Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity

A Mancini; D Vitucci; G Labruna; E Imperlini; Morten Bredsgaard Randers; Jakob Friis Schmidt; H.  Hagman; Thomas Rostgaard Andersen; R Russo; S Orrù; Peter Krustrup; F Salvatore; P Buono

doi:10.1007/s00421-017-3562-8

Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity

A Mancini, D Vitucci, G Labruna, E Imperlini, Morten Bredsgaard Randers, Jakob Friis Schmidt, H. Hagman, Thomas Rostgaard Andersen, R Russo, S Orrù, Peter Krustrup, F Salvatore, P Buono

17 Citations (Scopus)

Abstract

PURPOSE: We investigated whether lifelong football training affects the expression of healthy longevity-related muscle molecular markers.

METHODS: Biopsies were collected from the vastus lateralis muscle of 10 lifelong football-trained men (68.2 ± 3.0 years) and of 10 active untrained healthy men (66.7 ± 1.3 years). Gene and protein expression was measured by RTqPCR on RNA and by western blotting on protein extracts from muscle biopsies, respectively.

RESULTS: The expression of AMPKα1/α2, NAMPT, TFAM and PGC1α, which are markers of oxidative metabolism, and MyHC β isoform expression was higher in the muscle of football-trained men vs untrained men. Also citrate synthase activity was higher in trained than in untrained men (109.3 ± 9.2 vs 75.1 ± 9.2 mU/mg). These findings were associated with a healthier body composition in trained than in untrained men [body weight: 78.2 ± 6.5 vs 91.2 ± 11.2 kg; body mass index BMI: 24.4 ± 1.6 vs 28.8 ± 4.0 kg m(-2); fat%: 22.6 ± 8.0 vs 31.4 ± 5.0%)] and with a higher maximal oxygen uptake (VO2max: 34.7 ± 3.8 vs 27.3 ± 4.0 ml/min/kg). Also the expression of proteins involved in DNA repair and in senescence suppression (Erk1/2, Akt and FoxM1) was higher in trained than in untrained men. At BMI- and age-adjusted multiple linear regression analysis, fat percentage was independently associated with Akt protein expression, and VO2max was independently associated with TFAM mRNA and with Erk1/2 protein expression.

CONCLUSIONS: Lifelong football training increases the expression of key markers involved in muscle oxidative metabolism, and in the DNA repair and senescence suppression pathways, thus providing the molecular basis for healthy longevity.

Original language	English
Journal	European Journal of Applied Physiology
Volume	117
Issue number	4
Pages (from-to)	721-730
Number of pages	10
ISSN	1439-6319
DOIs	https://doi.org/10.1007/s00421-017-3562-8
Publication status	Published - 1 Apr 2017

Keywords

Veteran football players
Long-term football training
Healthy aging
DNA repair and senescence suppression

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10.1007/s00421-017-3562-8

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Mancini, A., Vitucci, D., Labruna, G., Imperlini, E., Randers, M. B., Schmidt, J. F., Hagman, H., Andersen, T. R., Russo, R., Orrù, S., Krustrup, P., Salvatore, F., & Buono, P. (2017). Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity. European Journal of Applied Physiology, 117(4), 721-730. https://doi.org/10.1007/s00421-017-3562-8

Mancini, A, Vitucci, D, Labruna, G, Imperlini, E, Randers, MB, Schmidt, JF, Hagman, H, Andersen, TR, Russo, R, Orrù, S, Krustrup, P, Salvatore, F & Buono, P 2017, 'Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity', European Journal of Applied Physiology, vol. 117, no. 4, pp. 721-730. https://doi.org/10.1007/s00421-017-3562-8

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title = "Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity",

abstract = "PURPOSE: We investigated whether lifelong football training affects the expression of healthy longevity-related muscle molecular markers.METHODS: Biopsies were collected from the vastus lateralis muscle of 10 lifelong football-trained men (68.2 ± 3.0 years) and of 10 active untrained healthy men (66.7 ± 1.3 years). Gene and protein expression was measured by RTqPCR on RNA and by western blotting on protein extracts from muscle biopsies, respectively.RESULTS: The expression of AMPKα1/α2, NAMPT, TFAM and PGC1α, which are markers of oxidative metabolism, and MyHC β isoform expression was higher in the muscle of football-trained men vs untrained men. Also citrate synthase activity was higher in trained than in untrained men (109.3 ± 9.2 vs 75.1 ± 9.2 mU/mg). These findings were associated with a healthier body composition in trained than in untrained men [body weight: 78.2 ± 6.5 vs 91.2 ± 11.2 kg; body mass index BMI: 24.4 ± 1.6 vs 28.8 ± 4.0 kg m(-2); fat%: 22.6 ± 8.0 vs 31.4 ± 5.0%)] and with a higher maximal oxygen uptake (VO2max: 34.7 ± 3.8 vs 27.3 ± 4.0 ml/min/kg). Also the expression of proteins involved in DNA repair and in senescence suppression (Erk1/2, Akt and FoxM1) was higher in trained than in untrained men. At BMI- and age-adjusted multiple linear regression analysis, fat percentage was independently associated with Akt protein expression, and VO2max was independently associated with TFAM mRNA and with Erk1/2 protein expression.CONCLUSIONS: Lifelong football training increases the expression of key markers involved in muscle oxidative metabolism, and in the DNA repair and senescence suppression pathways, thus providing the molecular basis for healthy longevity.",

keywords = "Veteran football players, Long-term football training, Healthy aging, DNA repair and senescence suppression",

author = "A Mancini and D Vitucci and G Labruna and E Imperlini and Randers, {Morten Bredsgaard} and Schmidt, {Jakob Friis} and H. Hagman and Andersen, {Thomas Rostgaard} and R Russo and S Orr{\`u} and Peter Krustrup and F Salvatore and P Buono",

