Abstract
The effect of extracellular calcium on the Na(+)-K+ pump activity in human polymorphonuclear leucocytes and erythrocytes was studied and compared with the activity in mixed peritoneal leucocytes from rats. While there was maximal decrease in the pump activity (25-30%) of leucocytes from both rat and human by calcium 0.6 mM, a concentration of 0.1 mM caused a substantial decrease indicating a high sensitivity for extracellular calcium. In contrast, calcium had no effect on the pump activity in erythrocytes. The effect of calcium on the pump activity in leucocytes may be due to regulation of the influx of sodium across the plasma membrane, since in human leucocytes calcium had no effect on the pump activity if the cells were loaded with sodium.
| Original language | English |
|---|---|
| Journal | FEBS Letters |
| Volume | 279 |
| Issue number | 1 |
| Pages (from-to) | 153-6 |
| Number of pages | 4 |
| ISSN | 0014-5793 |
| DOIs | |
| Publication status | Published - 11 Feb 1991 |
Keywords
- Animals
- Calcium/pharmacology
- Carrier Proteins/drug effects
- Erythrocytes/metabolism
- Humans
- Male
- Membrane Proteins/metabolism
- Neutrophils/metabolism
- Potassium/metabolism
- Rats
- Rats, Inbred Strains
- Sodium/metabolism
- Sodium-Potassium-Chloride Symporters