Dominant negative autoregulation limits steady-state repression levels in gene networks

Szabolcs Semsey; Sandeep Krishna; János Erdossy; Péter Horváth; László Orosz; Kim Sneppen; Sankar Adhya

doi:10.1128/JB.00056-09

Dominant negative autoregulation limits steady-state repression levels in gene networks

Szabolcs Semsey, Sandeep Krishna, János Erdossy, Péter Horváth, László Orosz, Kim Sneppen, Sankar Adhya

16 Citations (Scopus)

Abstract

Many transcription factors repress transcription of their own genes. Negative autoregulation has been shown to reduce cell-cell variation in regulatory protein levels and speed up the response time in gene networks. In this work we examined transcription regulation of the galS gene and the function of its product, the GalS protein. We observed a unique operator preference of the GalS protein characterized by dominant negative autoregulation. We show that this pattern of regulation limits the repression level of the target genes in steady states. We suggest that transcription factors with dominant negative autoregulation are designed for regulating gene expression during environmental transitions.

Original language	English
Journal	Journal of Bacteriology
Volume	191
Issue number	14
Pages (from-to)	4487-91
Number of pages	5
ISSN	0021-9193
DOIs	https://doi.org/10.1128/JB.00056-09
Publication status	Published - Jul 2009
Externally published	Yes

Keywords

Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation
Gene Regulatory Networks
Homeostasis
Repressor Proteins
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

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title = "Dominant negative autoregulation limits steady-state repression levels in gene networks",

abstract = "Many transcription factors repress transcription of their own genes. Negative autoregulation has been shown to reduce cell-cell variation in regulatory protein levels and speed up the response time in gene networks. In this work we examined transcription regulation of the galS gene and the function of its product, the GalS protein. We observed a unique operator preference of the GalS protein characterized by dominant negative autoregulation. We show that this pattern of regulation limits the repression level of the target genes in steady states. We suggest that transcription factors with dominant negative autoregulation are designed for regulating gene expression during environmental transitions.",

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year = "2009",

month = jul,

doi = "10.1128/JB.00056-09",

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volume = "191",

pages = "4487--91",

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publisher = "American Society for Microbiology",

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T1 - Dominant negative autoregulation limits steady-state repression levels in gene networks

AU - Semsey, Szabolcs

AU - Krishna, Sandeep

AU - Erdossy, János

AU - Horváth, Péter

AU - Orosz, László

AU - Sneppen, Kim

AU - Adhya, Sankar

PY - 2009/7

Y1 - 2009/7

N2 - Many transcription factors repress transcription of their own genes. Negative autoregulation has been shown to reduce cell-cell variation in regulatory protein levels and speed up the response time in gene networks. In this work we examined transcription regulation of the galS gene and the function of its product, the GalS protein. We observed a unique operator preference of the GalS protein characterized by dominant negative autoregulation. We show that this pattern of regulation limits the repression level of the target genes in steady states. We suggest that transcription factors with dominant negative autoregulation are designed for regulating gene expression during environmental transitions.

AB - Many transcription factors repress transcription of their own genes. Negative autoregulation has been shown to reduce cell-cell variation in regulatory protein levels and speed up the response time in gene networks. In this work we examined transcription regulation of the galS gene and the function of its product, the GalS protein. We observed a unique operator preference of the GalS protein characterized by dominant negative autoregulation. We show that this pattern of regulation limits the repression level of the target genes in steady states. We suggest that transcription factors with dominant negative autoregulation are designed for regulating gene expression during environmental transitions.

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Gene Expression Regulation

KW - Gene Regulatory Networks

KW - Homeostasis

KW - Repressor Proteins

KW - Journal Article

KW - Research Support, N.I.H., Intramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1128/JB.00056-09

DO - 10.1128/JB.00056-09

M3 - Journal article

C2 - 19429616

SN - 0021-9193

VL - 191

SP - 4487

EP - 4491

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 14

ER -

Dominant negative autoregulation limits steady-state repression levels in gene networks

Abstract

Keywords

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