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doi = "10.1007/s00421-017-3562-8",

language = "English",

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pages = "721--730",

journal = "European Journal of Applied Physiology",

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TY - JOUR

T1 - Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity

AU - Mancini, A

AU - Vitucci, D

AU - Labruna, G

AU - Imperlini, E

AU - Randers, Morten Bredsgaard

AU - Schmidt, Jakob Friis

AU - Hagman, H.

AU - Andersen, Thomas Rostgaard

AU - Russo, R

AU - Orrù, S

AU - Krustrup, Peter

AU - Salvatore, F

AU - Buono, P

N1 - CURIS 2017 NEXS 076

PY - 2017/4/1

Y1 - 2017/4/1

N2 - PURPOSE: We investigated whether lifelong football training affects the expression of healthy longevity-related muscle molecular markers.METHODS: Biopsies were collected from the vastus lateralis muscle of 10 lifelong football-trained men (68.2 ± 3.0 years) and of 10 active untrained healthy men (66.7 ± 1.3 years). Gene and protein expression was measured by RTqPCR on RNA and by western blotting on protein extracts from muscle biopsies, respectively.RESULTS: The expression of AMPKα1/α2, NAMPT, TFAM and PGC1α, which are markers of oxidative metabolism, and MyHC β isoform expression was higher in the muscle of football-trained men vs untrained men. Also citrate synthase activity was higher in trained than in untrained men (109.3 ± 9.2 vs 75.1 ± 9.2 mU/mg). These findings were associated with a healthier body composition in trained than in untrained men [body weight: 78.2 ± 6.5 vs 91.2 ± 11.2 kg; body mass index BMI: 24.4 ± 1.6 vs 28.8 ± 4.0 kg m(-2); fat%: 22.6 ± 8.0 vs 31.4 ± 5.0%)] and with a higher maximal oxygen uptake (VO2max: 34.7 ± 3.8 vs 27.3 ± 4.0 ml/min/kg). Also the expression of proteins involved in DNA repair and in senescence suppression (Erk1/2, Akt and FoxM1) was higher in trained than in untrained men. At BMI- and age-adjusted multiple linear regression analysis, fat percentage was independently associated with Akt protein expression, and VO2max was independently associated with TFAM mRNA and with Erk1/2 protein expression.CONCLUSIONS: Lifelong football training increases the expression of key markers involved in muscle oxidative metabolism, and in the DNA repair and senescence suppression pathways, thus providing the molecular basis for healthy longevity.

AB - PURPOSE: We investigated whether lifelong football training affects the expression of healthy longevity-related muscle molecular markers.METHODS: Biopsies were collected from the vastus lateralis muscle of 10 lifelong football-trained men (68.2 ± 3.0 years) and of 10 active untrained healthy men (66.7 ± 1.3 years). Gene and protein expression was measured by RTqPCR on RNA and by western blotting on protein extracts from muscle biopsies, respectively.RESULTS: The expression of AMPKα1/α2, NAMPT, TFAM and PGC1α, which are markers of oxidative metabolism, and MyHC β isoform expression was higher in the muscle of football-trained men vs untrained men. Also citrate synthase activity was higher in trained than in untrained men (109.3 ± 9.2 vs 75.1 ± 9.2 mU/mg). These findings were associated with a healthier body composition in trained than in untrained men [body weight: 78.2 ± 6.5 vs 91.2 ± 11.2 kg; body mass index BMI: 24.4 ± 1.6 vs 28.8 ± 4.0 kg m(-2); fat%: 22.6 ± 8.0 vs 31.4 ± 5.0%)] and with a higher maximal oxygen uptake (VO2max: 34.7 ± 3.8 vs 27.3 ± 4.0 ml/min/kg). Also the expression of proteins involved in DNA repair and in senescence suppression (Erk1/2, Akt and FoxM1) was higher in trained than in untrained men. At BMI- and age-adjusted multiple linear regression analysis, fat percentage was independently associated with Akt protein expression, and VO2max was independently associated with TFAM mRNA and with Erk1/2 protein expression.CONCLUSIONS: Lifelong football training increases the expression of key markers involved in muscle oxidative metabolism, and in the DNA repair and senescence suppression pathways, thus providing the molecular basis for healthy longevity.

KW - Veteran football players

KW - Long-term football training

KW - Healthy aging

KW - DNA repair and senescence suppression

U2 - 10.1007/s00421-017-3562-8

DO - 10.1007/s00421-017-3562-8

M3 - Journal article

C2 - 28251397

SN - 1439-6319

VL - 117

SP - 721

EP - 730

JO - European Journal of Applied Physiology

JF - European Journal of Applied Physiology

IS - 4

ER -

Effect of lifelong football training on the expression of muscle molecular markers involved in healthy longevity

Abstract

Keywords

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