Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism

Nikolai Nordsborg; Jens Bangsbo; Henriette Pilegaard

doi:10.1152/japplphysiol.00257.2003

Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism

Nikolai Nordsborg, Jens Bangsbo, Henriette Pilegaard

Molecular Integrative Physiology

40 Citations (Scopus)

Abstract

Changes in gene expression during recovery from high-intensity, intermittent, one-legged exercise were studied before and after 5.5 wk of training. Genes related to metabolism, as well as Na+, K+, and pH homeostasis, were selected for analyses. After the same work was performed before and after the training period, several muscle biopsies were obtained from vastus lateralis muscle. In the untrained state, the Na+-K+-ATPase alpha1-subunit mRNA level was approximately threefold higher (P < 0.01) at 0, 1, and 3 h after exercise, relative to the preexercise resting level. After 3-5 h of recovery in the untrained state, pyruvate dehydrogenase kinase 4 and hexokinase II mRNA levels were elevated 13-fold (P < 0.001) and 6-fold (P < 0.01), respectively. However, after the training period, only pyruvate dehydrogenase kinase 4 mRNA levels were elevated (P < 0.05) during the recovery period. No changes in resting mRNA levels were observed as a result of training. In conclusion, cellular adaptations to high-intensity exercise training may, in part, be induced by transcriptional regulation. After training, the transcriptional response to an exercise bout at a given workload is diminished.

Original language	English
Journal	Journal of Applied Physiology
Volume	95
Issue number	3
Pages (from-to)	1201-1206
Number of pages	6
ISSN	8750-7587
DOIs	https://doi.org/10.1152/japplphysiol.00257.2003
Publication status	Published - 2003

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10.1152/japplphysiol.00257.2003

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@article{54f39010f0d911dcbee902004c4f4f50,

title = "Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism",

abstract = "Changes in gene expression during recovery from high-intensity, intermittent, one-legged exercise were studied before and after 5.5 wk of training. Genes related to metabolism, as well as Na+, K+, and pH homeostasis, were selected for analyses. After the same work was performed before and after the training period, several muscle biopsies were obtained from vastus lateralis muscle. In the untrained state, the Na+-K+-ATPase alpha1-subunit mRNA level was approximately threefold higher (P < 0.01) at 0, 1, and 3 h after exercise, relative to the preexercise resting level. After 3-5 h of recovery in the untrained state, pyruvate dehydrogenase kinase 4 and hexokinase II mRNA levels were elevated 13-fold (P < 0.001) and 6-fold (P < 0.01), respectively. However, after the training period, only pyruvate dehydrogenase kinase 4 mRNA levels were elevated (P < 0.05) during the recovery period. No changes in resting mRNA levels were observed as a result of training. In conclusion, cellular adaptations to high-intensity exercise training may, in part, be induced by transcriptional regulation. After training, the transcriptional response to an exercise bout at a given workload is diminished.",

author = "Nikolai Nordsborg and Jens Bangsbo and Henriette Pilegaard",

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doi = "10.1152/japplphysiol.00257.2003",

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TY - JOUR

T1 - Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism

AU - Nordsborg, Nikolai

AU - Bangsbo, Jens

AU - Pilegaard, Henriette

N1 - CURIS 2007 5200 184

PY - 2003

Y1 - 2003

N2 - Changes in gene expression during recovery from high-intensity, intermittent, one-legged exercise were studied before and after 5.5 wk of training. Genes related to metabolism, as well as Na+, K+, and pH homeostasis, were selected for analyses. After the same work was performed before and after the training period, several muscle biopsies were obtained from vastus lateralis muscle. In the untrained state, the Na+-K+-ATPase alpha1-subunit mRNA level was approximately threefold higher (P < 0.01) at 0, 1, and 3 h after exercise, relative to the preexercise resting level. After 3-5 h of recovery in the untrained state, pyruvate dehydrogenase kinase 4 and hexokinase II mRNA levels were elevated 13-fold (P < 0.001) and 6-fold (P < 0.01), respectively. However, after the training period, only pyruvate dehydrogenase kinase 4 mRNA levels were elevated (P < 0.05) during the recovery period. No changes in resting mRNA levels were observed as a result of training. In conclusion, cellular adaptations to high-intensity exercise training may, in part, be induced by transcriptional regulation. After training, the transcriptional response to an exercise bout at a given workload is diminished.

AB - Changes in gene expression during recovery from high-intensity, intermittent, one-legged exercise were studied before and after 5.5 wk of training. Genes related to metabolism, as well as Na+, K+, and pH homeostasis, were selected for analyses. After the same work was performed before and after the training period, several muscle biopsies were obtained from vastus lateralis muscle. In the untrained state, the Na+-K+-ATPase alpha1-subunit mRNA level was approximately threefold higher (P < 0.01) at 0, 1, and 3 h after exercise, relative to the preexercise resting level. After 3-5 h of recovery in the untrained state, pyruvate dehydrogenase kinase 4 and hexokinase II mRNA levels were elevated 13-fold (P < 0.001) and 6-fold (P < 0.01), respectively. However, after the training period, only pyruvate dehydrogenase kinase 4 mRNA levels were elevated (P < 0.05) during the recovery period. No changes in resting mRNA levels were observed as a result of training. In conclusion, cellular adaptations to high-intensity exercise training may, in part, be induced by transcriptional regulation. After training, the transcriptional response to an exercise bout at a given workload is diminished.

U2 - 10.1152/japplphysiol.00257.2003

DO - 10.1152/japplphysiol.00257.2003

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SN - 8750-7587

VL - 95

SP - 1201

EP - 1206

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 3

ER -