Effect of protein intake and physical activity on 24-h pattern and rate of macronutrient utilization

Anders Forslund; Antoine E El-Khoury; Roger M Olsson; Anders Mikael Sjödin; Leif Hambraeus; Vernon R Young

doi:10.1152/ajpendo.1999.276.5.E964

Effect of protein intake and physical activity on 24-h pattern and rate of macronutrient utilization

Anders Forslund, Antoine E El-Khoury, Roger M Olsson, Anders Mikael Sjödin, Leif Hambraeus, Vernon R Young

31 Citations (Scopus)

Abstract

Effects of moderate physical activity (90 min at 45-50% of maximal O₂ uptake 2 times daily) and 'high, (2.5 g protein · kg^-1 · day^-1, n = 6) or 'normal' protein intake (1.0 g protein · kg^-1 · day^-1, n = 8) on the pattern and rate of 24-h macronutrient utilization in healthy adult men were compared after a diet-exercise-adjustment period of 6 days. Energy turnover (ET) was determined by indirect and direct (suit) calorimetry, and 'protein oxidation' was determined by a 24-h continuous intravenous infusion of [1- ¹³C]leucine. Subjects were in slight positive energy balance during both studies. Protein contributed to a higher (22 vs. 10%) and carbohydrate (CHO) a lower (33 vs. 58%) proportion of total 24-h ET on the high- vs. normal- protein intake. The highest contribution of fat to ET was seen postexercise during fasting (73 and 61% of ET for high and normal, respectively). With the high-protein diet the subjects were in a positive protein (P < 0.001) and CHO balance (P < 0.05) and a negative fat balance (P < 0.05). The increased ET postexercise was not explained by increased rates of urea production and/or protein synthesis.

Original language	English
Journal	American Journal of Physiology - Endocrinology and Metabolism
Volume	276
Issue number	5
Pages (from-to)	E964-E976
Number of pages	13
ISSN	0193-1849
DOIs	https://doi.org/10.1152/ajpendo.1999.276.5.E964
Publication status	Published - 1999
Externally published	Yes

Keywords

Carbohydrate
Direct calorimetry
Energy turnover
Fat
Indirect calorimetry
Macronutrient utilization
Obesity
Physical exercise
Protein
Stable isotopes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1152/ajpendo.1999.276.5.E964

Cite this

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title = "Effect of protein intake and physical activity on 24-h pattern and rate of macronutrient utilization",

abstract = "Effects of moderate physical activity (90 min at 45-50% of maximal O2 uptake 2 times daily) and 'high, (2.5 g protein · kg-1 · day-1, n = 6) or 'normal' protein intake (1.0 g protein · kg-1 · day-1, n = 8) on the pattern and rate of 24-h macronutrient utilization in healthy adult men were compared after a diet-exercise-adjustment period of 6 days. Energy turnover (ET) was determined by indirect and direct (suit) calorimetry, and 'protein oxidation' was determined by a 24-h continuous intravenous infusion of [1- 13C]leucine. Subjects were in slight positive energy balance during both studies. Protein contributed to a higher (22 vs. 10%) and carbohydrate (CHO) a lower (33 vs. 58%) proportion of total 24-h ET on the high- vs. normal- protein intake. The highest contribution of fat to ET was seen postexercise during fasting (73 and 61% of ET for high and normal, respectively). With the high-protein diet the subjects were in a positive protein (P < 0.001) and CHO balance (P < 0.05) and a negative fat balance (P < 0.05). The increased ET postexercise was not explained by increased rates of urea production and/or protein synthesis.",

keywords = "Carbohydrate, Direct calorimetry, Energy turnover, Fat, Indirect calorimetry, Macronutrient utilization, Obesity, Physical exercise, Protein, Stable isotopes",

author = "Anders Forslund and El-Khoury, {Antoine E} and Olsson, {Roger M} and Sj{\"o}din, {Anders Mikael} and Leif Hambraeus and Young, {Vernon R}",

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T1 - Effect of protein intake and physical activity on 24-h pattern and rate of macronutrient utilization

AU - Forslund, Anders

AU - El-Khoury, Antoine E

AU - Olsson, Roger M

AU - Sjödin, Anders Mikael

AU - Hambraeus, Leif

AU - Young, Vernon R

PY - 1999

Y1 - 1999

N2 - Effects of moderate physical activity (90 min at 45-50% of maximal O2 uptake 2 times daily) and 'high, (2.5 g protein · kg-1 · day-1, n = 6) or 'normal' protein intake (1.0 g protein · kg-1 · day-1, n = 8) on the pattern and rate of 24-h macronutrient utilization in healthy adult men were compared after a diet-exercise-adjustment period of 6 days. Energy turnover (ET) was determined by indirect and direct (suit) calorimetry, and 'protein oxidation' was determined by a 24-h continuous intravenous infusion of [1- 13C]leucine. Subjects were in slight positive energy balance during both studies. Protein contributed to a higher (22 vs. 10%) and carbohydrate (CHO) a lower (33 vs. 58%) proportion of total 24-h ET on the high- vs. normal- protein intake. The highest contribution of fat to ET was seen postexercise during fasting (73 and 61% of ET for high and normal, respectively). With the high-protein diet the subjects were in a positive protein (P < 0.001) and CHO balance (P < 0.05) and a negative fat balance (P < 0.05). The increased ET postexercise was not explained by increased rates of urea production and/or protein synthesis.

AB - Effects of moderate physical activity (90 min at 45-50% of maximal O2 uptake 2 times daily) and 'high, (2.5 g protein · kg-1 · day-1, n = 6) or 'normal' protein intake (1.0 g protein · kg-1 · day-1, n = 8) on the pattern and rate of 24-h macronutrient utilization in healthy adult men were compared after a diet-exercise-adjustment period of 6 days. Energy turnover (ET) was determined by indirect and direct (suit) calorimetry, and 'protein oxidation' was determined by a 24-h continuous intravenous infusion of [1- 13C]leucine. Subjects were in slight positive energy balance during both studies. Protein contributed to a higher (22 vs. 10%) and carbohydrate (CHO) a lower (33 vs. 58%) proportion of total 24-h ET on the high- vs. normal- protein intake. The highest contribution of fat to ET was seen postexercise during fasting (73 and 61% of ET for high and normal, respectively). With the high-protein diet the subjects were in a positive protein (P < 0.001) and CHO balance (P < 0.05) and a negative fat balance (P < 0.05). The increased ET postexercise was not explained by increased rates of urea production and/or protein synthesis.

KW - Carbohydrate

KW - Direct calorimetry

KW - Energy turnover

KW - Fat

KW - Indirect calorimetry

KW - Macronutrient utilization

KW - Obesity

KW - Physical exercise

KW - Protein

KW - Stable isotopes

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JF - A J P: Endocrinology and Metabolism (Online)

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ER -

Effect of protein intake and physical activity on 24-h pattern and rate of macronutrient utilization

Abstract

Keywords

UN SDGs

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