Effect of physical training on glucose transporter protein and mRNA levels in rat adipocytes

TY - JOUR

T1 - Effect of physical training on glucose transporter protein and mRNA levels in rat adipocytes

AU - Stallknecht, B

AU - Andersen, P H

AU - Vinten, J

AU - Bendtsen, L L

AU - Sibbersen, J

AU - Pedersen, O

AU - Galbo, H

N1 - Keywords: 3-O-Methylglucose; Adipose Tissue; Animals; Biological Transport; Glucose Transporter Type 1; Glucose Transporter Type 4; Male; Methylglucosides; Monosaccharide Transport Proteins; Muscle Proteins; Osmolar Concentration; Physical Conditioning, Animal; RNA, Messenger; Rats; Rats, Wistar

PY - 1993

Y1 - 1993

N2 - Physical training increases insulin-stimulated glucose transport and the number of glucose transporters in adipocytes measured by cytochalasin B binding. In the present study we used immunoblotting to measure the abundance of two glucose transporters (GLUT-4, GLUT-1) in white adipocytes from trained rats. Furthermore, the abundance of the mRNAs for these proteins and glucose transport was measured. Rats were swim-trained for 10 wk, and adipocytes were isolated from epididymal fat pads. The amount of GLUT-4/adipocyte volume unit was significantly higher in trained animals compared with both age- and cell size-matched animals. The amount of GLUT-4 mRNA was also increased by training and it decreased with increasing age. Furthermore, young age as well as training was accompanied by relatively low GLUT-4 protein/mRNA and relatively high overall GLUT-4 efficiency (recruitability and/or intrinsic activity). GLUT-1 protein and mRNA levels/adipocyte volume did not change with age or training.

AB - Physical training increases insulin-stimulated glucose transport and the number of glucose transporters in adipocytes measured by cytochalasin B binding. In the present study we used immunoblotting to measure the abundance of two glucose transporters (GLUT-4, GLUT-1) in white adipocytes from trained rats. Furthermore, the abundance of the mRNAs for these proteins and glucose transport was measured. Rats were swim-trained for 10 wk, and adipocytes were isolated from epididymal fat pads. The amount of GLUT-4/adipocyte volume unit was significantly higher in trained animals compared with both age- and cell size-matched animals. The amount of GLUT-4 mRNA was also increased by training and it decreased with increasing age. Furthermore, young age as well as training was accompanied by relatively low GLUT-4 protein/mRNA and relatively high overall GLUT-4 efficiency (recruitability and/or intrinsic activity). GLUT-1 protein and mRNA levels/adipocyte volume did not change with age or training.

M3 - Journal article

C2 - 8338145

SN - 0193-1849

VL - 265

SP - E128-E134

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

IS - 1 Pt 1

ER -