Distinct gene expression profile of Xanthomonas retroflexus engaged in synergistic multispecies biofilm formation

TY - JOUR

T1 - Distinct gene expression profile of Xanthomonas retroflexus engaged in synergistic multispecies biofilm formation

AU - Hansen, Lea Benedicte Skov

AU - Ren, Dawei

AU - Burmølle, Mette

AU - Sørensen, Søren Johannes

PY - 2017/1/1

Y1 - 2017/1/1

N2 - It is well known that bacteria often exist in naturally formed multispecies biofilms. Within these biofilms, interspecies interactions seem to have an important role in ecological processes. Little is known about the effects of interspecies interactions on gene expression in these multispecies biofilms. This study presents a comparative gene expression analysis of the Xanthomonas retroflexus transcriptome when grown in a single-species biofilm and in dual-and four-species consortia with Stenotrophomonas rhizophila, Microbacterium oxydans and Paenibacillus amylolyticus. The results revealed complex interdependent interaction patterns in the multispecies biofilms. Many of the regulated functions are related to interactions with the external environment and suggest a high phenotypic plasticity in response to coexistence with other species. Furthermore, the changed expression of genes involved in aromatic and branched-chain amino acid biosynthesis suggests nutrient cross feeding as a contributing factor for the observed synergistic biofilm production when these four species coexists in a biofilm.

AB - It is well known that bacteria often exist in naturally formed multispecies biofilms. Within these biofilms, interspecies interactions seem to have an important role in ecological processes. Little is known about the effects of interspecies interactions on gene expression in these multispecies biofilms. This study presents a comparative gene expression analysis of the Xanthomonas retroflexus transcriptome when grown in a single-species biofilm and in dual-and four-species consortia with Stenotrophomonas rhizophila, Microbacterium oxydans and Paenibacillus amylolyticus. The results revealed complex interdependent interaction patterns in the multispecies biofilms. Many of the regulated functions are related to interactions with the external environment and suggest a high phenotypic plasticity in response to coexistence with other species. Furthermore, the changed expression of genes involved in aromatic and branched-chain amino acid biosynthesis suggests nutrient cross feeding as a contributing factor for the observed synergistic biofilm production when these four species coexists in a biofilm.

U2 - 10.1038/ismej.2016.107

DO - 10.1038/ismej.2016.107

M3 - Letter

C2 - 27505346

SN - 1751-7362

VL - 11

SP - 300

EP - 303

JO - I S M E Journal

JF - I S M E Journal

IS - 1

ER -