Differential effects of low-temperature inhibition on the propylene induced autocatalysis of ethylene production, respiration and ripening of 'Hayward' kiwifruit

TY - JOUR

T1 - Differential effects of low-temperature inhibition on the propylene induced autocatalysis of ethylene production, respiration and ripening of 'Hayward' kiwifruit

AU - Antunes, M. D C

AU - Pateraki, I.

AU - Kanellis, A. K.

AU - Sfakiotakis, E. M.

AU - Pateraki, Eirini

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Previous studies (Stavroulakis and Sfakiotakis, 1993) have shown an inhibition of propylene-induced ethylene production in kiwifruit below a critical temperature range of 11-14.8°C. The aim of this research was to identify the biochemical basis of this inhibition in kiwifruit below 11-14.8°C. 'Hayward' kiwifruit were treated with increasing propylene concentrations at 10 and 20°C. Ethylene biosynthesis pathways and fruit ripening were investigated. Kiwifruit at 20°C in air started autocatalysis of ethylene production and ripened after 19 d with a concomitant increase in respiration. Ethylene production and the respiration rise appeared earlier with increased propylene concentrations. Ripening proceeded immediately after propylene treatment, while ethylene autocatalysis needed a lag period of 24-72 h. The latter event was attributed to the delay found in the induction of 1-aminocyclopropane-1-carboxylate synthase (ACC synthase) activity and consequently to the delayed increase of 1-aminocyclopropane-1-carboxylic acid (ACC) content. In contrast propylene treatment induced 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase) activity with no lag period. Moreover, transcription of ACC synthase and ACC oxidase genes was active only in ethylene-producing kiwifruit at 20°C. In contrast, treatment at 10°C with propylene strongly inhibited ethylene production, which was attributed to the low activities of both ACC synthase and ACC oxidase as well as the low initial ACC level. Interestingly, fruit treated with propylene at 10°C appeared to be able to transcribe the ACC oxidase but not the ACC synthase gene. However, propylene induced ripening of that fruit almost as rapidly as in the propylene-treated fruit at 20°C. Respiration rate was increased together with propylene concentration. It is concluded that kiwifruit stored at 20°C behaves as a typical climacteric fruit, while at 10°C behaves like a non-climacteric fruit. We propose that the main reasons for the inhibition of the propylene induced (autocatalytic) ethylene production in kiwifruit at low temperature (≤ IO°C), are primarily the suppression of the propylene-induced ACC synthase gene expression and the possible post-transcriptional modification of ACC oxidase.

AB - Previous studies (Stavroulakis and Sfakiotakis, 1993) have shown an inhibition of propylene-induced ethylene production in kiwifruit below a critical temperature range of 11-14.8°C. The aim of this research was to identify the biochemical basis of this inhibition in kiwifruit below 11-14.8°C. 'Hayward' kiwifruit were treated with increasing propylene concentrations at 10 and 20°C. Ethylene biosynthesis pathways and fruit ripening were investigated. Kiwifruit at 20°C in air started autocatalysis of ethylene production and ripened after 19 d with a concomitant increase in respiration. Ethylene production and the respiration rise appeared earlier with increased propylene concentrations. Ripening proceeded immediately after propylene treatment, while ethylene autocatalysis needed a lag period of 24-72 h. The latter event was attributed to the delay found in the induction of 1-aminocyclopropane-1-carboxylate synthase (ACC synthase) activity and consequently to the delayed increase of 1-aminocyclopropane-1-carboxylic acid (ACC) content. In contrast propylene treatment induced 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase) activity with no lag period. Moreover, transcription of ACC synthase and ACC oxidase genes was active only in ethylene-producing kiwifruit at 20°C. In contrast, treatment at 10°C with propylene strongly inhibited ethylene production, which was attributed to the low activities of both ACC synthase and ACC oxidase as well as the low initial ACC level. Interestingly, fruit treated with propylene at 10°C appeared to be able to transcribe the ACC oxidase but not the ACC synthase gene. However, propylene induced ripening of that fruit almost as rapidly as in the propylene-treated fruit at 20°C. Respiration rate was increased together with propylene concentration. It is concluded that kiwifruit stored at 20°C behaves as a typical climacteric fruit, while at 10°C behaves like a non-climacteric fruit. We propose that the main reasons for the inhibition of the propylene induced (autocatalytic) ethylene production in kiwifruit at low temperature (≤ IO°C), are primarily the suppression of the propylene-induced ACC synthase gene expression and the possible post-transcriptional modification of ACC oxidase.

M3 - Journal article

AN - SCOPUS:0033832521

SN - 1462-0316

VL - 75

SP - 575

EP - 580

JO - Journal of Horticultural Science and Biotechnology

JF - Journal of Horticultural Science and Biotechnology

IS - 5

ER -