Editing modifies the GABA(A) receptor subunit alpha3.

TY - JOUR

T1 - Editing modifies the GABA(A) receptor subunit alpha3.

AU - Ohlson, Johan

AU - Pedersen, Jakob Skou

AU - Haussler, David

AU - Ohman, Marie

N1 - Keywords: Adenosine Deaminase; Animals; Animals, Newborn; Brain; Cells, Cultured; Gene Expression Regulation, Developmental; Humans; Isoleucine; Methionine; Mice; Mice, Inbred Strains; Mice, Mutant Strains; RNA Editing; Receptors, GABA-A

PY - 2007

Y1 - 2007

N2 - Adenosine to inosine (A-to-I) pre-mRNA editing by the ADAR enzyme family has the potential to increase the variety of the proteome. This editing by adenosine deamination is essential in mammals for a functional brain. To detect novel substrates for A-to-I editing we have used an experimental method to find selectively edited sites and combined it with bioinformatic techniques that find stem-loop structures suitable for editing. We present here the first verified editing candidate detected by this screening procedure. We show that Gabra-3, which codes for the alpha3 subunit of the GABA(A) receptor, is a substrate for editing by both ADAR1 and ADAR2. Editing of the Gabra-3 mRNA recodes an isoleucine to a methionine. The extent of editing is low at birth but increases with age, reaching close to 100% in the adult brain. We therefore propose that editing of the Gabra-3 mRNA is important for normal brain development.

AB - Adenosine to inosine (A-to-I) pre-mRNA editing by the ADAR enzyme family has the potential to increase the variety of the proteome. This editing by adenosine deamination is essential in mammals for a functional brain. To detect novel substrates for A-to-I editing we have used an experimental method to find selectively edited sites and combined it with bioinformatic techniques that find stem-loop structures suitable for editing. We present here the first verified editing candidate detected by this screening procedure. We show that Gabra-3, which codes for the alpha3 subunit of the GABA(A) receptor, is a substrate for editing by both ADAR1 and ADAR2. Editing of the Gabra-3 mRNA recodes an isoleucine to a methionine. The extent of editing is low at birth but increases with age, reaching close to 100% in the adult brain. We therefore propose that editing of the Gabra-3 mRNA is important for normal brain development.

U2 - 10.1261/rna.349107

DO - 10.1261/rna.349107

M3 - Journal article

C2 - 17369310

SN - 1355-8382

VL - 13

SP - 698

EP - 703

JO - RNA

JF - RNA

IS - 5

ER -