Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis

Adriana Jimena Bernal Giraldo; Jacob Krüger Jensen; Jesper Harholt; Susanne Oxenbøll Sørensen; Isabel Eva Moller; Claudia Blaukopf; Bo Buchholt Johansen; Robert Delotto; Markus Pauly; Henrik Vibe Scheller; William George Tycho Willats

doi:10.1111/j.1365-313X.2007.03281.x

Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis

Adriana Jimena Bernal Giraldo, Jacob Krüger Jensen, Jesper Harholt, Susanne Oxenbøll Sørensen, Isabel Eva Moller, Claudia Blaukopf, Bo Buchholt Johansen, Robert Delotto, Markus Pauly, Henrik Vibe Scheller, William George Tycho Willats

73 Citations (Scopus)

Abstract

Members of a large family of cellulose synthase-like genes (CSLs) are predicted to encode glycosyl transferases (GTs) involved in the biosynthesis of plant cell walls. The CSLA and CSLF families are known to contain mannan and glucan synthases, respectively, but the products of other CSLs are unknown. Here we report the effects of disrupting ATCSLD5 expression in Arabidopsis. Both stem and root growth were significantly reduced in ATCSLD5 knock-out plants, and these plants also had increased susceptibility to the cellulose synthase inhibitor isoxaben. Antibody and carbohydrate-binding module labelling indicated a reduction in the level of xylan in stems, and in vitro GT assays using microsomes from stems revealed that ATCSLD5 knock-out plants also had reduced xylan and homogalacturonan synthase activity. Expression in Nicotiana benthamiana of ATCSLD5 and ATCSLD3, fluorescently tagged at either the C- or the N-terminal, indicated that these GTs are likely to be localized in the Golgi apparatus. However, the position of the fluorescent tag affected the subcellular localization of both proteins. The work presented provides a comprehensive analysis of the effects of disrupting ATCSLD5 in planta, and the possible role(s) of this gene and other ATCSLDs in cell wall biosynthesis are discussed.

Original language	English
Journal	Plant Journal
Volume	52
Issue number	5
Pages (from-to)	791-802
Number of pages	12
ISSN	0960-7412
DOIs	https://doi.org/10.1111/j.1365-313X.2007.03281.x
Publication status	Published - 2007

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Bernal Giraldo, A. J., Jensen, J. K., Harholt, J., Sørensen, S. O., Moller, I. E., Blaukopf, C., Johansen, B. B., Delotto, R., Pauly, M., Scheller, H. V., & Willats, W. G. T. (2007). Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis. Plant Journal, 52(5), 791-802. https://doi.org/10.1111/j.1365-313X.2007.03281.x

Bernal Giraldo, AJ, Jensen, JK, Harholt, J, Sørensen, SO, Moller, IE, Blaukopf, C, Johansen, BB, Delotto, R, Pauly, M, Scheller, HV & Willats, WGT 2007, 'Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis', Plant Journal, vol. 52, no. 5, pp. 791-802. https://doi.org/10.1111/j.1365-313X.2007.03281.x

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title = "Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis",

abstract = "Members of a large family of cellulose synthase-like genes (CSLs) are predicted to encode glycosyl transferases (GTs) involved in the biosynthesis of plant cell walls. The CSLA and CSLF families are known to contain mannan and glucan synthases, respectively, but the products of other CSLs are unknown. Here we report the effects of disrupting ATCSLD5 expression in Arabidopsis. Both stem and root growth were significantly reduced in ATCSLD5 knock-out plants, and these plants also had increased susceptibility to the cellulose synthase inhibitor isoxaben. Antibody and carbohydrate-binding module labelling indicated a reduction in the level of xylan in stems, and in vitro GT assays using microsomes from stems revealed that ATCSLD5 knock-out plants also had reduced xylan and homogalacturonan synthase activity. Expression in Nicotiana benthamiana of ATCSLD5 and ATCSLD3, fluorescently tagged at either the C- or the N-terminal, indicated that these GTs are likely to be localized in the Golgi apparatus. However, the position of the fluorescent tag affected the subcellular localization of both proteins. The work presented provides a comprehensive analysis of the effects of disrupting ATCSLD5 in planta, and the possible role(s) of this gene and other ATCSLDs in cell wall biosynthesis are discussed.",

