Effect of cheese and butter intake on metabolites in urine using an untargeted metabolomics approach

TY - JOUR

T1 - Effect of cheese and butter intake on metabolites in urine using an untargeted metabolomics approach

AU - Hjerpsted, Julie Bousgaard

AU - Ritz, Christian

AU - Schou, Simon Stubbe

AU - Tholstrup, Tine

AU - Dragsted, Lars Ove

N1 - CURIS 2014 NEXS 249

PY - 2014/12

Y1 - 2014/12

N2 - Cheese intake has been shown to decrease total cholesterol and LDL cholesterol concentrations when compared to butter of equal fat content. An untargeted metabolite profiling may reveal exposure markers of cheese but may also contribute with markers which can help explain how the intake of cheese affects cholesterol concentrations. Twenty-three subjects collected 2 × 24 h urine samples after 6 weeks of cheese and 6 weeks of butter intake with equal amounts of fat in a cross-over intervention study. The samples were analyzed by UPLC-QTOF/MS. A two-step univariate data analysis approach using linear mixed model was applied separately for positive and negative ionization mode: In the first step a total of 44 features related to treatment were identified and in the second step 36 of these features were related to total cholesterol concentrations. Cheese intake resulted in increased urinary indoxyl sulfate, xanthurenic acid, tyramine sulfate, 4-hydroxyphenylacetic acid, isovalerylglutamic acid and several acylglycines including isovalerylglycine, tiglylglycine and isobutyrylglycine when compared to butter intake of equal fat content. The biological mechanisms of action linking the metabolites to cholesterol concentrations need to be further explored.

AB - Cheese intake has been shown to decrease total cholesterol and LDL cholesterol concentrations when compared to butter of equal fat content. An untargeted metabolite profiling may reveal exposure markers of cheese but may also contribute with markers which can help explain how the intake of cheese affects cholesterol concentrations. Twenty-three subjects collected 2 × 24 h urine samples after 6 weeks of cheese and 6 weeks of butter intake with equal amounts of fat in a cross-over intervention study. The samples were analyzed by UPLC-QTOF/MS. A two-step univariate data analysis approach using linear mixed model was applied separately for positive and negative ionization mode: In the first step a total of 44 features related to treatment were identified and in the second step 36 of these features were related to total cholesterol concentrations. Cheese intake resulted in increased urinary indoxyl sulfate, xanthurenic acid, tyramine sulfate, 4-hydroxyphenylacetic acid, isovalerylglutamic acid and several acylglycines including isovalerylglycine, tiglylglycine and isobutyrylglycine when compared to butter intake of equal fat content. The biological mechanisms of action linking the metabolites to cholesterol concentrations need to be further explored.

KW - Acylglycine

KW - Cholesterol

KW - Dairy

KW - Metabonomics

KW - Tyramine

KW - UPLC-MS/QTOF

U2 - 10.1007/s11306-014-0657-7

DO - 10.1007/s11306-014-0657-7

M3 - Journal article

SN - 1573-3882

VL - 10

SP - 1176

EP - 1185

JO - Metabolomics

JF - Metabolomics

IS - 6

ER -