Diurnal variation of hematology parameters in healthy young males: the Bispebjerg study of diurnal variations

Henriette P Sennels; Henrik L Jørgensen; Anne-Louise S Hansen; Jens P Goetze; Jan Fahrenkrug

doi:10.3109/00365513.2011.602422

Diurnal variation of hematology parameters in healthy young males: the Bispebjerg study of diurnal variations

Henriette P Sennels, Henrik L Jørgensen, Anne-Louise S Hansen, Jens P Goetze, Jan Fahrenkrug

90 Citations (Scopus)

Abstract

Purpose. To evaluate the influence of time of day on the circulating concentrations of 21 hematology parameters. Materials and methods. Venous blood samples were obtained under standardized circumstances from 24 healthy young men every third hour through 24 hours, nine time points in total. At each time point, the level of melatonin, iron, transferrin, transferrin saturation, ferritin, cobalamin, folate, red blood cells and white blood cells was measured. The data were analysed by rhythmometric statistical methods. The biological variations were calculated. Results. Significant oscillation of melatonin (p < 0.0001) with an amplitude (amp) of 19.84 pg/ml and a peak level at 03:34 h confirmed the normal 24-hour rhythms of the participants. Erythrocytes (p < 0.0001, amp = 0.15 × 10 ¹²/L), hemoglobin (p < 0.0001, amp = 0.29 mmol/L), hematocrit (p < 0.0001, amp = 0.01), iron (p < 0.0001, amp = 4.00μmol/L), transferrin (p = 0.03, amp = 1.41μmol/L), transferrin saturation (p < 0.0001, amp = 6.37%) and folate (p < 0.0001, amp = 1.55nmol/L) oscillated significantly, with gradually falling mean levels through the day to nadir around midnight. Leukocyte count (p < 0.0001, amp = 0.78 × 10 ⁹/L), neutrophils (p = 0.001, 0.31 × 10 ⁹/L), eosinophils (p < 0.0001, amp = 0.04 × 10 ⁹/L), monocytes (p = 0.0009, amp = 0.06 × 10 ⁹/L), lymphocytes (p < 0.0001, amp = 0.49 × 10 ⁹/L) oscillated significantly with gradually increasing mean levels through the day peaking at midnight. Iron, leukocytes and hemoglobin had the highest 24 hour oscillations in proportion to the reference intervals of the parameters for healthy young men. Conclusions. Biochemical screenings are biased by diurnal variations, which must be considered when blood concentrations of these parameters are interpreted in the clinical setting.

Original language	English
Journal	Scandinavian Journal of Clinical & Laboratory Investigation
Volume	71
Issue number	7
Pages (from-to)	532-41
Number of pages	10
ISSN	0036-5513
DOIs	https://doi.org/10.3109/00365513.2011.602422
Publication status	Published - Nov 2011

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Diurnal variation of hematology parameters in healthy young males: the Bispebjerg study of diurnal variations. / Sennels, Henriette P; Jørgensen, Henrik L; Hansen, Anne-Louise S et al.
In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 71, No. 7, 11.2011, p. 532-41.

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title = "Diurnal variation of hematology parameters in healthy young males: the Bispebjerg study of diurnal variations",

abstract = "Purpose. To evaluate the influence of time of day on the circulating concentrations of 21 hematology parameters. Materials and methods. Venous blood samples were obtained under standardized circumstances from 24 healthy young men every third hour through 24 hours, nine time points in total. At each time point, the level of melatonin, iron, transferrin, transferrin saturation, ferritin, cobalamin, folate, red blood cells and white blood cells was measured. The data were analysed by rhythmometric statistical methods. The biological variations were calculated. Results. Significant oscillation of melatonin (p < 0.0001) with an amplitude (amp) of 19.84 pg/ml and a peak level at 03:34 h confirmed the normal 24-hour rhythms of the participants. Erythrocytes (p < 0.0001, amp = 0.15 × 10 12/L), hemoglobin (p < 0.0001, amp = 0.29 mmol/L), hematocrit (p < 0.0001, amp = 0.01), iron (p < 0.0001, amp = 4.00μmol/L), transferrin (p = 0.03, amp = 1.41μmol/L), transferrin saturation (p < 0.0001, amp = 6.37%) and folate (p < 0.0001, amp = 1.55nmol/L) oscillated significantly, with gradually falling mean levels through the day to nadir around midnight. Leukocyte count (p < 0.0001, amp = 0.78 × 10 9/L), neutrophils (p = 0.001, 0.31 × 10 9/L), eosinophils (p < 0.0001, amp = 0.04 × 10 9/L), monocytes (p = 0.0009, amp = 0.06 × 10 9/L), lymphocytes (p < 0.0001, amp = 0.49 × 10 9/L) oscillated significantly with gradually increasing mean levels through the day peaking at midnight. Iron, leukocytes and hemoglobin had the highest 24 hour oscillations in proportion to the reference intervals of the parameters for healthy young men. Conclusions. Biochemical screenings are biased by diurnal variations, which must be considered when blood concentrations of these parameters are interpreted in the clinical setting.",

author = "Sennels, {Henriette P} and J{\o}rgensen, {Henrik L} and Hansen, {Anne-Louise S} and Goetze, {Jens P} and Jan Fahrenkrug",

year = "2011",

month = nov,

doi = "10.3109/00365513.2011.602422",

language = "English",

volume = "71",

pages = "532--41",

journal = "Scandinavian Journal of Clinical & Laboratory Investigation",

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T1 - Diurnal variation of hematology parameters in healthy young males: the Bispebjerg study of diurnal variations

