Dynamics of wnt activity on the acquisition of ectoderm potency in epiblast stem cells

Pierre Osteil; Josh B. Studdert; Hwee Ngee Goh; Emilie E. Wilkie; Xiaochen Fan; Poh Lynn Khoo; Guangdun Peng; Nazmus Salehin; Hilary Knowles; Jing Dong J. Han; Naihe Jing; Nicolas Fossat; Patrick P.L. Tam

doi:10.1242/dev.172858

Dynamics of wnt activity on the acquisition of ectoderm potency in epiblast stem cells

Pierre Osteil^*, Josh B. Studdert, Hwee Ngee Goh, Emilie E. Wilkie, Xiaochen Fan, Poh Lynn Khoo, Guangdun Peng, Nazmus Salehin, Hilary Knowles, Jing Dong J. Han, Naihe Jing, Nicolas Fossat, Patrick P.L. Tam

^*Corresponding author for this work

4 Citations (Scopus)

Abstract

During embryogenesis, the stringent regulation of Wnt activity is crucial for the morphogenesis of the head and brain. The loss of function of the Wnt inhibitor Dkk1 results in elevated Wnt activity, loss of ectoderm lineage attributes from the anterior epiblast, and the posteriorisation of anterior germ layer tissue towards the mesendoderm. The modulation of Wnt signalling may therefore be crucial for the allocation of epiblast cells to ectoderm progenitors during gastrulation. To test this hypothesis, we examined the lineage characteristics of epiblast stem cells (EpiSCs) that were derived and maintained under different signalling conditions. We showed that suppression of Wnt activity enhanced the ectoderm propensity of the EpiSCs. Neuroectoderm differentiation of these EpiSCs was further empowered by the robust re-activation of Wnt activity. Therefore, during gastrulation, the tuning of the signalling activities that mediate mesendoderm differentiation is instrumental for the acquisition of ectoderm potency in the epiblast.

Original language	English
Article number	dev172858
Journal	Development (Cambridge)
Volume	146
Issue number	7
ISSN	0950-1991
DOIs	https://doi.org/10.1242/dev.172858
Publication status	Published - 2019
Externally published	Yes

Keywords

Epiblast stem cells
Head development
Lineage propensity
Neurogenesis
Wnt signalling

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@article{848202998b5c4446890192d26ce76ff1,

title = "Dynamics of wnt activity on the acquisition of ectoderm potency in epiblast stem cells",

abstract = "During embryogenesis, the stringent regulation of Wnt activity is crucial for the morphogenesis of the head and brain. The loss of function of the Wnt inhibitor Dkk1 results in elevated Wnt activity, loss of ectoderm lineage attributes from the anterior epiblast, and the posteriorisation of anterior germ layer tissue towards the mesendoderm. The modulation of Wnt signalling may therefore be crucial for the allocation of epiblast cells to ectoderm progenitors during gastrulation. To test this hypothesis, we examined the lineage characteristics of epiblast stem cells (EpiSCs) that were derived and maintained under different signalling conditions. We showed that suppression of Wnt activity enhanced the ectoderm propensity of the EpiSCs. Neuroectoderm differentiation of these EpiSCs was further empowered by the robust re-activation of Wnt activity. Therefore, during gastrulation, the tuning of the signalling activities that mediate mesendoderm differentiation is instrumental for the acquisition of ectoderm potency in the epiblast.",

keywords = "Epiblast stem cells, Head development, Lineage propensity, Neurogenesis, Wnt signalling",

author = "Pierre Osteil and Studdert, {Josh B.} and Goh, {Hwee Ngee} and Wilkie, {Emilie E.} and Xiaochen Fan and Khoo, {Poh Lynn} and Guangdun Peng and Nazmus Salehin and Hilary Knowles and Han, {Jing Dong J.} and Naihe Jing and Nicolas Fossat and Tam, {Patrick P.L.}",

year = "2019",

doi = "10.1242/dev.172858",

language = "English",

volume = "146",

journal = "Development (Cambridge)",

issn = "0950-1991",

publisher = "The Company of Biologists",

number = "7",

}

TY - JOUR

T1 - Dynamics of wnt activity on the acquisition of ectoderm potency in epiblast stem cells

AU - Osteil, Pierre

AU - Studdert, Josh B.

AU - Goh, Hwee Ngee

AU - Wilkie, Emilie E.

AU - Fan, Xiaochen

AU - Khoo, Poh Lynn

AU - Peng, Guangdun

AU - Salehin, Nazmus

AU - Knowles, Hilary

AU - Han, Jing Dong J.

AU - Jing, Naihe

AU - Fossat, Nicolas

AU - Tam, Patrick P.L.

PY - 2019

Y1 - 2019

N2 - During embryogenesis, the stringent regulation of Wnt activity is crucial for the morphogenesis of the head and brain. The loss of function of the Wnt inhibitor Dkk1 results in elevated Wnt activity, loss of ectoderm lineage attributes from the anterior epiblast, and the posteriorisation of anterior germ layer tissue towards the mesendoderm. The modulation of Wnt signalling may therefore be crucial for the allocation of epiblast cells to ectoderm progenitors during gastrulation. To test this hypothesis, we examined the lineage characteristics of epiblast stem cells (EpiSCs) that were derived and maintained under different signalling conditions. We showed that suppression of Wnt activity enhanced the ectoderm propensity of the EpiSCs. Neuroectoderm differentiation of these EpiSCs was further empowered by the robust re-activation of Wnt activity. Therefore, during gastrulation, the tuning of the signalling activities that mediate mesendoderm differentiation is instrumental for the acquisition of ectoderm potency in the epiblast.

AB - During embryogenesis, the stringent regulation of Wnt activity is crucial for the morphogenesis of the head and brain. The loss of function of the Wnt inhibitor Dkk1 results in elevated Wnt activity, loss of ectoderm lineage attributes from the anterior epiblast, and the posteriorisation of anterior germ layer tissue towards the mesendoderm. The modulation of Wnt signalling may therefore be crucial for the allocation of epiblast cells to ectoderm progenitors during gastrulation. To test this hypothesis, we examined the lineage characteristics of epiblast stem cells (EpiSCs) that were derived and maintained under different signalling conditions. We showed that suppression of Wnt activity enhanced the ectoderm propensity of the EpiSCs. Neuroectoderm differentiation of these EpiSCs was further empowered by the robust re-activation of Wnt activity. Therefore, during gastrulation, the tuning of the signalling activities that mediate mesendoderm differentiation is instrumental for the acquisition of ectoderm potency in the epiblast.

KW - Epiblast stem cells

KW - Head development

KW - Lineage propensity

KW - Neurogenesis

KW - Wnt signalling

U2 - 10.1242/dev.172858

DO - 10.1242/dev.172858

M3 - Journal article

C2 - 30890572

AN - SCOPUS:85064470985

SN - 0950-1991

VL - 146

JO - Development (Cambridge)

JF - Development (Cambridge)

IS - 7

M1 - dev172858

ER -

Dynamics of wnt activity on the acquisition of ectoderm potency in epiblast stem cells

Abstract

Keywords

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