Discovery of O-glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor

Lasse H. Hansen; Thomas Daugbjerg Madsen; Christoffer K. Goth; Henrik Clausen; Yang Chen; Nina Dzhoyashvili; Seethalakshmi R. Iyer; S. Jeson Sangaralingham; John C. Burnett; Jens F. Rehfeld; Sergey Y. Vakhrushev; Katrine T. Schjoldager; Jens P. Goetze

doi:10.1074/jbc.RA119.008102

Discovery of O-glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor

Lasse H. Hansen, Thomas Daugbjerg Madsen, Christoffer K. Goth, Henrik Clausen, Yang Chen, Nina Dzhoyashvili, Seethalakshmi R. Iyer, S. Jeson Sangaralingham, John C. Burnett, Jens F. Rehfeld, Sergey Y. Vakhrushev, Katrine T. Schjoldager^*, Jens P. Goetze

^*Corresponding author for this work

17 Citations (Scopus)

Abstract

Atrial natriuretic peptide (ANP) is a peptide hormone that in response to atrial stretch is secreted from atrial myocytes into the circulation, where it stimulates vasodilatation and natriuresis. ANP is an important biomarker of heart failure where low plasma concentrations exclude cardiac dysfunction. ANP is a member of the natriuretic peptide (NP) family, which also includes the B-type natriuretic peptide (BNP) and the C-type natriuretic peptide. The proforms of these hormones undergo processing to mature peptides, and for proBNP, this process has previously been demonstrated to be regulated by O-glycosylation. It has been suggested that proANP also may undergo posttranslational modifications. Here, we conducted a targeted O-glycoproteomics approach to characterize O-glycans on NPs and demonstrate that all NP members can carry O-glycans. We identified four O-glycosites in proANP in the porcine heart, and surprisingly, two of these were located on the mature bioactive ANP itself. We found that one of these glycans is located within a conserved sequence motif of the receptor-binding region, suggesting thatO-glycans may serve a function beyond intracellular processing and maturation. We also identified an O-glycoform of proANP naturally occurring in human circulation. We demonstrated that site-specific O-glycosylation shields bioactive ANP from proteolytic degradation and modifies potency at its cognate receptor in vitro. Furthermore, we showed that ANP O-glycosylation attenuates acute renal and cardiovascular ANP actions in vivo. The discovery of novel glycosylatedANPproteoforms reported here significantly improves our understanding of cardiac endocrinology and provides important insight into the etiology of heart failure.

Original language	English
Journal	Journal of Biological Chemistry
Volume	294
Issue number	34
Pages (from-to)	12567-12578
Number of pages	12
ISSN	0021-9258
DOIs	https://doi.org/10.1074/jbc.RA119.008102
Publication status	Published - 23 Aug 2019

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10.1074/jbc.RA119.008102

Discovery of O-glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor

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Hansen, L. H., Madsen, T. D., Goth, C. K., Clausen, H., Chen, Y., Dzhoyashvili, N., Iyer, S. R., Sangaralingham, S. J., Burnett, J. C., Rehfeld, J. F., Vakhrushev, S. Y., Schjoldager, K. T., & Goetze, J. P. (2019). Discovery of O-glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor. Journal of Biological Chemistry, 294(34), 12567-12578. https://doi.org/10.1074/jbc.RA119.008102

Hansen, LH, Madsen, TD , Goth, CK , Clausen, H, Chen, Y, Dzhoyashvili, N, Iyer, SR, Sangaralingham, SJ, Burnett, JC, Rehfeld, JF , Vakhrushev, SY , Schjoldager, KT & Goetze, JP 2019, 'Discovery of O-glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor', Journal of Biological Chemistry, vol. 294, no. 34, pp. 12567-12578. https://doi.org/10.1074/jbc.RA119.008102

@article{53b0fd78dc8d443d9fd0e1c5d1a5b41b,

title = "Discovery of O-glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor",

abstract = "Atrial natriuretic peptide (ANP) is a peptide hormone that in response to atrial stretch is secreted from atrial myocytes into the circulation, where it stimulates vasodilatation and natriuresis. ANP is an important biomarker of heart failure where low plasma concentrations exclude cardiac dysfunction. ANP is a member of the natriuretic peptide (NP) family, which also includes the B-type natriuretic peptide (BNP) and the C-type natriuretic peptide. The proforms of these hormones undergo processing to mature peptides, and for proBNP, this process has previously been demonstrated to be regulated by O-glycosylation. It has been suggested that proANP also may undergo posttranslational modifications. Here, we conducted a targeted O-glycoproteomics approach to characterize O-glycans on NPs and demonstrate that all NP members can carry O-glycans. We identified four O-glycosites in proANP in the porcine heart, and surprisingly, two of these were located on the mature bioactive ANP itself. We found that one of these glycans is located within a conserved sequence motif of the receptor-binding region, suggesting thatO-glycans may serve a function beyond intracellular processing and maturation. We also identified an O-glycoform of proANP naturally occurring in human circulation. We demonstrated that site-specific O-glycosylation shields bioactive ANP from proteolytic degradation and modifies potency at its cognate receptor in vitro. Furthermore, we showed that ANP O-glycosylation attenuates acute renal and cardiovascular ANP actions in vivo. The discovery of novel glycosylatedANPproteoforms reported here significantly improves our understanding of cardiac endocrinology and provides important insight into the etiology of heart failure.",

