and zona-free).",

author = "Rong Li and Pedersen, {Kamilla Sofie} and Ying Liu and Pedersen, {Hanne Skovsgaard} and Mette L{\ae}gdsmand and Rickelt, {Lars F} and Michael K{\"u}hl and Henrik Callesen",

year = "2014",

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doi = "10.1007/s10815-014-0247-7",

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TY - JOUR

T1 - Effect of red light on development and quality of mammalian embryos

AU - Li, Rong

AU - Pedersen, Kamilla Sofie

AU - Liu, Ying

AU - Pedersen, Hanne Skovsgaard

AU - Lægdsmand, Mette

AU - Rickelt, Lars F

AU - Kühl, Michael

AU - Callesen, Henrik

PY - 2014/7

Y1 - 2014/7

N2 - Purpose: To assess irradiance and total energy dose from different microscopes during the in-vitro embryonic developmental cycle in mouse and pig and to evaluate its effect on embryonic development and quality in pig. Method: Spectral scalar irradiance (380-1050 nm) was measured by a fiber-optic microsensor in the focal plane of a dissection microscope, an inverted microscope and a time-lapse incubation system. Furthermore, the effect of three different red light levels was tested in the time-lapse system on mouse zygotes for 5 days, and on porcine zona-intact and zona-free parthenogenetically activated (PA) embryos for 6 days. Results: The time-lapse system used red light centered at 625 nm and with a lower irradiance level as compared to the white light irradiance levels on the dissection and inverted microscopes, which included more energetic radiation <550 nm. Even after 1000 times higher total energy dose of red light exposure in the time-lapse system, no significant difference was found neither in blastocyst development of mouse zygotes nor in blastocyst rates and total cell number of blastocysts of porcine PA embryos. Conclusions: Our results indicate that red light (625 nm, 0.34 W/m2) used in the time-lapse incubation system does not decrease the development and quality of blastocysts in both mouse zygotes and porcine PA embryos (both zona-intact and zona-free).

AB - Purpose: To assess irradiance and total energy dose from different microscopes during the in-vitro embryonic developmental cycle in mouse and pig and to evaluate its effect on embryonic development and quality in pig. Method: Spectral scalar irradiance (380-1050 nm) was measured by a fiber-optic microsensor in the focal plane of a dissection microscope, an inverted microscope and a time-lapse incubation system. Furthermore, the effect of three different red light levels was tested in the time-lapse system on mouse zygotes for 5 days, and on porcine zona-intact and zona-free parthenogenetically activated (PA) embryos for 6 days. Results: The time-lapse system used red light centered at 625 nm and with a lower irradiance level as compared to the white light irradiance levels on the dissection and inverted microscopes, which included more energetic radiation <550 nm. Even after 1000 times higher total energy dose of red light exposure in the time-lapse system, no significant difference was found neither in blastocyst development of mouse zygotes nor in blastocyst rates and total cell number of blastocysts of porcine PA embryos. Conclusions: Our results indicate that red light (625 nm, 0.34 W/m2) used in the time-lapse incubation system does not decrease the development and quality of blastocysts in both mouse zygotes and porcine PA embryos (both zona-intact and zona-free).

U2 - 10.1007/s10815-014-0247-7

DO - 10.1007/s10815-014-0247-7

M3 - Journal article

C2 - 24854483

SN - 1058-0468

VL - 31

SP - 795

EP - 801

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

IS - 7

ER -

Effect of red light on development and quality of mammalian embryos

Abstract

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