Differences in motility pattern between human buccal fibroblasts and periodontal and skin fibroblasts

TY - JOUR

T1 - Differences in motility pattern between human buccal fibroblasts and periodontal and skin fibroblasts

AU - Lepekhin, Eugene

AU - Grøn, Birgitte

AU - Berezin, Vladimir

AU - Bock, Elisabeth

AU - Dabelsteen, Erik

PY - 2002/2

Y1 - 2002/2

N2 - Migration of fibroblasts from surrounding normal tissue into the wound bed is an important requirement for successful wound healing. This study investigated the motility pattern of buccal, periodontal and skin fibroblasts to determine whether differences in the wound healing efficiency at these sites can be explained by differences in the motile behavior of their respective fibroblast populations. The migratory characteristics were studied in a two-dimensional culture system. The migration of single cells was time-lapse video recorded at intervals of 15 min for a period of 6 h using a computer-assisted microscope work-station. For evaluation of cell morphology, cell contours were recognized semiautomatically and used for determination of cell area, cell spreading and number and length of processes. We found that the cellular displacement of the buccal fibroblasts was only approximately 50% of the cellular displacement of periodontal and skin fibroblasts. The decreased cellular displacement of the buccal fibroblasts was found to be due to both lower cellular speed and less persistence in direction. The buccal fibroblasts also displayed smaller areas and longer processes. The differences in cellular morphology and motility pattern amongst the three fibroblast types could not be explained by differences in secretion of extracellular matrix components and are therefore believed to reflect phenotypic differences amongst fibroblast subpopulations.

AB - Migration of fibroblasts from surrounding normal tissue into the wound bed is an important requirement for successful wound healing. This study investigated the motility pattern of buccal, periodontal and skin fibroblasts to determine whether differences in the wound healing efficiency at these sites can be explained by differences in the motile behavior of their respective fibroblast populations. The migratory characteristics were studied in a two-dimensional culture system. The migration of single cells was time-lapse video recorded at intervals of 15 min for a period of 6 h using a computer-assisted microscope work-station. For evaluation of cell morphology, cell contours were recognized semiautomatically and used for determination of cell area, cell spreading and number and length of processes. We found that the cellular displacement of the buccal fibroblasts was only approximately 50% of the cellular displacement of periodontal and skin fibroblasts. The decreased cellular displacement of the buccal fibroblasts was found to be due to both lower cellular speed and less persistence in direction. The buccal fibroblasts also displayed smaller areas and longer processes. The differences in cellular morphology and motility pattern amongst the three fibroblast types could not be explained by differences in secretion of extracellular matrix components and are therefore believed to reflect phenotypic differences amongst fibroblast subpopulations.

KW - Actins

KW - Adult

KW - Algorithms

KW - Cell Count

KW - Cell Culture Techniques

KW - Cell Movement

KW - Cell Size

KW - Collagen Type I

KW - Coloring Agents

KW - Extracellular Matrix

KW - Fibroblasts

KW - Humans

KW - Image Processing, Computer-Assisted

KW - Immunohistochemistry

KW - Microscopy, Video

KW - Mouth Mucosa

KW - Periodontal Ligament

KW - Skin

KW - Statistics as Topic

KW - Time Factors

KW - Video Recording

KW - Wound Healing

M3 - Journal article

C2 - 11878755

SN - 0909-8836

VL - 110

SP - 13

EP - 20

JO - European Journal of Oral Sciences

JF - European Journal of Oral Sciences

IS - 1

ER -