Abstract
Expression of the proto-oncogenes c-fos and c-jun was analysed in the insulin producing rat tumor cell line, RIN 5AH. Addition of fetal calf serum (FCS) to serum-starved cells in the presence of cycloheximid induced a modest increase in c-fos and c-jun mRNA levels, whereas growth hormone (GH) in the presence of cycloheximid had little or no effect, when added to RIN 5AH cells maintained in 0.5% FCS for 2 days prior to stimulation. Activation of protein kinase C by phorbol ester lead to increased c-jun and c-fos mRNA levels, whereas activation of adenylate cyclase by forskolin increased c-fos mRNA levels. These results suggest that the effects of GH on insulin producing cells are not mediated by activation of c-fos and c-jun transcription.
| Original language | English |
|---|---|
| Journal | Biomedica Biochimica Acta |
| Volume | 49 |
| Issue number | 12 |
| Pages (from-to) | 1171-5 |
| Number of pages | 5 |
| ISSN | 0232-766X |
| Publication status | Published - 1990 |
Keywords
- Adenylate Cyclase
- Animals
- Blood
- Cycloheximide
- DNA Probes
- DNA-Binding Proteins
- Enzyme Activation
- Forskolin
- Gene Expression Regulation
- Insulin
- Insulinoma
- Phorbol 12,13-Dibutyrate
- Protein Kinase C
- Protein-Tyrosine Kinases
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-fos
- Proto-Oncogene Proteins c-jun
- RNA, Messenger
- Rats
- Somatomedins
- Transcription Factors
- Tumor Cells, Cultured