DNA vaccines encoding proteins from wild-type and attenuated canine distemper virus protect equally well against wild-type virus challenge

Line Nielsen; Trine Hammer Jensen; Birte Kristensen; Tove Johanne Dannemann Jensen; Peter Karlskov-Mortensen; Morten Lund; Bent Aasted; Merete Blixenkrone-Møller

doi:10.1007/s00705-012-1375-y

DNA vaccines encoding proteins from wild-type and attenuated canine distemper virus protect equally well against wild-type virus challenge

Line Nielsen, Trine Hammer Jensen, Birte Kristensen, Tove Johanne Dannemann Jensen, Peter Karlskov-Mortensen, Morten Lund, Bent Aasted, Merete Blixenkrone-Møller

Department of Immunology and Microbiology

5 Citations (Scopus)

Abstract

Immunity induced by DNA vaccines containing the hemagglutinin (H) and nucleoprotein (N) genes of wild-type and attenuated canine distemper virus (CDV) was investigated in mink (Mustela vison), a highly susceptible natural host of CDV. All DNA-immunized mink seroconverted, and significant levels of virus-neutralizing (VN) antibodies were present on the day of challenge with wild-type CDV. The DNA vaccines also primed the cell-mediated memory responses, as indicated by an early increase in the number of interferon-gamma (IFN-γ)-producing lymphocytes after challenge. Importantly, the wild-type and attenuated CDV DNA vaccines had a long-term protective effect against wild-type CDV challenge. The vaccine-induced immunity induced by the H and N genes from wild-type CDV and those from attenuated CDV was comparable. Because these two DNA vaccines were shown to protect equally well against wild-type virus challenge, it is suggested that the genetic/antigenic heterogeneity between vaccine strains and contemporary wild-type strains are unlikely to cause vaccine failure.

Original language	English
Journal	Archives of Virology
Volume	157
Issue number	10
Pages (from-to)	1887-1896
Number of pages	10
ISSN	0304-8608
DOIs	https://doi.org/10.1007/s00705-012-1375-y
Publication status	Published - Oct 2012

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10.1007/s00705-012-1375-y

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@article{66dc2186f953473e85cad9cccc8ea29b,

title = "DNA vaccines encoding proteins from wild-type and attenuated canine distemper virus protect equally well against wild-type virus challenge",

abstract = "Immunity induced by DNA vaccines containing the hemagglutinin (H) and nucleoprotein (N) genes of wild-type and attenuated canine distemper virus (CDV) was investigated in mink (Mustela vison), a highly susceptible natural host of CDV. All DNA-immunized mink seroconverted, and significant levels of virus-neutralizing (VN) antibodies were present on the day of challenge with wild-type CDV. The DNA vaccines also primed the cell-mediated memory responses, as indicated by an early increase in the number of interferon-gamma (IFN-γ)-producing lymphocytes after challenge. Importantly, the wild-type and attenuated CDV DNA vaccines had a long-term protective effect against wild-type CDV challenge. The vaccine-induced immunity induced by the H and N genes from wild-type CDV and those from attenuated CDV was comparable. Because these two DNA vaccines were shown to protect equally well against wild-type virus challenge, it is suggested that the genetic/antigenic heterogeneity between vaccine strains and contemporary wild-type strains are unlikely to cause vaccine failure.",

author = "Line Nielsen and Jensen, {Trine Hammer} and Birte Kristensen and Jensen, {Tove Johanne Dannemann} and Peter Karlskov-Mortensen and Morten Lund and Bent Aasted and Merete Blixenkrone-M{\o}ller",

year = "2012",

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doi = "10.1007/s00705-012-1375-y",

language = "English",

volume = "157",

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T1 - DNA vaccines encoding proteins from wild-type and attenuated canine distemper virus protect equally well against wild-type virus challenge

AU - Nielsen, Line

AU - Jensen, Trine Hammer

AU - Kristensen, Birte

AU - Jensen, Tove Johanne Dannemann

AU - Karlskov-Mortensen, Peter

AU - Lund, Morten

AU - Aasted, Bent

AU - Blixenkrone-Møller, Merete

PY - 2012/10

Y1 - 2012/10

N2 - Immunity induced by DNA vaccines containing the hemagglutinin (H) and nucleoprotein (N) genes of wild-type and attenuated canine distemper virus (CDV) was investigated in mink (Mustela vison), a highly susceptible natural host of CDV. All DNA-immunized mink seroconverted, and significant levels of virus-neutralizing (VN) antibodies were present on the day of challenge with wild-type CDV. The DNA vaccines also primed the cell-mediated memory responses, as indicated by an early increase in the number of interferon-gamma (IFN-γ)-producing lymphocytes after challenge. Importantly, the wild-type and attenuated CDV DNA vaccines had a long-term protective effect against wild-type CDV challenge. The vaccine-induced immunity induced by the H and N genes from wild-type CDV and those from attenuated CDV was comparable. Because these two DNA vaccines were shown to protect equally well against wild-type virus challenge, it is suggested that the genetic/antigenic heterogeneity between vaccine strains and contemporary wild-type strains are unlikely to cause vaccine failure.

AB - Immunity induced by DNA vaccines containing the hemagglutinin (H) and nucleoprotein (N) genes of wild-type and attenuated canine distemper virus (CDV) was investigated in mink (Mustela vison), a highly susceptible natural host of CDV. All DNA-immunized mink seroconverted, and significant levels of virus-neutralizing (VN) antibodies were present on the day of challenge with wild-type CDV. The DNA vaccines also primed the cell-mediated memory responses, as indicated by an early increase in the number of interferon-gamma (IFN-γ)-producing lymphocytes after challenge. Importantly, the wild-type and attenuated CDV DNA vaccines had a long-term protective effect against wild-type CDV challenge. The vaccine-induced immunity induced by the H and N genes from wild-type CDV and those from attenuated CDV was comparable. Because these two DNA vaccines were shown to protect equally well against wild-type virus challenge, it is suggested that the genetic/antigenic heterogeneity between vaccine strains and contemporary wild-type strains are unlikely to cause vaccine failure.

U2 - 10.1007/s00705-012-1375-y

DO - 10.1007/s00705-012-1375-y

M3 - Journal article

C2 - 22714870

SN - 0304-8608

VL - 157

SP - 1887

EP - 1896

JO - Archiv fur die gesamte Virusforschung

JF - Archiv fur die gesamte Virusforschung

IS - 10

ER -

DNA vaccines encoding proteins from wild-type and attenuated canine distemper virus protect equally well against wild-type virus challenge

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