author = "Hansen, {Lasse H.} and Madsen, {Thomas Daugbjerg} and Goth, {Christoffer K.} and Henrik Clausen and Yang Chen and Nina Dzhoyashvili and Iyer, {Seethalakshmi R.} and Sangaralingham, {S. Jeson} and Burnett, {John C.} and Rehfeld, {Jens F.} and Vakhrushev, {Sergey Y.} and Schjoldager, {Katrine T.} and Goetze, {Jens P.}",

year = "2019",

month = aug,

day = "23",

doi = "10.1074/jbc.RA119.008102",

language = "English",

volume = "294",

pages = "12567--12578",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology, Inc.",

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T1 - Discovery of O-glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor

AU - Hansen, Lasse H.

AU - Madsen, Thomas Daugbjerg

AU - Goth, Christoffer K.

AU - Clausen, Henrik

AU - Chen, Yang

AU - Dzhoyashvili, Nina

AU - Iyer, Seethalakshmi R.

AU - Sangaralingham, S. Jeson

AU - Burnett, John C.

AU - Rehfeld, Jens F.

AU - Vakhrushev, Sergey Y.

AU - Schjoldager, Katrine T.

AU - Goetze, Jens P.

PY - 2019/8/23

Y1 - 2019/8/23

N2 - Atrial natriuretic peptide (ANP) is a peptide hormone that in response to atrial stretch is secreted from atrial myocytes into the circulation, where it stimulates vasodilatation and natriuresis. ANP is an important biomarker of heart failure where low plasma concentrations exclude cardiac dysfunction. ANP is a member of the natriuretic peptide (NP) family, which also includes the B-type natriuretic peptide (BNP) and the C-type natriuretic peptide. The proforms of these hormones undergo processing to mature peptides, and for proBNP, this process has previously been demonstrated to be regulated by O-glycosylation. It has been suggested that proANP also may undergo posttranslational modifications. Here, we conducted a targeted O-glycoproteomics approach to characterize O-glycans on NPs and demonstrate that all NP members can carry O-glycans. We identified four O-glycosites in proANP in the porcine heart, and surprisingly, two of these were located on the mature bioactive ANP itself. We found that one of these glycans is located within a conserved sequence motif of the receptor-binding region, suggesting thatO-glycans may serve a function beyond intracellular processing and maturation. We also identified an O-glycoform of proANP naturally occurring in human circulation. We demonstrated that site-specific O-glycosylation shields bioactive ANP from proteolytic degradation and modifies potency at its cognate receptor in vitro. Furthermore, we showed that ANP O-glycosylation attenuates acute renal and cardiovascular ANP actions in vivo. The discovery of novel glycosylatedANPproteoforms reported here significantly improves our understanding of cardiac endocrinology and provides important insight into the etiology of heart failure.

AB - Atrial natriuretic peptide (ANP) is a peptide hormone that in response to atrial stretch is secreted from atrial myocytes into the circulation, where it stimulates vasodilatation and natriuresis. ANP is an important biomarker of heart failure where low plasma concentrations exclude cardiac dysfunction. ANP is a member of the natriuretic peptide (NP) family, which also includes the B-type natriuretic peptide (BNP) and the C-type natriuretic peptide. The proforms of these hormones undergo processing to mature peptides, and for proBNP, this process has previously been demonstrated to be regulated by O-glycosylation. It has been suggested that proANP also may undergo posttranslational modifications. Here, we conducted a targeted O-glycoproteomics approach to characterize O-glycans on NPs and demonstrate that all NP members can carry O-glycans. We identified four O-glycosites in proANP in the porcine heart, and surprisingly, two of these were located on the mature bioactive ANP itself. We found that one of these glycans is located within a conserved sequence motif of the receptor-binding region, suggesting thatO-glycans may serve a function beyond intracellular processing and maturation. We also identified an O-glycoform of proANP naturally occurring in human circulation. We demonstrated that site-specific O-glycosylation shields bioactive ANP from proteolytic degradation and modifies potency at its cognate receptor in vitro. Furthermore, we showed that ANP O-glycosylation attenuates acute renal and cardiovascular ANP actions in vivo. The discovery of novel glycosylatedANPproteoforms reported here significantly improves our understanding of cardiac endocrinology and provides important insight into the etiology of heart failure.

U2 - 10.1074/jbc.RA119.008102

DO - 10.1074/jbc.RA119.008102

M3 - Review

C2 - 31186350

AN - SCOPUS:85071484729

SN - 0021-9258

VL - 294

SP - 12567

EP - 12578

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 34

ER -

Discovery of O-glycans on atrial natriuretic peptide (ANP) that affect both its proteolytic degradation and potency at its cognate receptor

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