Disentangling optically activated delayed fluorescence and upconversion fluorescence in DNA stabilized silver nanoclusters

Stefan Krause; Cecilia Cerretani; Tom Vosch

doi:10.1039/c9sc00865a

Disentangling optically activated delayed fluorescence and upconversion fluorescence in DNA stabilized silver nanoclusters

Stefan Krause, Cecilia Cerretani, Tom Vosch

Department of Chemistry

6 Citations (Scopus)

2 Downloads (Pure)

Abstract

Optically activated delayed fluorescence (OADF) is a powerful tool for generating background-free, anti-Stokes fluorescence microscopy modalities. Recent findings, using DNA-stabilized silver nanoclusters (DNA-AgNCs), indicate that OADF is usually accompanied by a dark state-mediated consecutive photon absorption process leading to upconversion fluorescence (UCF). In this study, we disentangle the OADF and UCF process by means of wavelength-dependent NIR excitation spectroscopy. We demonstrate that, by appropriate choice of secondary NIR excitation wavelength, the dark state population can be preferentially depleted through OADF, minimizing the UCF contribution. These findings show that dark state depletion by OADF might enable background-free STED-like nanoscopy.

Original language	English
Journal	Chemical Science
Volume	10
Issue number	20
Pages (from-to)	5326-5331
ISSN	2041-6520
DOIs	https://doi.org/10.1039/c9sc00865a
Publication status	Published - 2019

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Disentangling optically activated delayed fluorescence and upconversion fluorescence in DNA stabilized silver nanoclustersFinal published version, 1.29 MBLicence: CC BY

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title = "Disentangling optically activated delayed fluorescence and upconversion fluorescence in DNA stabilized silver nanoclusters",

abstract = "Optically activated delayed fluorescence (OADF) is a powerful tool for generating background-free, anti-Stokes fluorescence microscopy modalities. Recent findings, using DNA-stabilized silver nanoclusters (DNA-AgNCs), indicate that OADF is usually accompanied by a dark state-mediated consecutive photon absorption process leading to upconversion fluorescence (UCF). In this study, we disentangle the OADF and UCF process by means of wavelength-dependent NIR excitation spectroscopy. We demonstrate that, by appropriate choice of secondary NIR excitation wavelength, the dark state population can be preferentially depleted through OADF, minimizing the UCF contribution. These findings show that dark state depletion by OADF might enable background-free STED-like nanoscopy.",

author = "Stefan Krause and Cecilia Cerretani and Tom Vosch",

year = "2019",

doi = "10.1039/c9sc00865a",

language = "English",

volume = "10",

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journal = "Chemical Science",

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publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Disentangling optically activated delayed fluorescence and upconversion fluorescence in DNA stabilized silver nanoclusters

AU - Krause, Stefan

AU - Cerretani, Cecilia

AU - Vosch, Tom

PY - 2019

Y1 - 2019

N2 - Optically activated delayed fluorescence (OADF) is a powerful tool for generating background-free, anti-Stokes fluorescence microscopy modalities. Recent findings, using DNA-stabilized silver nanoclusters (DNA-AgNCs), indicate that OADF is usually accompanied by a dark state-mediated consecutive photon absorption process leading to upconversion fluorescence (UCF). In this study, we disentangle the OADF and UCF process by means of wavelength-dependent NIR excitation spectroscopy. We demonstrate that, by appropriate choice of secondary NIR excitation wavelength, the dark state population can be preferentially depleted through OADF, minimizing the UCF contribution. These findings show that dark state depletion by OADF might enable background-free STED-like nanoscopy.

AB - Optically activated delayed fluorescence (OADF) is a powerful tool for generating background-free, anti-Stokes fluorescence microscopy modalities. Recent findings, using DNA-stabilized silver nanoclusters (DNA-AgNCs), indicate that OADF is usually accompanied by a dark state-mediated consecutive photon absorption process leading to upconversion fluorescence (UCF). In this study, we disentangle the OADF and UCF process by means of wavelength-dependent NIR excitation spectroscopy. We demonstrate that, by appropriate choice of secondary NIR excitation wavelength, the dark state population can be preferentially depleted through OADF, minimizing the UCF contribution. These findings show that dark state depletion by OADF might enable background-free STED-like nanoscopy.

U2 - 10.1039/c9sc00865a

DO - 10.1039/c9sc00865a

M3 - Journal article

C2 - 31191889

SN - 2041-6520

VL - 10

SP - 5326

EP - 5331

JO - Chemical Science

JF - Chemical Science

IS - 20

ER -

Disentangling optically activated delayed fluorescence and upconversion fluorescence in DNA stabilized silver nanoclusters

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