author = "{Bernal Giraldo}, {Adriana Jimena} and Jensen, {Jacob Kr{\"u}ger} and Jesper Harholt and S{\o}rensen, {Susanne Oxenb{\o}ll} and Moller, {Isabel Eva} and Claudia Blaukopf and Johansen, {Bo Buchholt} and Robert Delotto and Markus Pauly and Scheller, {Henrik Vibe} and Willats, {William George Tycho}",

year = "2007",

doi = "10.1111/j.1365-313X.2007.03281.x",

language = "English",

volume = "52",

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T1 - Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis

AU - Bernal Giraldo, Adriana Jimena

AU - Jensen, Jacob Krüger

AU - Harholt, Jesper

AU - Sørensen, Susanne Oxenbøll

AU - Moller, Isabel Eva

AU - Blaukopf, Claudia

AU - Johansen, Bo Buchholt

AU - Delotto, Robert

AU - Pauly, Markus

AU - Scheller, Henrik Vibe

AU - Willats, William George Tycho

PY - 2007

Y1 - 2007

N2 - Members of a large family of cellulose synthase-like genes (CSLs) are predicted to encode glycosyl transferases (GTs) involved in the biosynthesis of plant cell walls. The CSLA and CSLF families are known to contain mannan and glucan synthases, respectively, but the products of other CSLs are unknown. Here we report the effects of disrupting ATCSLD5 expression in Arabidopsis. Both stem and root growth were significantly reduced in ATCSLD5 knock-out plants, and these plants also had increased susceptibility to the cellulose synthase inhibitor isoxaben. Antibody and carbohydrate-binding module labelling indicated a reduction in the level of xylan in stems, and in vitro GT assays using microsomes from stems revealed that ATCSLD5 knock-out plants also had reduced xylan and homogalacturonan synthase activity. Expression in Nicotiana benthamiana of ATCSLD5 and ATCSLD3, fluorescently tagged at either the C- or the N-terminal, indicated that these GTs are likely to be localized in the Golgi apparatus. However, the position of the fluorescent tag affected the subcellular localization of both proteins. The work presented provides a comprehensive analysis of the effects of disrupting ATCSLD5 in planta, and the possible role(s) of this gene and other ATCSLDs in cell wall biosynthesis are discussed.

AB - Members of a large family of cellulose synthase-like genes (CSLs) are predicted to encode glycosyl transferases (GTs) involved in the biosynthesis of plant cell walls. The CSLA and CSLF families are known to contain mannan and glucan synthases, respectively, but the products of other CSLs are unknown. Here we report the effects of disrupting ATCSLD5 expression in Arabidopsis. Both stem and root growth were significantly reduced in ATCSLD5 knock-out plants, and these plants also had increased susceptibility to the cellulose synthase inhibitor isoxaben. Antibody and carbohydrate-binding module labelling indicated a reduction in the level of xylan in stems, and in vitro GT assays using microsomes from stems revealed that ATCSLD5 knock-out plants also had reduced xylan and homogalacturonan synthase activity. Expression in Nicotiana benthamiana of ATCSLD5 and ATCSLD3, fluorescently tagged at either the C- or the N-terminal, indicated that these GTs are likely to be localized in the Golgi apparatus. However, the position of the fluorescent tag affected the subcellular localization of both proteins. The work presented provides a comprehensive analysis of the effects of disrupting ATCSLD5 in planta, and the possible role(s) of this gene and other ATCSLDs in cell wall biosynthesis are discussed.

U2 - 10.1111/j.1365-313X.2007.03281.x

DO - 10.1111/j.1365-313X.2007.03281.x

M3 - Journal article

C2 - 17892446

SN - 0960-7412

VL - 52

SP - 791

EP - 802

JO - Plant Journal

JF - Plant Journal

IS - 5

ER -

Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis

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