TY - JOUR
T1 - Differences in transcriptional activity of cutaneous human papillomaviruses
AU - Vasiljevic, Natasa
AU - Nielsen, Lone
AU - Doherty, Geoff
AU - Dillner, Joakim
AU - Forslund, Ola
AU - Norrild, Bodil
N1 - Keywords: Alphapapillomavirus; Base Sequence; Cell Line, Tumor; Gene Expression Regulation, Viral; Humans; Molecular Sequence Data; Papillomavirus Infections; Promoter Regions, Genetic; Skin; Transcription Initiation Site; Transcription, Genetic; Ultraviolet Rays
PY - 2008
Y1 - 2008
N2 - The interaction between UV-B irradiation and cutaneous human papillomaviruses (HPV) has been suggested to be of relevance for the development of non-melanoma skin cancers. We investigated the activity within the upstream regulatory region (URR) of the HPV types 8, 38, 92, 93 and 96, as well as their responsiveness to UV-B irradiation and cellular differentiation. Promoter activities were higher in HaCaT than in SiHa cells, corresponding to the HPV tissue tropism. Transcriptional start sites were mapped at P(92) (HPV-38), P(45) (HPV-92), P(7439) (HPV-93) and P(256) (HPV-96). Transcription from HPV-8, 93 and 96 URR was up-regulated by cellular differentiation, linking the activity of these HPVs to the cellular state. UV-B irradiation activated HPV-8 but inhibited HPV-38 and HPV-93 whereas HPV-92 and 96 were not affected. As there are variable UV-B responses among the HPV types, further studies of interactions between UV-B and HPV need to consider the HPV type.
AB - The interaction between UV-B irradiation and cutaneous human papillomaviruses (HPV) has been suggested to be of relevance for the development of non-melanoma skin cancers. We investigated the activity within the upstream regulatory region (URR) of the HPV types 8, 38, 92, 93 and 96, as well as their responsiveness to UV-B irradiation and cellular differentiation. Promoter activities were higher in HaCaT than in SiHa cells, corresponding to the HPV tissue tropism. Transcriptional start sites were mapped at P(92) (HPV-38), P(45) (HPV-92), P(7439) (HPV-93) and P(256) (HPV-96). Transcription from HPV-8, 93 and 96 URR was up-regulated by cellular differentiation, linking the activity of these HPVs to the cellular state. UV-B irradiation activated HPV-8 but inhibited HPV-38 and HPV-93 whereas HPV-92 and 96 were not affected. As there are variable UV-B responses among the HPV types, further studies of interactions between UV-B and HPV need to consider the HPV type.
U2 - 10.1016/j.virusres.2008.07.013
DO - 10.1016/j.virusres.2008.07.013
M3 - Letter
C2 - 18692100
SN - 0168-1702
VL - 137
SP - 213
EP - 219
JO - Virus Research
JF - Virus Research
IS - 2
ER -
