Distribution of three microvillar enzymes along the small intestinal crypt-villus axis

Gert Helge Hansen; L L Niels-Christiansen; M D Poulsen; Ove Norén; H Sjöström

Distribution of three microvillar enzymes along the small intestinal crypt-villus axis

Gert Helge Hansen, L L Niels-Christiansen, M D Poulsen, Ove Norén, H Sjöström

14 Citations (Scopus)

Abstract

Aminopeptidase N (ApN), dipeptidyl peptidase IV (DPP IV) and sucrose-isomaltase (S-I) are differentially expressed along the pig jejunum crypt-villus axis. Quantitative immunoelectron microscopy and enzyme cytochemistry show that DPP IV and S-I are expressed in enterocytes along the entire length of the axis, whereas ApN is found mainly in the villar and upper crypt enterocytes. All three enzymes are detected in the basolateral membrane at all levels along the crypt-villus axis, although ApN and S-I only occurred at low intensities in the villus region. The microvillar/basolateral labelling ratio for the three enzymes increases to a varying degree for the three enzymes along the axis suggesting that the sorting efficiency to the apical membrane improves at least for ApN and S-I as the cells mature. These findings might indicate that the enterocytes change from a transcytotic to a direct apical transport as the enterocytes mature.

Original language	English
Journal	Journal of Submicroscopic Cytology and Pathology
Volume	26
Issue number	4
Pages (from-to)	453-460
Number of pages	7
ISSN	1122-9497
Publication status	Published - 1994

Cite this

@article{7b5ed8a074ce11dbbee902004c4f4f50,

title = "Distribution of three microvillar enzymes along the small intestinal crypt-villus axis",

abstract = "Aminopeptidase N (ApN), dipeptidyl peptidase IV (DPP IV) and sucrose-isomaltase (S-I) are differentially expressed along the pig jejunum crypt-villus axis. Quantitative immunoelectron microscopy and enzyme cytochemistry show that DPP IV and S-I are expressed in enterocytes along the entire length of the axis, whereas ApN is found mainly in the villar and upper crypt enterocytes. All three enzymes are detected in the basolateral membrane at all levels along the crypt-villus axis, although ApN and S-I only occurred at low intensities in the villus region. The microvillar/basolateral labelling ratio for the three enzymes increases to a varying degree for the three enzymes along the axis suggesting that the sorting efficiency to the apical membrane improves at least for ApN and S-I as the cells mature. These findings might indicate that the enterocytes change from a transcytotic to a direct apical transport as the enterocytes mature.",

author = "Hansen, {Gert Helge} and Niels-Christiansen, {L L} and Poulsen, {M D} and Ove Nor{\'e}n and H Sj{\"o}str{\"o}m",

note = "Keywords: Animals; Antigens, CD13; Antigens, CD26; Immunohistochemistry; Intestine, Small; Isomaltose; Microscopy, Electron; Swine",

year = "1994",

language = "English",

volume = "26",

pages = "453--460",

journal = "Journal of Submicroscopic Cytology and Pathology",

issn = "1122-9497",

publisher = "Nuova Immagine Editrice (N I E)",

number = "4",

}

TY - JOUR

T1 - Distribution of three microvillar enzymes along the small intestinal crypt-villus axis

AU - Hansen, Gert Helge

AU - Niels-Christiansen, L L

AU - Poulsen, M D

AU - Norén, Ove

AU - Sjöström, H

N1 - Keywords: Animals; Antigens, CD13; Antigens, CD26; Immunohistochemistry; Intestine, Small; Isomaltose; Microscopy, Electron; Swine

PY - 1994

Y1 - 1994

N2 - Aminopeptidase N (ApN), dipeptidyl peptidase IV (DPP IV) and sucrose-isomaltase (S-I) are differentially expressed along the pig jejunum crypt-villus axis. Quantitative immunoelectron microscopy and enzyme cytochemistry show that DPP IV and S-I are expressed in enterocytes along the entire length of the axis, whereas ApN is found mainly in the villar and upper crypt enterocytes. All three enzymes are detected in the basolateral membrane at all levels along the crypt-villus axis, although ApN and S-I only occurred at low intensities in the villus region. The microvillar/basolateral labelling ratio for the three enzymes increases to a varying degree for the three enzymes along the axis suggesting that the sorting efficiency to the apical membrane improves at least for ApN and S-I as the cells mature. These findings might indicate that the enterocytes change from a transcytotic to a direct apical transport as the enterocytes mature.

AB - Aminopeptidase N (ApN), dipeptidyl peptidase IV (DPP IV) and sucrose-isomaltase (S-I) are differentially expressed along the pig jejunum crypt-villus axis. Quantitative immunoelectron microscopy and enzyme cytochemistry show that DPP IV and S-I are expressed in enterocytes along the entire length of the axis, whereas ApN is found mainly in the villar and upper crypt enterocytes. All three enzymes are detected in the basolateral membrane at all levels along the crypt-villus axis, although ApN and S-I only occurred at low intensities in the villus region. The microvillar/basolateral labelling ratio for the three enzymes increases to a varying degree for the three enzymes along the axis suggesting that the sorting efficiency to the apical membrane improves at least for ApN and S-I as the cells mature. These findings might indicate that the enterocytes change from a transcytotic to a direct apical transport as the enterocytes mature.

M3 - Journal article

C2 - 7820811

SN - 1122-9497

VL - 26

SP - 453

EP - 460

JO - Journal of Submicroscopic Cytology and Pathology

JF - Journal of Submicroscopic Cytology and Pathology

IS - 4

ER -

Distribution of three microvillar enzymes along the small intestinal crypt-villus axis

Abstract

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