Effect of administration of oral contraceptives in vivo on collagen synthesis in tendon and muscle connective tissue in young women

M Hansen; B F Miller; L Holm; S Doessing; S G Petersen; D Skovgaard; J Frystyk; A Flyvbjerg; S Koskinen; Jessica Pingel; M Kjaer; H Langberg

doi:10.1152/japplphysiol.90933.2008

Effect of administration of oral contraceptives in vivo on collagen synthesis in tendon and muscle connective tissue in young women

M Hansen, B F Miller, L Holm, S Doessing, S G Petersen, D Skovgaard, J Frystyk, A Flyvbjerg, S Koskinen, Jessica Pingel, M Kjaer, H Langberg

Damage and Repair

71 Citations (Scopus)

Abstract

Women are at greater risk than men for certain kinds of diseases and injuries, which may at least partly be caused by sex hormonal differences. We aimed to test the influence of estradiol in vivo on collagen synthesis in tendon, bone, and muscle. Two groups of young, healthy women similar in age, body composition, and exercise-training status were included. The two groups were either habitual users of oral contraceptives exposed to a high concentration of synthetic estradiol and progestogens (OC, n = 11), or non-OC-users tested in the follicular phase of the menstrual cycle characterized by low concentrations of estradiol and progesterone (control, n = 12). Subjects performed 1 h of one-legged kicking exercise. The next day collagen fractional synthesis rates (FSR) in tendon and muscle connective tissue were measured after a flooding dose of [(13)C]proline followed by biopsies from the patellar tendon and vastus lateralis in both legs. Simultaneously, microdialysis catheters were inserted in vastus lateralis and in front of the patellar tendon for measurement of insulin-like growth factor I (IGF-I) and its binding proteins. Serum NH(2)-terminal propeptide of type I collagen (PINP) and urine COOH-terminal telopeptides of type-I collagen (CTX-I) were measured as markers for bone synthesis and breakdown, respectively. Tendon FSR and PINP were lower in OC compared with control. An increase in muscle collagen FSR postexercise was only observed in control (P < 0.05). Furthermore, the results indicate a lower bioavailability of IGF-I in OC. In conclusion, synthetic female sex hormones administered as OC had an inhibiting effect on collagen synthesis in tendon, bone, and muscle connective tissue, which may be related to a lower bioavailability of IGF-I.

Original language	English
Journal	Journal of Applied Physiology
Volume	106
Issue number	4
Pages (from-to)	1435-43
Number of pages	9
ISSN	8750-7587
DOIs	https://doi.org/10.1152/japplphysiol.90933.2008
Publication status	Published - 2009

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10.1152/japplphysiol.90933.2008

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Hansen, M., Miller, B. F., Holm, L., Doessing, S., Petersen, S. G., Skovgaard, D., Frystyk, J., Flyvbjerg, A., Koskinen, S., Pingel, J., Kjaer, M., & Langberg, H. (2009). Effect of administration of oral contraceptives in vivo on collagen synthesis in tendon and muscle connective tissue in young women. Journal of Applied Physiology, 106(4), 1435-43. https://doi.org/10.1152/japplphysiol.90933.2008

Hansen, M, Miller, BF, Holm, L, Doessing, S, Petersen, SG, Skovgaard, D, Frystyk, J, Flyvbjerg, A, Koskinen, S, Pingel, J , Kjaer, M & Langberg, H 2009, 'Effect of administration of oral contraceptives in vivo on collagen synthesis in tendon and muscle connective tissue in young women', Journal of Applied Physiology, vol. 106, no. 4, pp. 1435-43. https://doi.org/10.1152/japplphysiol.90933.2008

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title = "Effect of administration of oral contraceptives in vivo on collagen synthesis in tendon and muscle connective tissue in young women",

abstract = "Women are at greater risk than men for certain kinds of diseases and injuries, which may at least partly be caused by sex hormonal differences. We aimed to test the influence of estradiol in vivo on collagen synthesis in tendon, bone, and muscle. Two groups of young, healthy women similar in age, body composition, and exercise-training status were included. The two groups were either habitual users of oral contraceptives exposed to a high concentration of synthetic estradiol and progestogens (OC, n = 11), or non-OC-users tested in the follicular phase of the menstrual cycle characterized by low concentrations of estradiol and progesterone (control, n = 12). Subjects performed 1 h of one-legged kicking exercise. The next day collagen fractional synthesis rates (FSR) in tendon and muscle connective tissue were measured after a flooding dose of [(13)C]proline followed by biopsies from the patellar tendon and vastus lateralis in both legs. Simultaneously, microdialysis catheters were inserted in vastus lateralis and in front of the patellar tendon for measurement of insulin-like growth factor I (IGF-I) and its binding proteins. Serum NH(2)-terminal propeptide of type I collagen (PINP) and urine COOH-terminal telopeptides of type-I collagen (CTX-I) were measured as markers for bone synthesis and breakdown, respectively. Tendon FSR and PINP were lower in OC compared with control. An increase in muscle collagen FSR postexercise was only observed in control (P < 0.05). Furthermore, the results indicate a lower bioavailability of IGF-I in OC. In conclusion, synthetic female sex hormones administered as OC had an inhibiting effect on collagen synthesis in tendon, bone, and muscle connective tissue, which may be related to a lower bioavailability of IGF-I.",

