Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins

Marcel Skejovic Joehnke; Susanne Sørensen; Charlotte Bjergegaard; Keld Ejdrup Markedal; Jens Christian Sørensen

doi:10.2478/pjfns-2018-0005

Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins

Marcel Skejovic Joehnke, Susanne Sørensen, Charlotte Bjergegaard, Keld Ejdrup Markedal, Jens Christian Sørensen

6 Citations (Scopus)

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Abstract

Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.

Original language	English
Journal	Polish Journal of Food and Nutrition Sciences
Volume	68
Issue number	4
Pages (from-to)	335-345
ISSN	1230-0322
DOIs	https://doi.org/10.2478/pjfns-2018-0005
Publication status	Published - 1 Dec 2018

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Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin ProteinsFinal published version, 440 KBLicence: CC BY-NC-ND

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title = "Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins",

abstract = "Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.",

author = "Joehnke, {Marcel Skejovic} and Susanne S{\o}rensen and Charlotte Bjergegaard and Markedal, {Keld Ejdrup} and S{\o}rensen, {Jens Christian}",

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T1 - Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins

AU - Joehnke, Marcel Skejovic

AU - Sørensen, Susanne

AU - Bjergegaard, Charlotte

AU - Markedal, Keld Ejdrup

AU - Sørensen, Jens Christian

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.

AB - Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.

U2 - 10.2478/pjfns-2018-0005

DO - 10.2478/pjfns-2018-0005

M3 - Journal article

SN - 1230-0322

VL - 68

SP - 335

EP - 345

JO - Polish Journal of Food and Nutrition Sciences

JF - Polish Journal of Food and Nutrition Sciences

IS - 4

ER -

Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins

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