AU - Sennels, Henriette P

AU - Jørgensen, Henrik L

AU - Hansen, Anne-Louise S

AU - Goetze, Jens P

AU - Fahrenkrug, Jan

PY - 2011/11

Y1 - 2011/11

N2 - Purpose. To evaluate the influence of time of day on the circulating concentrations of 21 hematology parameters. Materials and methods. Venous blood samples were obtained under standardized circumstances from 24 healthy young men every third hour through 24 hours, nine time points in total. At each time point, the level of melatonin, iron, transferrin, transferrin saturation, ferritin, cobalamin, folate, red blood cells and white blood cells was measured. The data were analysed by rhythmometric statistical methods. The biological variations were calculated. Results. Significant oscillation of melatonin (p < 0.0001) with an amplitude (amp) of 19.84 pg/ml and a peak level at 03:34 h confirmed the normal 24-hour rhythms of the participants. Erythrocytes (p < 0.0001, amp = 0.15 × 10 12/L), hemoglobin (p < 0.0001, amp = 0.29 mmol/L), hematocrit (p < 0.0001, amp = 0.01), iron (p < 0.0001, amp = 4.00μmol/L), transferrin (p = 0.03, amp = 1.41μmol/L), transferrin saturation (p < 0.0001, amp = 6.37%) and folate (p < 0.0001, amp = 1.55nmol/L) oscillated significantly, with gradually falling mean levels through the day to nadir around midnight. Leukocyte count (p < 0.0001, amp = 0.78 × 10 9/L), neutrophils (p = 0.001, 0.31 × 10 9/L), eosinophils (p < 0.0001, amp = 0.04 × 10 9/L), monocytes (p = 0.0009, amp = 0.06 × 10 9/L), lymphocytes (p < 0.0001, amp = 0.49 × 10 9/L) oscillated significantly with gradually increasing mean levels through the day peaking at midnight. Iron, leukocytes and hemoglobin had the highest 24 hour oscillations in proportion to the reference intervals of the parameters for healthy young men. Conclusions. Biochemical screenings are biased by diurnal variations, which must be considered when blood concentrations of these parameters are interpreted in the clinical setting.

AB - Purpose. To evaluate the influence of time of day on the circulating concentrations of 21 hematology parameters. Materials and methods. Venous blood samples were obtained under standardized circumstances from 24 healthy young men every third hour through 24 hours, nine time points in total. At each time point, the level of melatonin, iron, transferrin, transferrin saturation, ferritin, cobalamin, folate, red blood cells and white blood cells was measured. The data were analysed by rhythmometric statistical methods. The biological variations were calculated. Results. Significant oscillation of melatonin (p < 0.0001) with an amplitude (amp) of 19.84 pg/ml and a peak level at 03:34 h confirmed the normal 24-hour rhythms of the participants. Erythrocytes (p < 0.0001, amp = 0.15 × 10 12/L), hemoglobin (p < 0.0001, amp = 0.29 mmol/L), hematocrit (p < 0.0001, amp = 0.01), iron (p < 0.0001, amp = 4.00μmol/L), transferrin (p = 0.03, amp = 1.41μmol/L), transferrin saturation (p < 0.0001, amp = 6.37%) and folate (p < 0.0001, amp = 1.55nmol/L) oscillated significantly, with gradually falling mean levels through the day to nadir around midnight. Leukocyte count (p < 0.0001, amp = 0.78 × 10 9/L), neutrophils (p = 0.001, 0.31 × 10 9/L), eosinophils (p < 0.0001, amp = 0.04 × 10 9/L), monocytes (p = 0.0009, amp = 0.06 × 10 9/L), lymphocytes (p < 0.0001, amp = 0.49 × 10 9/L) oscillated significantly with gradually increasing mean levels through the day peaking at midnight. Iron, leukocytes and hemoglobin had the highest 24 hour oscillations in proportion to the reference intervals of the parameters for healthy young men. Conclusions. Biochemical screenings are biased by diurnal variations, which must be considered when blood concentrations of these parameters are interpreted in the clinical setting.

U2 - 10.3109/00365513.2011.602422

DO - 10.3109/00365513.2011.602422

M3 - Journal article

SN - 0036-5513

VL - 71

SP - 532

EP - 541

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

IS - 7

ER -

Diurnal variation of hematology parameters in healthy young males: the Bispebjerg study of diurnal variations

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