Diurnal variation of urinary markers of nucleic acid oxidation

Ida S Grew; Vanja Cejvanovic; Kasper Broedbaek; Trine Henriksen; Morten Petersen; Jon T Andersen; Espen Jimenez-Solem; Allan Weimann; Henrik E Poulsen

doi:10.3109/00365513.2014.891258

Diurnal variation of urinary markers of nucleic acid oxidation

Ida S Grew, Vanja Cejvanovic, Kasper Broedbaek, Trine Henriksen, Morten Petersen, Jon T Andersen, Espen Jimenez-Solem, Allan Weimann, Henrik E Poulsen

10 Citations (Scopus)

Abstract

AIMS: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) are biomarkers of oxidative stress with clinical potential in a variety of diseases. As part of their clinical validation, this study aimed to investigate whether the urinary excretion of 8-oxodG and 8-oxoGuo undergoes diurnal variation and to evaluate the validity of 6-hour sampling as well as creatinine corrected spot urine sampling.

METHODS: A total of 23 healthy study subjects collecting their 24-h urine in four fractions covering 6 hours each. Urinary 8-oxodG and 8-oxoGuo levels were quantified using a modified version of UPLC-MS/MS.

RESULTS: No significant difference in excretion levels between the 12-h diurnal and 12-h nocturnal state or between the four 6-h periods during the day was found for either biomarker. A strong linear relationship between the excretion levels in each of the 6-h periods and the 24-h excretion level was shown for both biomarkers. Creatinine correction of the 6-h levels reduced the biological variation of the excretion levels and weakened the linear relationship with the uncorrected 24-h excretion level for both biomarkers. The correlations were strengthened when the 24-h excretion level was expressed per kg body weight.

CONCLUSION: The results showed that 8-oxodG and 8-oxoGuo did not undergo diurnal variation in the study population overall and hence that the time of sampling is not crucial. Furthermore, 6-h sampling can be used as a substitute for 24-h sampling, and creatinine corrected sampling may be rational due to the reduction in biological variation of the biomarkers and the reasonable correlation with body weight-adjusted 24-h levels.

Original language	English
Journal	Scandinavian Journal of Clinical & Laboratory Investigation
Volume	74
Issue number	4
Pages (from-to)	336-343
Number of pages	8
ISSN	0036-5513
DOIs	https://doi.org/10.3109/00365513.2014.891258
Publication status	Published - Jun 2014

Keywords

Adult
Aged
Biological Markers
Circadian Rhythm
Creatinine
Deoxyguanosine
Female
Guanosine
Humans
Male
Middle Aged
Oxidation-Reduction
Oxidative Stress
Reproducibility of Results

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10.3109/00365513.2014.891258

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@article{84d4198cec5f41d2846902ec72189bbe,

title = "Diurnal variation of urinary markers of nucleic acid oxidation",

abstract = "AIMS: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) are biomarkers of oxidative stress with clinical potential in a variety of diseases. As part of their clinical validation, this study aimed to investigate whether the urinary excretion of 8-oxodG and 8-oxoGuo undergoes diurnal variation and to evaluate the validity of 6-hour sampling as well as creatinine corrected spot urine sampling.METHODS: A total of 23 healthy study subjects collecting their 24-h urine in four fractions covering 6 hours each. Urinary 8-oxodG and 8-oxoGuo levels were quantified using a modified version of UPLC-MS/MS.RESULTS: No significant difference in excretion levels between the 12-h diurnal and 12-h nocturnal state or between the four 6-h periods during the day was found for either biomarker. A strong linear relationship between the excretion levels in each of the 6-h periods and the 24-h excretion level was shown for both biomarkers. Creatinine correction of the 6-h levels reduced the biological variation of the excretion levels and weakened the linear relationship with the uncorrected 24-h excretion level for both biomarkers. The correlations were strengthened when the 24-h excretion level was expressed per kg body weight.CONCLUSION: The results showed that 8-oxodG and 8-oxoGuo did not undergo diurnal variation in the study population overall and hence that the time of sampling is not crucial. Furthermore, 6-h sampling can be used as a substitute for 24-h sampling, and creatinine corrected sampling may be rational due to the reduction in biological variation of the biomarkers and the reasonable correlation with body weight-adjusted 24-h levels.",

keywords = "Adult, Aged, Biological Markers, Circadian Rhythm, Creatinine, Deoxyguanosine, Female, Guanosine, Humans, Male, Middle Aged, Oxidation-Reduction, Oxidative Stress, Reproducibility of Results",

