Downregulation of miR-125b in metastatic cutaneous malignant melanoma

Martin Glud; Maria Rossing; Christoffer Hother; Line Holst; Nina Hastrup; Finn Cilius Nielsen; Robert Gniadecki; Krzysztof T Drzewiecki

doi:10.1097/cmr.0b013e32833e32a1

Downregulation of miR-125b in metastatic cutaneous malignant melanoma

Martin Glud, Maria Rossing, Christoffer Hother, Line Holst, Nina Hastrup, Finn Cilius Nielsen, Robert Gniadecki, Krzysztof T Drzewiecki

62 Citations (Scopus)

Abstract

This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM.

Original language	English
Journal	Melanoma Research
Volume	20
Issue number	6
Pages (from-to)	479-84
Number of pages	6
ISSN	0960-8931
DOIs	https://doi.org/10.1097/cmr.0b013e32833e32a1
Publication status	Published - 1 Dec 2010

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title = "Downregulation of miR-125b in metastatic cutaneous malignant melanoma",

abstract = "This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM.",

author = "Martin Glud and Maria Rossing and Christoffer Hother and Line Holst and Nina Hastrup and Nielsen, {Finn Cilius} and Robert Gniadecki and Drzewiecki, {Krzysztof T}",

year = "2010",

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doi = "10.1097/cmr.0b013e32833e32a1",

language = "English",

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pages = "479--84",

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TY - JOUR

T1 - Downregulation of miR-125b in metastatic cutaneous malignant melanoma

AU - Glud, Martin

AU - Rossing, Maria

AU - Hother, Christoffer

AU - Holst, Line

AU - Hastrup, Nina

AU - Nielsen, Finn Cilius

AU - Gniadecki, Robert

AU - Drzewiecki, Krzysztof T

PY - 2010/12/1

Y1 - 2010/12/1

N2 - This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM.

AB - This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM.

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DO - 10.1097/cmr.0b013e32833e32a1

M3 - Journal article

SN - 0960-8931

VL - 20

SP - 479

EP - 484

JO - Melanoma Research

JF - Melanoma Research

IS - 6

ER -

Downregulation of miR-125b in metastatic cutaneous malignant melanoma

Abstract

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