Effect of protein intake and physical exercise on plasma amino acid pattern: 24hr studies on healthy adult males at energy balance

L Hambræus; A Forslund; A Andersson; H Van Beurden; Antoine E El-Khoury; G Hjorth; Roger M Olsson; Anders Mikael Sjödin; Vernon R Young

Effect of protein intake and physical exercise on plasma amino acid pattern: 24hr studies on healthy adult males at energy balance

L Hambræus, A Forslund, A Andersson, H Van Beurden, Antoine E El-Khoury, G Hjorth, Roger M Olsson, Anders Mikael Sjödin, Vernon R Young

Abstract

The effect of physical exercise on a "normal" and "high" protein intake(1 vs 2.5g/kg bw/24hrs) on plasma amino acid levels (PAAL) were followed during 24hr at energy balance in 8 and 6 healthy males, resp., after 6 days on a standardized diet and exercise program. The subjects were fasting until 12noon and then fed 10 identical meals hourly until 9PM. Physical exercise was performed at 45% of VO₂-max during fasting (8.30-10AM) as well as in a fed state (4-5.30PM). Energy turnover was determined by direct and indirect calorimetry and protein turnover evaluated by continuous infusion of ¹³C-leucine and ¹⁵N¹⁵N-urea. PAAL were determined every hr using an amino acid analyzer. Although the PAAL were increased during meals, a high protein intake resulted in lower PAAL values for most amino acids (AA), with exception of methionine, tyrosine and the branched-chain amino acids (BCAA). Physical activity slightly decreased BCAA levels at fasting but increased BCAA levels in fed state. Thus, a high protein intake at energy balance may not necessarily lead to increased availability of AA in peripheral tissues but PAAL appear to reflect the extent to which specific amino acids are involved in muscle and/or liver metabolism.

Original language	English
Journal	FASEB Journal
Volume	11
Issue number	3
Pages (from-to)	A150
Number of pages	1
ISSN	0892-6638
Publication status	Published - 1997
Externally published	Yes

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title = "Effect of protein intake and physical exercise on plasma amino acid pattern: 24hr studies on healthy adult males at energy balance",

abstract = "The effect of physical exercise on a {"}normal{"} and {"}high{"} protein intake(1 vs 2.5g/kg bw/24hrs) on plasma amino acid levels (PAAL) were followed during 24hr at energy balance in 8 and 6 healthy males, resp., after 6 days on a standardized diet and exercise program. The subjects were fasting until 12noon and then fed 10 identical meals hourly until 9PM. Physical exercise was performed at 45% of VO2-max during fasting (8.30-10AM) as well as in a fed state (4-5.30PM). Energy turnover was determined by direct and indirect calorimetry and protein turnover evaluated by continuous infusion of 13C-leucine and 15N15N-urea. PAAL were determined every hr using an amino acid analyzer. Although the PAAL were increased during meals, a high protein intake resulted in lower PAAL values for most amino acids (AA), with exception of methionine, tyrosine and the branched-chain amino acids (BCAA). Physical activity slightly decreased BCAA levels at fasting but increased BCAA levels in fed state. Thus, a high protein intake at energy balance may not necessarily lead to increased availability of AA in peripheral tissues but PAAL appear to reflect the extent to which specific amino acids are involved in muscle and/or liver metabolism.",

author = "L Hambr{\ae}us and A Forslund and A Andersson and {Van Beurden}, H and El-Khoury, {Antoine E} and G Hjorth and Olsson, {Roger M} and Sj{\"o}din, {Anders Mikael} and Young, {Vernon R}",

year = "1997",

language = "English",

volume = "11",

pages = "A150",

journal = "F A S E B Journal",

issn = "0892-6638",

publisher = "Federation of American Societies for Experimental Biology",

number = "3",

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TY - ABST

T1 - Effect of protein intake and physical exercise on plasma amino acid pattern

T2 - 24hr studies on healthy adult males at energy balance

AU - Hambræus, L

AU - Forslund, A

AU - Andersson, A

AU - Van Beurden, H

AU - El-Khoury, Antoine E

AU - Hjorth, G

AU - Olsson, Roger M

AU - Sjödin, Anders Mikael

AU - Young, Vernon R

PY - 1997

Y1 - 1997

N2 - The effect of physical exercise on a "normal" and "high" protein intake(1 vs 2.5g/kg bw/24hrs) on plasma amino acid levels (PAAL) were followed during 24hr at energy balance in 8 and 6 healthy males, resp., after 6 days on a standardized diet and exercise program. The subjects were fasting until 12noon and then fed 10 identical meals hourly until 9PM. Physical exercise was performed at 45% of VO2-max during fasting (8.30-10AM) as well as in a fed state (4-5.30PM). Energy turnover was determined by direct and indirect calorimetry and protein turnover evaluated by continuous infusion of 13C-leucine and 15N15N-urea. PAAL were determined every hr using an amino acid analyzer. Although the PAAL were increased during meals, a high protein intake resulted in lower PAAL values for most amino acids (AA), with exception of methionine, tyrosine and the branched-chain amino acids (BCAA). Physical activity slightly decreased BCAA levels at fasting but increased BCAA levels in fed state. Thus, a high protein intake at energy balance may not necessarily lead to increased availability of AA in peripheral tissues but PAAL appear to reflect the extent to which specific amino acids are involved in muscle and/or liver metabolism.

AB - The effect of physical exercise on a "normal" and "high" protein intake(1 vs 2.5g/kg bw/24hrs) on plasma amino acid levels (PAAL) were followed during 24hr at energy balance in 8 and 6 healthy males, resp., after 6 days on a standardized diet and exercise program. The subjects were fasting until 12noon and then fed 10 identical meals hourly until 9PM. Physical exercise was performed at 45% of VO2-max during fasting (8.30-10AM) as well as in a fed state (4-5.30PM). Energy turnover was determined by direct and indirect calorimetry and protein turnover evaluated by continuous infusion of 13C-leucine and 15N15N-urea. PAAL were determined every hr using an amino acid analyzer. Although the PAAL were increased during meals, a high protein intake resulted in lower PAAL values for most amino acids (AA), with exception of methionine, tyrosine and the branched-chain amino acids (BCAA). Physical activity slightly decreased BCAA levels at fasting but increased BCAA levels in fed state. Thus, a high protein intake at energy balance may not necessarily lead to increased availability of AA in peripheral tissues but PAAL appear to reflect the extent to which specific amino acids are involved in muscle and/or liver metabolism.

UR - http://www.scopus.com/inward/record.url?scp=33750135650&partnerID=8YFLogxK

M3 - Conference abstract in journal

AN - SCOPUS:33750135650

SN - 0892-6638

VL - 11

SP - A150

JO - F A S E B Journal

JF - F A S E B Journal

IS - 3

ER -

Effect of protein intake and physical exercise on plasma amino acid pattern: 24hr studies on healthy adult males at energy balance

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