author = "Grew, {Ida S} and Vanja Cejvanovic and Kasper Broedbaek and Trine Henriksen and Morten Petersen and Andersen, {Jon T} and Espen Jimenez-Solem and Allan Weimann and Poulsen, {Henrik E}",

year = "2014",

month = jun,

doi = "10.3109/00365513.2014.891258",

language = "English",

volume = "74",

pages = "336--343",

journal = "Scandinavian Journal of Clinical & Laboratory Investigation",

issn = "0036-5513",

publisher = "Taylor & Francis",

number = "4",

}

TY - JOUR

T1 - Diurnal variation of urinary markers of nucleic acid oxidation

AU - Grew, Ida S

AU - Cejvanovic, Vanja

AU - Broedbaek, Kasper

AU - Henriksen, Trine

AU - Petersen, Morten

AU - Andersen, Jon T

AU - Jimenez-Solem, Espen

AU - Weimann, Allan

AU - Poulsen, Henrik E

PY - 2014/6

Y1 - 2014/6

N2 - AIMS: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) are biomarkers of oxidative stress with clinical potential in a variety of diseases. As part of their clinical validation, this study aimed to investigate whether the urinary excretion of 8-oxodG and 8-oxoGuo undergoes diurnal variation and to evaluate the validity of 6-hour sampling as well as creatinine corrected spot urine sampling.METHODS: A total of 23 healthy study subjects collecting their 24-h urine in four fractions covering 6 hours each. Urinary 8-oxodG and 8-oxoGuo levels were quantified using a modified version of UPLC-MS/MS.RESULTS: No significant difference in excretion levels between the 12-h diurnal and 12-h nocturnal state or between the four 6-h periods during the day was found for either biomarker. A strong linear relationship between the excretion levels in each of the 6-h periods and the 24-h excretion level was shown for both biomarkers. Creatinine correction of the 6-h levels reduced the biological variation of the excretion levels and weakened the linear relationship with the uncorrected 24-h excretion level for both biomarkers. The correlations were strengthened when the 24-h excretion level was expressed per kg body weight.CONCLUSION: The results showed that 8-oxodG and 8-oxoGuo did not undergo diurnal variation in the study population overall and hence that the time of sampling is not crucial. Furthermore, 6-h sampling can be used as a substitute for 24-h sampling, and creatinine corrected sampling may be rational due to the reduction in biological variation of the biomarkers and the reasonable correlation with body weight-adjusted 24-h levels.

AB - AIMS: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) are biomarkers of oxidative stress with clinical potential in a variety of diseases. As part of their clinical validation, this study aimed to investigate whether the urinary excretion of 8-oxodG and 8-oxoGuo undergoes diurnal variation and to evaluate the validity of 6-hour sampling as well as creatinine corrected spot urine sampling.METHODS: A total of 23 healthy study subjects collecting their 24-h urine in four fractions covering 6 hours each. Urinary 8-oxodG and 8-oxoGuo levels were quantified using a modified version of UPLC-MS/MS.RESULTS: No significant difference in excretion levels between the 12-h diurnal and 12-h nocturnal state or between the four 6-h periods during the day was found for either biomarker. A strong linear relationship between the excretion levels in each of the 6-h periods and the 24-h excretion level was shown for both biomarkers. Creatinine correction of the 6-h levels reduced the biological variation of the excretion levels and weakened the linear relationship with the uncorrected 24-h excretion level for both biomarkers. The correlations were strengthened when the 24-h excretion level was expressed per kg body weight.CONCLUSION: The results showed that 8-oxodG and 8-oxoGuo did not undergo diurnal variation in the study population overall and hence that the time of sampling is not crucial. Furthermore, 6-h sampling can be used as a substitute for 24-h sampling, and creatinine corrected sampling may be rational due to the reduction in biological variation of the biomarkers and the reasonable correlation with body weight-adjusted 24-h levels.

KW - Adult

KW - Aged

KW - Biological Markers

KW - Circadian Rhythm

KW - Creatinine

KW - Deoxyguanosine

KW - Female

KW - Guanosine

KW - Humans

KW - Male

KW - Middle Aged

KW - Oxidation-Reduction

KW - Oxidative Stress

KW - Reproducibility of Results

U2 - 10.3109/00365513.2014.891258

DO - 10.3109/00365513.2014.891258

M3 - Journal article

C2 - 24628455

SN - 0036-5513

VL - 74

SP - 336

EP - 343

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

IS - 4

ER -

Diurnal variation of urinary markers of nucleic acid oxidation

Abstract

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