Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism

Shiraz Ali Shah; Niels R Hansen; Roger A Garrett

doi:10.1042/BST0370023

Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism

Shiraz Ali Shah, Niels R Hansen, Roger A Garrett

Functional Genomics

77 Citations (Scopus)

Abstract

Transcripts from spacer sequences within chromosomal repeat clusters [CRISPRs (clusters of regularly interspaced palindromic repeats)] from archaea have been implicated in inhibiting or regulating the propagation of archaeal viruses and plasmids. For the crenarchaeal thermoacidophiles, the chromosomal spacers show a high level of matches ( approximately 30%) with viral or plasmid genomes. Moreover, their distribution along the virus/plasmid genomes, as well as their DNA strand specificity, appear to be random. This is consistent with the hypothesis that chromosomal spacers are taken up directly and randomly from virus and plasmid DNA and that the spacer transcripts target the genomic DNA of the extrachromosomal elements and not their transcripts.

Original language	English
Journal	Biochemical Society Transactions
Volume	37
Issue number	Pt 1
Pages (from-to)	23-8
Number of pages	5
ISSN	0300-5127
DOIs	https://doi.org/10.1042/BST0370023
Publication status	Published - 2009

Access to Document

10.1042/BST0370023

Cite this

@article{220d09f0f86811ddb219000ea68e967b,

title = "Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism",

abstract = "Transcripts from spacer sequences within chromosomal repeat clusters [CRISPRs (clusters of regularly interspaced palindromic repeats)] from archaea have been implicated in inhibiting or regulating the propagation of archaeal viruses and plasmids. For the crenarchaeal thermoacidophiles, the chromosomal spacers show a high level of matches ( approximately 30%) with viral or plasmid genomes. Moreover, their distribution along the virus/plasmid genomes, as well as their DNA strand specificity, appear to be random. This is consistent with the hypothesis that chromosomal spacers are taken up directly and randomly from virus and plasmid DNA and that the spacer transcripts target the genomic DNA of the extrachromosomal elements and not their transcripts.",

author = "Shah, {Shiraz Ali} and Hansen, {Niels R} and Garrett, {Roger A}",

note = "Key words: acidothermophile, archaeal plasmid, archaeal virus, cluster of regularly interspaced palindromic repeats (CRISPR), crenarchaeon",

year = "2009",

doi = "10.1042/BST0370023",

language = "English",

volume = "37",

pages = "23--8",

journal = "Biochemical Society Transactions",

issn = "0300-5127",

publisher = "Portland Press Ltd.",

number = "Pt 1",

}

TY - JOUR

T1 - Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism

AU - Shah, Shiraz Ali

AU - Hansen, Niels R

AU - Garrett, Roger A

N1 - Key words: acidothermophile, archaeal plasmid, archaeal virus, cluster of regularly interspaced palindromic repeats (CRISPR), crenarchaeon

PY - 2009

Y1 - 2009

N2 - Transcripts from spacer sequences within chromosomal repeat clusters [CRISPRs (clusters of regularly interspaced palindromic repeats)] from archaea have been implicated in inhibiting or regulating the propagation of archaeal viruses and plasmids. For the crenarchaeal thermoacidophiles, the chromosomal spacers show a high level of matches ( approximately 30%) with viral or plasmid genomes. Moreover, their distribution along the virus/plasmid genomes, as well as their DNA strand specificity, appear to be random. This is consistent with the hypothesis that chromosomal spacers are taken up directly and randomly from virus and plasmid DNA and that the spacer transcripts target the genomic DNA of the extrachromosomal elements and not their transcripts.

AB - Transcripts from spacer sequences within chromosomal repeat clusters [CRISPRs (clusters of regularly interspaced palindromic repeats)] from archaea have been implicated in inhibiting or regulating the propagation of archaeal viruses and plasmids. For the crenarchaeal thermoacidophiles, the chromosomal spacers show a high level of matches ( approximately 30%) with viral or plasmid genomes. Moreover, their distribution along the virus/plasmid genomes, as well as their DNA strand specificity, appear to be random. This is consistent with the hypothesis that chromosomal spacers are taken up directly and randomly from virus and plasmid DNA and that the spacer transcripts target the genomic DNA of the extrachromosomal elements and not their transcripts.

U2 - 10.1042/BST0370023

DO - 10.1042/BST0370023

M3 - Journal article

C2 - 19143596

SN - 0300-5127

VL - 37

SP - 23

EP - 28

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - Pt 1

ER -

Distribution of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism

Abstract

Access to Document

Fingerprint

Cite this