author = "M Hansen and Miller, {B F} and L Holm and S Doessing and Petersen, {S G} and D Skovgaard and J Frystyk and A Flyvbjerg and S Koskinen and Jessica Pingel and M Kjaer and H Langberg",

note = "Keywords: Adult; Bone and Bones; Collagen; Connective Tissue; Contraceptives, Oral, Hormonal; Energy Metabolism; Exercise; Exercise Test; Female; Growth Hormone; Humans; Insulin-Like Growth Factor Binding Proteins; Insulin-Like Growth Factor I; Kinetics; Microdialysis; Muscle, Skeletal; Proline; Tendons; Young Adult",

year = "2009",

doi = "10.1152/japplphysiol.90933.2008",

language = "English",

volume = "106",

pages = "1435--43",

journal = "Journal of Applied Physiology",

issn = "8750-7587",

publisher = "American Physiological Society",

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TY - JOUR

T1 - Effect of administration of oral contraceptives in vivo on collagen synthesis in tendon and muscle connective tissue in young women

AU - Hansen, M

AU - Miller, B F

AU - Holm, L

AU - Doessing, S

AU - Petersen, S G

AU - Skovgaard, D

AU - Frystyk, J

AU - Flyvbjerg, A

AU - Koskinen, S

AU - Pingel, Jessica

AU - Kjaer, M

AU - Langberg, H

N1 - Keywords: Adult; Bone and Bones; Collagen; Connective Tissue; Contraceptives, Oral, Hormonal; Energy Metabolism; Exercise; Exercise Test; Female; Growth Hormone; Humans; Insulin-Like Growth Factor Binding Proteins; Insulin-Like Growth Factor I; Kinetics; Microdialysis; Muscle, Skeletal; Proline; Tendons; Young Adult

PY - 2009

Y1 - 2009

N2 - Women are at greater risk than men for certain kinds of diseases and injuries, which may at least partly be caused by sex hormonal differences. We aimed to test the influence of estradiol in vivo on collagen synthesis in tendon, bone, and muscle. Two groups of young, healthy women similar in age, body composition, and exercise-training status were included. The two groups were either habitual users of oral contraceptives exposed to a high concentration of synthetic estradiol and progestogens (OC, n = 11), or non-OC-users tested in the follicular phase of the menstrual cycle characterized by low concentrations of estradiol and progesterone (control, n = 12). Subjects performed 1 h of one-legged kicking exercise. The next day collagen fractional synthesis rates (FSR) in tendon and muscle connective tissue were measured after a flooding dose of [(13)C]proline followed by biopsies from the patellar tendon and vastus lateralis in both legs. Simultaneously, microdialysis catheters were inserted in vastus lateralis and in front of the patellar tendon for measurement of insulin-like growth factor I (IGF-I) and its binding proteins. Serum NH(2)-terminal propeptide of type I collagen (PINP) and urine COOH-terminal telopeptides of type-I collagen (CTX-I) were measured as markers for bone synthesis and breakdown, respectively. Tendon FSR and PINP were lower in OC compared with control. An increase in muscle collagen FSR postexercise was only observed in control (P < 0.05). Furthermore, the results indicate a lower bioavailability of IGF-I in OC. In conclusion, synthetic female sex hormones administered as OC had an inhibiting effect on collagen synthesis in tendon, bone, and muscle connective tissue, which may be related to a lower bioavailability of IGF-I.

AB - Women are at greater risk than men for certain kinds of diseases and injuries, which may at least partly be caused by sex hormonal differences. We aimed to test the influence of estradiol in vivo on collagen synthesis in tendon, bone, and muscle. Two groups of young, healthy women similar in age, body composition, and exercise-training status were included. The two groups were either habitual users of oral contraceptives exposed to a high concentration of synthetic estradiol and progestogens (OC, n = 11), or non-OC-users tested in the follicular phase of the menstrual cycle characterized by low concentrations of estradiol and progesterone (control, n = 12). Subjects performed 1 h of one-legged kicking exercise. The next day collagen fractional synthesis rates (FSR) in tendon and muscle connective tissue were measured after a flooding dose of [(13)C]proline followed by biopsies from the patellar tendon and vastus lateralis in both legs. Simultaneously, microdialysis catheters were inserted in vastus lateralis and in front of the patellar tendon for measurement of insulin-like growth factor I (IGF-I) and its binding proteins. Serum NH(2)-terminal propeptide of type I collagen (PINP) and urine COOH-terminal telopeptides of type-I collagen (CTX-I) were measured as markers for bone synthesis and breakdown, respectively. Tendon FSR and PINP were lower in OC compared with control. An increase in muscle collagen FSR postexercise was only observed in control (P < 0.05). Furthermore, the results indicate a lower bioavailability of IGF-I in OC. In conclusion, synthetic female sex hormones administered as OC had an inhibiting effect on collagen synthesis in tendon, bone, and muscle connective tissue, which may be related to a lower bioavailability of IGF-I.

U2 - 10.1152/japplphysiol.90933.2008

DO - 10.1152/japplphysiol.90933.2008

M3 - Journal article

C2 - 18845777

SN - 8750-7587

VL - 106

SP - 1435

EP - 1443

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 4

ER -

Effect of administration of oral contraceptives in vivo on collagen synthesis in tendon and muscle connective tissue in young women

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