DNA extraction from keratin and chitin

Paula F. Campos, M. Thomas P. Gilbert*

*Corresponding author for this work
    1 Citation (Scopus)

    Abstract

    DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica column or organic methods.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    Number of pages7
    PublisherHumana Press
    Publication date1 Jan 2019
    Pages57-63
    DOIs
    Publication statusPublished - 1 Jan 2019
    SeriesMethods in Molecular Biology
    Volume1963
    ISSN1064-3745

    Keywords

    • Ancient DNA
    • Chitinous tissue
    • Cuticle
    • DNA extraction
    • Exoskeleton
    • Feathers
    • Hair
    • Keratinous tissue
    • Nail
    • Silica

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